Detailed information NTG0000644

Experimentally validated

Overview

• Name: helD
• Type: Machinery gene
• Locus tag: BSU_33450
• Genome accession: NC_000964
• Coordinates: 3434327..3436651 (-)
• Length: 774 a.a.
• NCBI ID: NP_391225.1
• Uniprot ID: O32215
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: homologous recombination Homologous recombination

Function

The B. subtilis DeltahelD allele rendered cells proficient in transformational recombination and moderately sensitive to methyl methanesulfonate when present in an otherwise Rec(+) strain.

Genomic Context

Location: 3429327..3441651

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_33430 (BSU33430)	cysI	3430598..3432313 (-)	1716	NP_391223.1	assimilatory sulfite reductase (hemoprotein beta-subunit)	-
BSU_33440 (BSU33440)	cysJ	3432339..3434156 (-)	1818	NP_391224.1	assimilatory sulfite reductase (flavoprotein alpha-subunit)	-
BSU_33450 (BSU33450)	helD	3434327..3436651 (-)	2325	NP_391225.1	transcription factor	Machinery gene
BSU_33460 (BSU33460)	yvgT	3436849..3437457 (-)	609	NP_391226.1	putative integral membrane protein	-
BSU_33470 (BSU33470)	bdbC	3437644..3438060 (-)	417	NP_391227.1	thiol-disulfide oxidoreductase	-
BSU_33480 (BSU33480)	bdbD	3438065..3438733 (-)	669	NP_391228.1	thiol-disulfide oxidoreductase	-
BSU_33490 (BSU33490)	cadA	3438853..3440961 (-)	2109	NP_391229.3	Cd(II), Zn(II) and Co(II) exporter (ATPase)	-

Sequence

Protein

• Length: 774 a.a.
• Molecular weight: 89925.54 Da
• Isoelectric Point: 6.5124

>NTDB_id=644 BSU_33450 NP_391225.1 3434327..3436651(-) (helD) [Bacillus subtilis subsp. subtilis str. 168]
MNQQDKEWKEEQSRIDEVLKELEKKERFLETSAGGLKHDIIGLRKSFWEDVKVNFDDAHEAIETMASIKQQAELLSDREH
NHRRMDQQLKRIHQLKKSPYFGRIDFIENGEEQAERIYIGLASCLDEKEEHFLIYDWRAPISSLYYNYSPGKAEYEVPGE
TIEGEMVLKRQFMIKNGTLKAMFNTDMTIGDEMLQEVLSHHSDTQMKNIVSTIQKEQNQIIRNEKSKILIVQGAAGSGKT
SAALQRVAYLLYRHRGVIDAGQIVLFSPNFLFNSYVSSVLPELGEENMEQATFQEYIEHRLGRKFKCESPFDQLEYCLTE
TKGGDFPTRLAGITWKAGLSFQQFINEYVTRLSSEGMIFKNIIFRGQKLITKEQIQSYFYSLDQNHSIPNRMEQTAKWLL
SELNKLEKKERRKDWVVHEAELLDKEDYLDVYKKLQERKRFSESTFNDYQREQQLLAAIIVKKAFKPLKQAVRLLAFLDV
TQLYLQLFSGWGGKFQHEKMDAIGELTRSAFTDNKLLYEDAAPFLYMQDLIEGRKKNTKIKHLFIDEAQDYSPFQMAYMR
SIFPAASMTVLGDINQSIYAHTINGDQRMDACFEDEPAEYVRLKRTYRSTRQIVEFTKAMLQDGADIEPFNRSGEMPLVV
KTEGHESLCQKLAQEIGRLKKKGHETIAVICKTAHQCIQAHAHMSEYTDVRLIHKENQPFQKGVCVIPVYLAKGIEFDAV
LVYDASEEHYHTEHDRRLLYTACTRAMHMLAVFYTGEASPFVTAVPPHLYQIAE

