Detailed information    

insolico Bioinformatically predicted

Overview

Name   helD   Type   Machinery gene
Locus tag   RFN65_RS04020 Genome accession   NZ_CP133703
Coordinates   801875..804199 (+) Length   774 a.a.
NCBI ID   WP_309530089.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 796875..809199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS04000 (RFN65_04000) cadA 797569..799677 (+) 2109 WP_309530088.1 heavy metal translocating P-type ATPase -
  RFN65_RS04005 (RFN65_04005) bdbD 799797..800465 (+) 669 WP_003228414.1 disulfide bond formation protein BdbD -
  RFN65_RS04010 (RFN65_04010) bdbC 800470..800886 (+) 417 WP_015384637.1 disulfide oxidoreductase -
  RFN65_RS04015 (RFN65_04015) yvgT 801073..801681 (+) 609 WP_003219999.1 trimeric intracellular cation channel family protein -
  RFN65_RS04020 (RFN65_04020) helD 801875..804199 (+) 2325 WP_309530089.1 DNA helicase IV Machinery gene
  RFN65_RS04025 (RFN65_04025) cysJ 804370..806187 (+) 1818 WP_309530307.1 assimilatory sulfite reductase (NADPH) flavoprotein subunit -
  RFN65_RS04030 (RFN65_04030) cysI 806213..807928 (+) 1716 WP_038829347.1 assimilatory sulfite reductase (NADPH) hemoprotein subunit -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 89839.40 Da        Isoelectric Point: 6.3899

>NTDB_id=800090 RFN65_RS04020 WP_309530089.1 801875..804199(+) (helD) [Bacillus subtilis strain CP35]
MNQQDKEWKEEQSRIDEVLQELEKKERFLETSAGGLKHDIIGLRKSFWEDVKVNFDDAHEAIETMASIKQQAELLSDREH
NHRRMDQQLKRIHQLKKSPYFGRIDFIENGEEQAERIYIGLASCLDEKEEHFLIYDWRAPISSLYYNYSPGKAEYEVPGE
TIEGEMVLKRQFIIKNGDLKAMFNTDMTIGDEMLQEVLSHHSDTQMKNIVSTIQKEQNQIIRNEKSKVLIVQGAAGSGKT
SAALQRVAYLLYRHRGVIDAGQIVLFSPNFLFNSYVSSVLPELGEENMEQATFQEYIEHRLGRKFKCESPFDQLEYCLTE
TKGGDFPTRLAGITWKAGLSFQQFIDEYVTRLSSEGMIFKNIIFRGHKLITKEQIQSYFYSLDQNHSIPNRMEQTAKWLL
SELNKLEKKERRKDWVVHEAELLDKEDYLDVYKKLQERKRFSESTFNDYQREQQLLAAIIVKKAFKPLKQAVRLLAFLDV
SQLYLQLFSGLGGKFQHEKMAAIGELTRSAFTDNKLLYEDSAPFLYMQDLIEGRKKNTKIKHLFIDEAQDYSPFQMAYMR
SIFPAASMTVLGDINQSIYAHTINGDQRMDACFEDEPAEYVRLKRTYRSTRQIVEFTKAMLQDGVDIEPFNRSGEMPLVV
KTEGHESLCQKLAQEIGRLKKKGHETIAVICKTAQQCIQAHAHMSEYTDVRLIHKENQPFQKGVCVIPVYLAKGIEFDAV
LVYDASEEHYHTEHDRRLLYTACTRAMHMLAVFYTGEASPFVTAVPPHLYQMAE

