Detailed information NTG0800115

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: P3K65_RS06020
• Genome accession: NZ_CP119629
• Coordinates: 1177163..1180888 (+)
• Length: 1241 a.a.
• NCBI ID: WP_000572277.1
• Uniprot ID: -
• Organism: Bacillus paranthracis strain Bc006
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1172163..1185888

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P3K65_RS06005 (P3K65_06005)	-	1172321..1172914 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
P3K65_RS06010 (P3K65_06010)	lepB	1172971..1173534 (+)	564	WP_000751900.1	signal peptidase I	-
P3K65_RS06015 (P3K65_06015)	addB	1173651..1177166 (+)	3516	WP_000058549.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
P3K65_RS06020 (P3K65_06020)	addA	1177163..1180888 (+)	3726	WP_000572277.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
P3K65_RS06025 (P3K65_06025)	-	1180901..1181188 (+)	288	WP_000255721.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
P3K65_RS06030 (P3K65_06030)	gerPF	1181293..1181508 (-)	216	WP_001141566.1	spore germination protein GerPF	-
P3K65_RS06035 (P3K65_06035)	-	1181551..1181937 (-)	387	WP_000902335.1	spore germination protein GerPE	-
P3K65_RS06040 (P3K65_06040)	gerPD	1181953..1182147 (-)	195	WP_001052802.1	spore germination protein GerPD	-
P3K65_RS06045 (P3K65_06045)	gerPC	1182154..1182768 (-)	615	WP_001070747.1	spore germination protein GerPC	-
P3K65_RS06050 (P3K65_06050)	gerPB	1182836..1183042 (-)	207	WP_001012508.1	spore germination protein GerPB	-
P3K65_RS06055 (P3K65_06055)	gerPA	1183057..1183278 (-)	222	WP_001111188.1	spore germination protein GerPA	-
P3K65_RS06060 (P3K65_06060)	-	1183375..1183554 (-)	180	WP_000462840.1	aspartyl-phosphate phosphatase Spo0E family protein	-
P3K65_RS06065 (P3K65_06065)	-	1183799..1184701 (+)	903	WP_000945296.1	fumarylacetoacetate hydrolase family protein	-
P3K65_RS06070 (P3K65_06070)	-	1184738..1185022 (-)	285	WP_000926868.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142743.99 Da
• Isoelectric Point: 4.8375

>NTDB_id=800115 P3K65_RS06020 WP_000572277.1 1177163..1180888(+) (addA) [Bacillus paranthracis strain Bc006]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEEGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSVPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKIRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGQSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=800115 P3K65_RS06020 WP_000572277.1 1177163..1180888(+) (addA) [Bacillus paranthracis strain Bc006]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCAGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCATTAGAAAAAGTATTAATTGATGAGCCGGGCTCTCAGCACATTAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCCAATCAAACAGAAAATGAACTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGGATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCCTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGGAAAGCAACGGAACTCGCAATGCTTCCTGACGGTCCAGCGCCCCGTGTTGAAACACTGCAAGCGGATTTAGCTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCATGGACAAGCGTATATGAAGCGATGCAAAACGTATCGTGGCAAACATTAA
AGCGTATTAAGAAAAGCGATTATAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGTCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGCGAACAAAGTGAAGAAGGTGAAATGAAGCCATCAGCTGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTTATGGTAGGTGACGTGAAACAGTCGATATATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGCTTCACGCAAGAAGGATTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAGCAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAAGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCATGGGAAGAAAGGATCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAATGGTTCTACAACTTACTGCAAGGATGGCGTGAATTTGCGCGCCAACAGTCTCTTTCCGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACTT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTTCCAGTCGTATTTGTCGCTGGACTTGGTCGTCGCTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCGCAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CACAACTTGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTCTTATACGTAGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACAGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTGGATGCGAGAGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCATTATATAGGC
ACCGTGATAGTGAAATACTTCTTGAATTAGGACAAGGAAGTGTTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGTAACACGTTACTCGCGCCAGAACCGGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAGGCCGTTCCCCTGCAAAGTGAACGGAAAGAAGAGGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAAATACGTGCACCAATTAGGACACGTCCTCGTTTTATGGAGAAAAAGGGATTAACATATGCAGAGCGCGG
AACAGCAGTACATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGGGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTCGAGCAGGCGGAAGAAATTGCGATTGAAAAAGTAATTTCATTCTTTGATAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGCAGAGCGGGGAATCCATTCTTGTCCAAGGGGTTATTGACTGCATGATTGAAGAGGAAGATG
GTATTACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGTGGATTTGATCAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTCCAGCTTTCGTTATATGCAAAAGCGCTTGAGAAAAGCTTACAACACCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1061-1232)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.747	100	0.537