Detailed information    

insolico Bioinformatically predicted

Overview

Name   sepM   Type   Regulator
Locus tag   RFN65_RS12675 Genome accession   NZ_CP133703
Coordinates   2501595..2502620 (-) Length   341 a.a.
NCBI ID   WP_063335313.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   processing of CSP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2496595..2507620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS12645 (RFN65_12645) gerR 2497097..2497678 (-) 582 WP_015383592.1 sporulation specific transcriptional regulator GerR -
  RFN65_RS12650 (RFN65_12650) rpmF 2497824..2498003 (-) 180 WP_309529617.1 50S ribosomal protein L32 -
  RFN65_RS12655 (RFN65_12655) ylbN 2498025..2498543 (-) 519 WP_014479718.1 DUF177 domain-containing protein -
  RFN65_RS12660 (RFN65_12660) - 2498571..2498777 (+) 207 WP_306649529.1 hypothetical protein -
  RFN65_RS12665 (RFN65_12665) tmcAL 2498752..2499999 (+) 1248 WP_309529618.1 nucleotidyltransferase -
  RFN65_RS12670 (RFN65_12670) - 2500129..2501481 (-) 1353 WP_309529619.1 IS1182 family transposase -
  RFN65_RS12675 (RFN65_12675) sepM 2501595..2502620 (-) 1026 WP_063335313.1 protease DdcP Regulator
  RFN65_RS12680 (RFN65_12680) ylbK 2502622..2503404 (-) 783 WP_003232221.1 patatin family protein -
  RFN65_RS12685 (RFN65_12685) spoVV 2503585..2504811 (+) 1227 WP_015383588.1 dipicolinic acid transporter SpoVV -
  RFN65_RS12690 (RFN65_12690) coaD 2504822..2505307 (-) 486 WP_003245283.1 pantetheine-phosphate adenylyltransferase -
  RFN65_RS12695 (RFN65_12695) rsmD 2505312..2505866 (-) 555 WP_015483196.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  RFN65_RS12700 (RFN65_12700) ylbG 2506189..2506461 (-) 273 WP_009967171.1 YlbG family protein -
  RFN65_RS12705 (RFN65_12705) ylbF 2506516..2506965 (-) 450 WP_003221370.1 regulatory iron-sulfur-containing complex subunit RicF Regulator
  RFN65_RS12710 (RFN65_12710) ylbE 2507081..2507320 (-) 240 WP_003232231.1 YlbE-like family protein -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37594.18 Da        Isoelectric Point: 6.5347

>NTDB_id=800155 RFN65_RS12675 WP_063335313.1 2501595..2502620(-) (sepM) [Bacillus subtilis strain CP35]
MLRKKHFSWMLVILILIAVLSFIKLPYYITKPGEATELASLIKVEGGYPEKGSLSLMTVKVGPANPFTYVWAKMHPYYEI
VPDESIKEEGESDKEYMKRQLQMMKSSQENAVIAAYQKAGKKVSYSFNGIYASSVVENMPAKGKIEVGDKIISADGKNYQ
SAEKLIDYISSKKAGDKVTLKIEREEKEKRVTLTLKQFPDEPDRAGIGVSLYTDRNVKVEPDIDFEIENIGGPSAGLMMS
LEIYNQLTKPDETKGYDIAGTGTIDVDGKVGPIGGIDQKVVAADKAGKDIFFAPNQNGASNSDYKNAVKTAKDIDSGMKI
VPVDTMQDAIDYLNKLKAKST

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=800155 RFN65_RS12675 WP_063335313.1 2501595..2502620(-) (sepM) [Bacillus subtilis strain CP35]
TTGCTACGTAAAAAACATTTTAGCTGGATGCTCGTCATCCTCATACTGATCGCCGTTTTATCATTTATAAAACTTCCATA
CTATATTACAAAACCGGGAGAAGCGACAGAGCTTGCTTCATTGATAAAAGTGGAGGGAGGCTACCCGGAAAAAGGAAGCC
TGTCCTTAATGACTGTTAAAGTGGGACCGGCTAATCCGTTTACATATGTGTGGGCAAAGATGCACCCTTATTATGAAATT
GTTCCTGATGAGAGCATTAAGGAGGAAGGCGAGTCAGATAAGGAGTATATGAAAAGACAGCTCCAAATGATGAAAAGCTC
GCAAGAAAATGCGGTTATAGCCGCCTATCAAAAGGCAGGAAAAAAAGTCAGCTATTCCTTTAACGGCATATACGCAAGCT
CAGTTGTAGAAAACATGCCGGCAAAAGGGAAAATAGAAGTCGGTGATAAAATCATCAGTGCAGACGGCAAAAACTATCAG
TCTGCTGAAAAGCTGATTGATTATATCAGCAGCAAAAAAGCGGGAGATAAAGTCACGTTAAAGATCGAAAGAGAAGAAAA
AGAAAAGCGTGTCACCCTTACGTTAAAACAATTTCCTGATGAGCCGGATCGGGCAGGAATCGGAGTTTCACTTTATACAG
ACAGAAACGTGAAGGTAGAGCCTGATATTGATTTTGAAATCGAAAATATTGGCGGCCCGTCAGCAGGGTTAATGATGTCC
CTTGAAATCTATAATCAGCTGACAAAACCAGACGAAACAAAAGGCTACGATATTGCGGGAACAGGTACGATTGATGTTGA
CGGAAAAGTAGGCCCGATCGGCGGCATTGATCAAAAAGTCGTCGCCGCAGACAAAGCCGGAAAAGACATTTTCTTCGCGC
CAAATCAAAACGGCGCCAGCAATTCCGATTATAAGAACGCAGTAAAAACAGCAAAAGATATTGATTCAGGTATGAAGATT
GTGCCTGTTGATACCATGCAGGATGCGATTGATTATTTGAATAAGCTGAAAGCGAAAAGCACCTGA

Domains

Predicted by InterProScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(232-331)

Peptidase family M50 (Peptidase_M50)

(59-220)

PDZ domain (PDZ_2)

(128-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sepM Streptococcus mutans UA159

39.143

100

0.402