Nucleotide

• Length: 2325 bp

>NTDB_id=644 BSU_33450 NP_391225.1 3434327..3436651(-) (helD) [Bacillus subtilis subsp. subtilis str. 168]
ATGAATCAGCAGGATAAGGAATGGAAGGAAGAGCAGTCGAGAATAGATGAGGTGCTGAAGGAGCTCGAAAAAAAAGAGCG
GTTTCTGGAAACGTCTGCGGGCGGGTTGAAGCATGATATCATCGGATTAAGAAAAAGCTTTTGGGAGGATGTTAAGGTTA
ATTTTGATGATGCCCATGAAGCGATTGAAACGATGGCTAGCATTAAGCAGCAGGCAGAGCTGCTGTCTGATCGGGAACAC
AATCATCGGCGCATGGATCAGCAGCTCAAACGAATTCATCAATTGAAAAAATCGCCTTATTTCGGCCGGATTGATTTTAT
TGAAAACGGCGAGGAGCAGGCAGAGCGAATTTATATCGGTTTAGCTTCCTGTTTGGATGAGAAGGAGGAACACTTTCTGA
TCTATGACTGGAGGGCGCCGATATCAAGTTTGTACTACAATTATTCCCCAGGAAAAGCGGAGTACGAGGTGCCCGGTGAA
ACGATAGAAGGGGAAATGGTGCTGAAACGCCAGTTTATGATCAAAAACGGCACTCTCAAGGCGATGTTTAATACCGATAT
GACGATTGGCGATGAAATGCTGCAAGAGGTATTAAGCCATCACTCTGATACCCAAATGAAAAACATCGTGTCTACGATTC
AGAAAGAACAAAATCAGATTATCCGTAATGAAAAAAGCAAGATCCTGATCGTGCAGGGAGCGGCTGGCAGCGGGAAAACA
TCGGCTGCGCTTCAGCGTGTCGCTTATTTGTTATACAGGCACCGCGGTGTCATTGATGCCGGACAAATCGTTTTGTTTTC
ACCCAACTTCTTATTTAACAGCTATGTCTCCTCTGTTTTGCCGGAGTTAGGAGAAGAGAATATGGAGCAGGCGACGTTTC
AGGAATATATCGAGCATCGTCTTGGCCGCAAGTTCAAATGTGAAAGCCCCTTTGATCAGCTCGAATATTGCCTGACCGAA
ACAAAGGGAGGCGATTTTCCAACAAGGCTTGCAGGAATCACATGGAAGGCGGGTTTATCCTTTCAGCAATTCATTAATGA
ATACGTCACACGGCTTTCCTCTGAAGGGATGATTTTTAAAAATATTATATTCCGCGGACAAAAGCTGATCACAAAGGAGC
AAATTCAATCCTATTTTTACTCATTGGATCAAAATCACTCCATTCCGAATCGGATGGAGCAAACCGCGAAGTGGCTGCTG
AGTGAGCTTAACAAGCTTGAGAAAAAGGAACGGCGAAAGGATTGGGTTGTTCATGAAGCTGAGCTGCTTGACAAAGAGGA
CTACTTGGACGTGTACAAAAAACTGCAGGAAAGGAAACGGTTCAGCGAGAGTACGTTTAACGATTATCAAAGAGAGCAGC
AGCTGCTTGCCGCCATCATTGTGAAAAAAGCTTTCAAACCGCTCAAACAGGCAGTCAGGCTCCTTGCATTTCTTGATGTT
ACACAACTTTATTTGCAATTGTTTTCTGGCTGGGGAGGAAAATTTCAACATGAGAAGATGGATGCGATAGGTGAGCTGAC
CCGTTCGGCATTTACGGATAATAAATTGCTCTATGAGGACGCGGCACCGTTTCTTTATATGCAGGATCTGATTGAGGGCA
GAAAGAAAAATACGAAAATCAAACATCTATTTATTGACGAAGCCCAGGACTATTCACCGTTTCAGATGGCTTACATGAGA
AGCATTTTTCCGGCTGCCAGCATGACAGTGCTCGGTGATATCAATCAGTCCATCTACGCGCATACCATAAACGGGGATCA
GCGTATGGATGCGTGCTTTGAGGATGAGCCTGCTGAGTATGTTCGTTTAAAGAGAACATACAGATCAACAAGGCAGATTG
TCGAGTTTACCAAAGCGATGCTTCAAGATGGAGCGGATATTGAGCCGTTTAATAGAAGCGGGGAAATGCCGCTCGTTGTC
AAAACTGAAGGACATGAGAGCCTTTGCCAAAAGCTCGCGCAAGAAATTGGCCGGCTCAAGAAAAAAGGGCACGAAACAAT
AGCCGTCATCTGCAAGACTGCGCATCAATGCATACAGGCGCACGCCCACATGAGTGAATATACAGATGTTCGCCTGATTC
ATAAAGAAAATCAACCATTTCAAAAGGGTGTTTGCGTGATTCCGGTGTATTTAGCGAAGGGAATTGAATTTGACGCAGTG
CTTGTCTATGATGCATCTGAAGAACACTATCATACAGAGCACGACCGCAGGCTTTTATATACTGCGTGCACCCGTGCGAT
GCACATGCTGGCGGTATTTTATACAGGTGAAGCCAGTCCGTTTGTAACCGCTGTGCCGCCTCACTTATATCAGATTGCTG
AATGA

Domains

Predicted by InterProScan.

• AAA domain (AAA_19) (217-580)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (704-751)

3D structure

• PDB: 6VSX
• PDB: 6WVK
• PDB: 6ZCA
• PDB: 6ZFB
• PDB: 8XA8

References

• [1]: Tomáš Kovaľ et al. (2019) Domain structure of HelD, an interaction partner of Bacillus subtilis RNA polymerase. FEBS Letters 593(9):996-1005. [PMID: 30972737]
• [2]: Gundeep Kaur et al. (2018) Bacillus subtilis HelD, an RNA Polymerase Interacting Helicase, Forms Amyloid-Like Fibrils. Frontiers in Microbiology 9:1934. [PMID: 30186259]
• [3]: B Carrasco et al. (2001) Genetic recombination in Bacillus subtilis 168: effect of DeltahelD on DNA repair and homologous recombination. Journal of Bacteriology 183(19):5772-7. [PMID: 11544244]