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=800090 RFN65_RS04020 WP_309530089.1 801875..804199(+) (helD) [Bacillus subtilis strain CP35]
ATGAATCAGCAGGATAAGGAATGGAAGGAAGAGCAGTCGAGAATAGATGAGGTGCTGCAGGAGCTCGAAAAAAAAGAGCG
GTTTCTGGAAACGTCTGCGGGCGGGTTGAAGCATGATATCATCGGATTAAGAAAAAGCTTTTGGGAGGATGTTAAGGTTA
ATTTTGATGATGCCCATGAAGCGATTGAAACGATGGCAAGCATTAAGCAGCAGGCAGAGCTGCTGTCTGATCGGGAACAC
AATCATCGGCGCATGGATCAGCAGCTCAAACGAATTCATCAATTAAAAAAATCGCCTTATTTCGGCCGGATTGATTTTAT
TGAAAACGGCGAGGAGCAGGCAGAGCGAATTTATATCGGTTTAGCTTCCTGTTTGGATGAGAAGGAGGAACACTTTCTGA
TTTATGACTGGAGGGCGCCGATATCAAGTTTGTACTACAATTATTCCCCAGGAAAAGCGGAGTACGAGGTGCCCGGTGAA
ACGATAGAAGGGGAAATGGTGCTGAAACGCCAGTTTATAATCAAAAACGGCGATCTCAAGGCGATGTTTAATACCGATAT
GACGATTGGTGATGAAATGCTGCAAGAGGTATTAAGTCATCACTCCGATACCCAAATGAAAAACATCGTGTCTACGATTC
AGAAAGAACAAAATCAGATTATCCGTAATGAAAAAAGCAAGGTCCTGATCGTGCAGGGAGCGGCTGGCAGCGGGAAAACA
TCGGCTGCGCTTCAGCGTGTCGCTTATTTGTTATACAGGCACCGCGGCGTCATTGATGCCGGACAAATCGTTTTGTTTTC
TCCCAACTTCTTATTTAACAGCTATGTCTCCTCGGTTTTGCCTGAGCTAGGAGAAGAGAATATGGAGCAGGCGACGTTTC
AGGAATATATCGAGCATCGTCTTGGCCGCAAGTTCAAATGTGAAAGCCCCTTTGATCAGCTCGAATATTGCCTGACCGAA
ACAAAGGGAGGCGATTTTCCAACAAGGCTTGCGGGAATCACATGGAAGGCGGGTTTATCCTTTCAGCAATTCATTGATGA
ATACGTCACACGGCTTTCCTCTGAAGGGATGATTTTTAAAAATATCATCTTCCGCGGACACAAGCTGATCACAAAGGAGC
AAATTCAATCCTATTTTTACTCATTGGATCAAAATCACTCCATTCCGAATCGGATGGAGCAAACCGCGAAGTGGCTGCTC
AGTGAGCTTAACAAGCTTGAGAAAAAGGAACGGCGAAAGGATTGGGTTGTTCATGAAGCTGAGCTGCTTGACAAAGAGGA
CTACTTGGACGTGTACAAAAAACTGCAGGAAAGGAAACGGTTCAGCGAGAGTACGTTTAACGATTATCAAAGAGAGCAGC
AGCTGCTTGCCGCCATCATTGTGAAAAAAGCTTTCAAACCGCTCAAGCAGGCAGTCAGGCTCCTTGCATTTCTTGATGTT
TCACAGCTGTATTTGCAATTGTTTTCTGGCTTGGGAGGGAAATTTCAGCATGAAAAGATGGCCGCGATAGGTGAGCTGAC
CCGTTCGGCATTTACGGATAATAAATTGCTCTATGAGGACTCGGCACCGTTTCTTTATATGCAGGATCTGATTGAGGGCA
GAAAGAAAAATACGAAGATCAAACATCTATTTATTGACGAAGCCCAGGACTATTCACCGTTTCAGATGGCTTACATGAGA
AGCATTTTTCCGGCTGCCAGCATGACAGTGCTCGGTGATATCAATCAGTCCATCTACGCGCATACCATAAACGGGGATCA
GCGTATGGATGCGTGCTTTGAGGATGAGCCTGCTGAGTATGTTCGTTTAAAGAGAACATACAGATCAACAAGGCAGATTG
TCGAGTTTACCAAAGCGATGCTTCAAGATGGAGTGGATATTGAGCCGTTTAATAGAAGCGGGGAAATGCCGCTCGTTGTC
AAAACTGAAGGACATGAGAGCCTTTGCCAAAAGCTCGCGCAAGAAATTGGCCGGCTCAAGAAAAAAGGGCACGAAACAAT
AGCCGTCATCTGCAAGACTGCGCAGCAATGCATACAGGCGCACGCCCACATGAGTGAATATACAGATGTTCGCCTGATTC
ATAAAGAAAATCAACCATTTCAAAAGGGTGTTTGCGTGATTCCGGTGTATTTAGCGAAGGGAATTGAATTTGACGCAGTG
CTTGTCTATGATGCATCTGAAGAACACTATCATACAGAGCACGACCGCAGGCTTTTATATACTGCGTGCACCCGTGCGAT
GCACATGCTGGCGGTATTTTATACAGGTGAAGCCAGTCCGTTTGTAACCGCTGTGCCGCCTCACTTATATCAGATGGCTG
AATGA

Domains

Predicted by InterProScan.

AAA domain (AAA_19)

(217-580)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(704-751)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  helD Bacillus subtilis subsp. subtilis str. 168

98.32

100

0.983