Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   L6486_RS25685 Genome accession   NZ_AP022946
Coordinates   5014734..5016083 (-) Length   449 a.a.
NCBI ID   WP_000225359.1    Uniprot ID   -
Organism   Bacillus cereus strain J10     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5009734..5021083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6486_RS25650 (BCJMU10_5081) - 5009904..5010598 (-) 695 Protein_5007 hypothetical protein -
  L6486_RS25655 - 5010585..5011372 (-) 788 Protein_5008 hypothetical protein -
  L6486_RS25660 (BCJMU10_5084) - 5011400..5011969 (-) 570 WP_001211060.1 hypothetical protein -
  L6486_RS25665 (BCJMU10_5085) - 5012087..5012569 (-) 483 WP_000023374.1 hypothetical protein -
  L6486_RS25670 (BCJMU10_5086) raiA 5012841..5013383 (-) 543 WP_000671185.1 ribosome-associated translation inhibitor RaiA -
  L6486_RS25675 (BCJMU10_5087) cspC 5013706..5013903 (-) 198 WP_001990088.1 cold shock protein CspC -
  L6486_RS25680 (BCJMU10_5088) - 5014030..5014734 (-) 705 WP_033693505.1 ComF family protein -
  L6486_RS25685 (BCJMU10_5089) comFA 5014734..5016083 (-) 1350 WP_000225359.1 ATP-dependent helicase ComFA Machinery gene
  L6486_RS25690 (BCJMU10_5091) - 5016210..5017655 (-) 1446 WP_000499476.1 C40 family peptidase -
  L6486_RS25695 (BCJMU10_5092) - 5017804..5018118 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  L6486_RS25700 (BCJMU10_5093) - 5018291..5019133 (-) 843 WP_000684725.1 DegV family protein -
  L6486_RS25705 (BCJMU10_5094) - 5019370..5020005 (+) 636 WP_000926669.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51115.96 Da        Isoelectric Point: 9.9910

>NTDB_id=80118 L6486_RS25685 WP_000225359.1 5014734..5016083(-) (comFA) [Bacillus cereus strain J10]
MVAGKQLLLEELSSDLQRKLNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKNSNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVIVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKFRKGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLNRERIPRVLLQWLKMYLNKKYPVFL
FVPHVRYIEEISSLLKSLHNKVEGVHAEDPMRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=80118 L6486_RS25685 WP_000225359.1 5014734..5016083(-) (comFA) [Bacillus cereus strain J10]
ATGGTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGAAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGTGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGAGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTCAAATTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCAAGAACGGATGTTGTTTTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTAGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATAGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAAAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCGTTGCCAGTTCCTCTATTTTGCTGGTGCGGAAATTGGAAGAAAA
GCCTCAACCGTGAAAGAATTCCTCGAGTTTTACTACAATGGTTAAAGATGTACTTAAACAAAAAGTATCCTGTTTTTTTA
TTCGTTCCCCATGTACGATATATAGAAGAAATAAGCTCGTTATTGAAATCATTGCATAATAAAGTTGAAGGGGTACATGC
AGAAGATCCGATGAGAAAAGAGAAAGTCGCAGCGTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCATATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

52.632

93.096

0.49

Multiple sequence alignment