Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P3F89_RS07355 Genome accession   NZ_CP119875
Coordinates   1392141..1392575 (+) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus tropicus strain T36S-23     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1387141..1397575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3F89_RS07335 (P3F89_07330) - 1388036..1389193 (-) 1158 WP_000434566.1 aminotransferase A -
  P3F89_RS07340 (P3F89_07335) - 1389317..1389517 (-) 201 WP_000929285.1 hypothetical protein -
  P3F89_RS07345 (P3F89_07340) kinB 1389827..1391101 (+) 1275 WP_000420393.1 sporulation sensor histidine kinase KinB -
  P3F89_RS07350 (P3F89_07345) - 1391155..1391904 (-) 750 WP_309573843.1 DUF3967 domain-containing protein -
  P3F89_RS07355 (P3F89_07350) nucA/comI 1392141..1392575 (+) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  P3F89_RS07360 (P3F89_07355) comJ 1392611..1393000 (+) 390 WP_002194051.1 competence protein ComJ -
  P3F89_RS07365 (P3F89_07360) metE 1393038..1395326 (-) 2289 WP_002194050.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  P3F89_RS07370 (P3F89_07365) - 1395973..1396404 (+) 432 WP_002194049.1 NUDIX hydrolase -
  P3F89_RS07375 (P3F89_07370) - 1396586..1397143 (-) 558 WP_033691464.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=801208 P3F89_RS07355 WP_000811492.1 1392141..1392575(+) (nucA/comI) [Bacillus tropicus strain T36S-23]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=801208 P3F89_RS07355 WP_000811492.1 1392141..1392575(+) (nucA/comI) [Bacillus tropicus strain T36S-23]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGCGATGAATGGCCAATGGCGATGTGTAAAGAAGGTGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCCGATAATCGCGGAGCAGGTTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556