Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P3K36_RS06690 Genome accession   NZ_CP119688
Coordinates   1396685..1397659 (+) Length   324 a.a.
NCBI ID   WP_014613820.1    Uniprot ID   -
Organism   Staphylococcus pseudintermedius strain CUVET18-875     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1391685..1402659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3K36_RS06655 (P3K36_06600) rpmG 1392085..1392234 (+) 150 WP_014613813.1 50S ribosomal protein L33 -
  P3K36_RS06660 (P3K36_06605) - 1392386..1392925 (+) 540 WP_203160814.1 5-formyltetrahydrofolate cyclo-ligase -
  P3K36_RS06665 (P3K36_06610) - 1392944..1394383 (+) 1440 WP_203200851.1 rhomboid family intramembrane serine protease -
  P3K36_RS06670 (P3K36_06615) - 1394385..1394588 (+) 204 WP_014613816.1 YqgQ family protein -
  P3K36_RS06675 (P3K36_06620) - 1394585..1395571 (+) 987 WP_014613817.1 ROK family glucokinase -
  P3K36_RS06680 (P3K36_06625) - 1395572..1395883 (+) 312 WP_014613818.1 MTH1187 family thiamine-binding protein -
  P3K36_RS06685 (P3K36_06630) - 1395896..1396525 (+) 630 WP_096535719.1 MBL fold metallo-hydrolase -
  P3K36_RS06690 (P3K36_06635) comGA 1396685..1397659 (+) 975 WP_014613820.1 competence type IV pilus ATPase ComGA Machinery gene
  P3K36_RS06695 (P3K36_06640) comGB 1397628..1398695 (+) 1068 WP_015729166.1 competence type IV pilus assembly protein ComGB -
  P3K36_RS06700 (P3K36_06645) comGC 1398708..1399031 (+) 324 WP_015729165.1 competence type IV pilus major pilin ComGC Machinery gene
  P3K36_RS06705 (P3K36_06650) comGD 1399009..1399449 (+) 441 WP_015729164.1 competence type IV pilus minor pilin ComGD -
  P3K36_RS06710 (P3K36_06655) - 1399554..1399754 (+) 201 WP_238402310.1 hypothetical protein -
  P3K36_RS06715 (P3K36_06660) - 1399660..1400145 (+) 486 WP_080552896.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P3K36_RS06720 (P3K36_06665) - 1400309..1400836 (+) 528 WP_140241608.1 shikimate kinase -
  P3K36_RS06725 (P3K36_06670) gcvT 1400994..1402085 (+) 1092 WP_015729161.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36846.58 Da        Isoelectric Point: 7.5584

>NTDB_id=800393 P3K36_RS06690 WP_014613820.1 1396685..1397659(+) (comGA) [Staphylococcus pseudintermedius strain CUVET18-875]
MQLLLDHVIQFALQNEASDIHFIPSQSQVEVKLRVKDQLVLYDTLNLETYQKLLTLLKYQAGLDISTRHKAQSGRYIYEH
KALYYLRVSTLPLNLGTESCVIRITPQYFQNAKAHSVLDIKSNMEKKQGLILFSGPTGAGKSTLMYQMVVYAKQKLNLNI
ITIEDPVEQLVNGITQISVNEKADITYGSSLKAILRCDPDVILIGEIRDSHIARDVIQASLSGHLVLSTIHANDCQGVLL
RLIEMGLSHQDLSQSINLIINQRLITTKNDQRQLVYETMDQQEIHYFLNNNFQLPTAFTSLSHKLQLLNKEGVIDEKILR
KYTI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=800393 P3K36_RS06690 WP_014613820.1 1396685..1397659(+) (comGA) [Staphylococcus pseudintermedius strain CUVET18-875]
ATGCAACTACTATTAGATCATGTAATACAATTTGCACTTCAAAATGAGGCGTCAGACATTCATTTTATCCCATCACAATC
ACAAGTCGAAGTGAAACTCAGAGTGAAAGATCAACTTGTATTATATGACACATTAAATCTAGAAACGTATCAAAAATTGT
TAACTTTGTTGAAATACCAAGCTGGATTAGATATTTCGACACGCCACAAAGCGCAAAGTGGGCGCTATATTTATGAACAT
AAAGCGTTATATTATTTAAGAGTGTCCACGTTACCTTTAAACTTAGGTACTGAAAGTTGTGTTATTCGTATTACACCCCA
ATATTTTCAAAATGCGAAAGCTCATAGTGTATTAGACATCAAATCTAATATGGAGAAAAAGCAAGGTTTAATTCTGTTTA
GCGGGCCTACAGGTGCAGGAAAGAGCACGTTAATGTATCAAATGGTCGTCTATGCTAAACAAAAGTTAAACCTGAATATT
ATCACAATAGAAGACCCGGTCGAGCAGCTTGTGAATGGCATTACACAAATTTCCGTTAATGAAAAAGCTGATATTACGTA
TGGATCATCGTTAAAAGCAATTCTTCGTTGTGATCCAGATGTCATCTTAATTGGTGAGATTCGTGACAGTCATATTGCAA
GGGACGTTATACAAGCGAGTTTAAGCGGGCATTTAGTATTGTCGACGATACACGCCAATGATTGTCAAGGGGTGCTCCTT
AGACTGATTGAAATGGGACTCTCTCACCAAGATTTATCTCAATCTATCAATCTCATCATCAATCAAAGACTCATTACAAC
AAAAAACGACCAGCGACAACTCGTCTATGAAACGATGGATCAACAGGAGATTCATTACTTTTTGAATAATAACTTCCAAC
TACCTACTGCTTTCACAAGCCTTTCTCATAAGCTTCAACTTCTGAATAAAGAGGGGGTTATAGATGAAAAGATTTTACGA
AAGTATACGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

58.514

99.691

0.583
  comGA Staphylococcus aureus N315

58.514

99.691

0.583