Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   P3F88_RS16455 Genome accession   NZ_CP119873
Coordinates   3685815..3687332 (+) Length   505 a.a.
NCBI ID   WP_309583795.1    Uniprot ID   -
Organism   Paraburkholderia phenoliruptrix strain T36S-14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3680815..3692332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3F88_RS16430 (P3F88_16435) kdsD 3681940..3682923 (+) 984 WP_013587053.1 arabinose 5-phosphate isomerase KdsD -
  P3F88_RS16435 (P3F88_16440) - 3682923..3683483 (+) 561 WP_013587052.1 HAD family hydrolase -
  P3F88_RS16440 (P3F88_16445) lptC 3683480..3684091 (+) 612 WP_013587051.1 LPS export ABC transporter periplasmic protein LptC -
  P3F88_RS16445 (P3F88_16450) lptA 3684112..3684783 (+) 672 WP_028361629.1 lipopolysaccharide transport periplasmic protein LptA -
  P3F88_RS16450 (P3F88_16455) lptB 3684780..3685565 (+) 786 WP_013587049.1 LPS export ABC transporter ATP-binding protein -
  P3F88_RS16455 (P3F88_16460) rpoN/rpoN1 3685815..3687332 (+) 1518 WP_309583795.1 RNA polymerase factor sigma-54 Machinery gene
  P3F88_RS16460 (P3F88_16465) hpf 3687504..3687860 (+) 357 WP_006048027.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  P3F88_RS16465 (P3F88_16470) ptsN 3688122..3688637 (+) 516 WP_013587047.1 PTS IIA-like nitrogen regulatory protein PtsN -
  P3F88_RS16470 (P3F88_16475) hprK 3688777..3689745 (+) 969 WP_006048029.1 HPr(Ser) kinase/phosphatase -
  P3F88_RS16475 (P3F88_16480) rapZ 3689849..3690742 (+) 894 WP_013337957.1 RNase adapter RapZ -
  P3F88_RS16480 (P3F88_16485) - 3690782..3691417 (+) 636 WP_013587045.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55274.30 Da        Isoelectric Point: 5.0000

>NTDB_id=801186 P3F88_RS16455 WP_309583795.1 3685815..3687332(+) (rpoN/rpoN1) [Paraburkholderia phenoliruptrix strain T36S-14]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAISQNPLLENEDDWIASPLRVAADGSLIAQAPGSATPPDQM
GGNGSSSSTSSSERSENGEPQGVDEYNGLSGDGNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVT
QAGQRDRALVTFLIESLDDDGYLAATLEEVLADLPEELEVDLDELNAALALLHSFDPAGVGARSASECLKLQLLRLEPSP
TRALALDIVAHYLELLAARDFTRLRKHLKASDDDLRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENAKTPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=801186 P3F88_RS16455 WP_309583795.1 3685815..3687332(+) (rpoN/rpoN1) [Paraburkholderia phenoliruptrix strain T36S-14]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGTTGACCCCGCAACTGCAGCAGTCCATCCGGCTGCTTCA
GCTGTCTACGCTCGAACTGCAGCAGGAAGTGGCCACCGCGATCTCGCAAAACCCGCTCCTCGAGAACGAAGACGACTGGA
TCGCGAGCCCGCTTCGCGTGGCGGCCGACGGCTCGCTGATCGCGCAGGCGCCCGGCTCCGCCACCCCGCCCGACCAGATG
GGCGGCAACGGCTCGTCGTCGTCCACGAGCAGCAGCGAGCGCTCTGAGAACGGCGAGCCGCAAGGCGTGGACGAGTACAA
CGGCCTCTCTGGCGACGGCAACGGCGACGCGTCGCAATGGAATCTCGACGACTACGGCCGCTCCGGCAACGCCTCGGACG
ACGACGACCTGCCGCCGCTGCAAATTCACGAATCGAGTACTTCACTGCGCGATCACCTGATGGCGCAATTGCGCGTCACG
CAGGCCGGCCAGCGCGACCGGGCGCTCGTCACTTTTCTGATCGAATCGCTCGACGACGACGGCTATCTCGCCGCCACCCT
CGAAGAAGTGCTCGCCGACCTGCCCGAAGAGCTGGAAGTCGACCTCGACGAACTGAATGCGGCGCTAGCACTGCTGCACA
GCTTCGACCCAGCGGGCGTCGGGGCGCGCTCCGCGTCCGAGTGCCTGAAGCTGCAATTATTGCGGCTCGAGCCGTCGCCT
ACGCGCGCGCTCGCGCTCGACATCGTTGCCCATTATCTGGAACTGCTCGCCGCGCGCGATTTCACCCGCCTGCGCAAGCA
TCTGAAAGCAAGCGACGACGACCTGCGCGACGCCCACGCGCTCATCCGCTCGCTGGAACCATTCCCGGGCGCGGCCTACG
GCAAGGCCGAGGCGGATTACGTCGTGCCGGACATCATGGTGCGTAAAACCGCCCAGGGCTGGCAGGCCGAACTCAATCCC
GAAGTGGTGCCGAAACTGCGCATCAACCACCTGTACGCCAATATTTTGCGCAACAACCGGGGCGATCCCGGCAGCGGTTC
GTTGCGCCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGTTTCGAAACAATCCTGCGTGTCGCGC
AGGCCATTGTCGAGCGTCAAAAGAGCTTTTTTGTGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGAGAAATTGCT
GATACGCTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGTAAGTACATGCTCACCCCGTTCGGGACGCTCGA
GTTCAAATACTTCTTCGGTTCGCATGTTTCGACGGACACGGGCGGCGCGGCGTCTTCAACGGCCATTCGCGCGCTCATCA
AGCAACTGATAGGAGCGGAAAACGCAAAAACGCCTCTCTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCACGGCGCACCGTGGCGAAGTATCGCGAAGCGCTCAAGATTCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-332)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.798

100

0.673