Detailed information NTG0080068

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: L6482_RS25990
• Genome accession: NZ_AP022934
• Coordinates: 4998525..4999874 (-)
• Length: 449 a.a.
• NCBI ID: WP_061531556.1
• Uniprot ID: -
• Organism: Bacillus cereus strain J7
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4993525..5004874

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6482_RS25970 (BCJMU07_5170)	secA	4993847..4996354 (-)	2508	WP_000579383.1	preprotein translocase subunit SecA	-
L6482_RS25975 (BCJMU07_5171)	raiA	4996631..4997173 (-)	543	WP_000671189.1	ribosome-associated translation inhibitor RaiA	-
L6482_RS25980 (BCJMU07_5172)	cspC	4997497..4997694 (-)	198	WP_001990088.1	cold shock protein CspC	-
L6482_RS25985 (BCJMU07_5173)	comFC	4997821..4998525 (-)	705	WP_041184339.1	comF operon protein ComFC	-
L6482_RS25990 (BCJMU07_5174)	comFA	4998525..4999874 (-)	1350	WP_061531556.1	ATP-dependent helicase ComFA	Machinery gene
L6482_RS25995 (BCJMU07_5175)	-	5000002..5001312 (-)	1311	WP_000734330.1	C40 family peptidase	-
L6482_RS26000 (BCJMU07_5176)	-	5001566..5001880 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
L6482_RS26005 (BCJMU07_5177)	-	5002054..5002896 (-)	843	WP_000844766.1	DegV family protein	-
L6482_RS26010 (BCJMU07_5178)	-	5003136..5003771 (+)	636	WP_000926685.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51370.06 Da
• Isoelectric Point: 9.7210

>NTDB_id=80068 L6482_RS25990 WP_061531556.1 4998525..4999874(-) (comFA) [Bacillus cereus strain J7]
MVAGKQLLLEELSSDLRRELSDLKKKGEIICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGTLSTGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKEERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KERAARIYLTATPDEKWKRNFRRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEMSLLLKGLDHRIDGVHAEDPMRKEKVAAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=80068 L6482_RS25990 WP_061531556.1 4998525..4999874(-) (comFA) [Bacillus cereus strain J7]
ATGGTTGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAAGGG
AGAGATTATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGAATTCATGAAAGAAAAGGAGAAAGAGAATTACATTCGTTACAGTGGAAAGGAACTTT
GTCTACTGGTCAGGAGTTGGCAGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCGTTTTTTATTTGGGCGGTAT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGAAGAAAGAGTTTGTATTGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCATTCC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAACAAGCGATG
AAAGAGAGAGCGGCGCGTATTTATTTAACTGCAACCCCGGATGAAAAGTGGAAGCGCAATTTCAGAAGGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGTAATTGGAAGAAAA
GCCTTCATCATAAAAAAATTCCTCGAGTGTTACTACAATGGTTGAAAATGTACGTAAACAAAAAATATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATGAGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAGGAAAAAGTGGCAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCACTACGGCAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

• Type III restriction enzyme, res subunit (ResIII) (107-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.914	93.096	0.483
comFA	Latilactobacillus sakei subsp. sakei 23K	40.5	89.087	0.361