Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   L6486_RS19515 Genome accession   NZ_AP022946
Coordinates   3850439..3850873 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain J10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3845439..3855873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6486_RS19500 (BCJMU10_3863) - 3846615..3847172 (+) 558 WP_000062067.1 PadR family transcriptional regulator -
  L6486_RS19505 (BCJMU10_3864) metE 3847686..3849974 (+) 2289 WP_236789750.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  L6486_RS19510 (BCJMU10_3865) comJ 3850013..3850402 (-) 390 WP_000424030.1 competence protein ComJ -
  L6486_RS19515 (BCJMU10_3866) nucA/comI 3850439..3850873 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  L6486_RS19520 (BCJMU10_3867) - 3851100..3851849 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  L6486_RS19525 (BCJMU10_3868) kinB 3851888..3853162 (-) 1275 WP_000420370.1 sporulation sensor histidine kinase KinB -
  L6486_RS19530 (BCJMU10_3869) - 3853473..3853673 (+) 201 WP_000929278.1 hypothetical protein -
  L6486_RS19535 (BCJMU10_3870) - 3853796..3854959 (+) 1164 WP_000434582.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=80105 L6486_RS19515 WP_000811494.1 3850439..3850873(-) (nucA/comI) [Bacillus cereus strain J10]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=80105 L6486_RS19515 WP_000811494.1 3850439..3850873(-) (nucA/comI) [Bacillus cereus strain J10]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGTTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment