Detailed information    

insolico Bioinformatically predicted

Overview

Name   helD   Type   Machinery gene
Locus tag   RFN60_RS16945 Genome accession   NZ_CP133653
Coordinates   3271395..3273719 (-) Length   774 a.a.
NCBI ID   WP_309520502.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP38     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3266395..3278719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN60_RS16935 (RFN60_16935) cysI 3267667..3269382 (-) 1716 WP_309520501.1 assimilatory sulfite reductase (NADPH) hemoprotein subunit -
  RFN60_RS16940 (RFN60_16940) cysJ 3269408..3271225 (-) 1818 WP_139451198.1 assimilatory sulfite reductase (NADPH) flavoprotein subunit -
  RFN60_RS16945 (RFN60_16945) helD 3271395..3273719 (-) 2325 WP_309520502.1 DNA helicase IV Machinery gene
  RFN60_RS16950 (RFN60_16950) - 3274298..3274732 (+) 435 WP_015251231.1 hypothetical protein -
  RFN60_RS16955 (RFN60_16955) yvgT 3274842..3275453 (-) 612 WP_048655203.1 trimeric intracellular cation channel family protein -
  RFN60_RS16960 (RFN60_16960) bdbC 3275640..3276056 (-) 417 WP_003228417.1 disulfide oxidoreductase -
  RFN60_RS16965 (RFN60_16965) bdbD 3276061..3276729 (-) 669 WP_003228414.1 disulfide bond formation protein BdbD -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 89954.51 Da        Isoelectric Point: 6.4799

>NTDB_id=799872 RFN60_RS16945 WP_309520502.1 3271395..3273719(-) (helD) [Bacillus subtilis strain CP38]
MNQQDKEWKEEQSRIDEVLQELEKKERFLETSAGGLKHDIIGLRKSFWEDVKVNFDDAHEAIETMASIKQQAELLSDREQ
NHRRMDQQLKRIHQLKKSPYFGRIDFIENGEEQAERIYIGLASCLDEKEEHFLIYDWRAPISSLYYNYSPGKAEYEVPGE
TIEGEMVLKRQFMIKNGTLKAMFNTDMTIGDEMLQEVLSHHSDTQMKNIVSTIQKEQNQIIRNEKSKILIVQGAAGSGKT
SAALQRVAYLLYRHRGVIDAGQIVLFSPNFLFNSYVSSVLPELGEENMEQATFQEYIEHRLGRKFKCESAFDQLEYCLTE
TKEGDFPTRLAGITWKAGLSFQQFINEYVTRLSSEGMIFKNIIFRGQKLITKEQIQSYFYSLDQNHSIPNRMEQTAKWLL
SELNKLEKKERRKDWVVHEAELLDKEDYLDVYKKLQERKRFSESTFNDYQREQQLLAAIIVKKAFKPLKQAVRLLAFLDV
KQLYLQLFSGWGGKFQDEKKAAIGELTRSAFTDNKLLYEDAAPFLYMQDLIEGRKKNTKIKHLFIDEAQDYSPFQMAYMR
SIFPAASMTVLGDINQSIYAHTIHGDQRMDACFEDEPAEYVRLKRTYRSTRQIVEFTKAMLQDGADIEPFNRSGEMPLVV
KTEGHESLCQKLAQEIGRLKKKGHETIAVICKTAQQCIQAHAHMSEYTDVRLIHKENQPFQKGVCVIPVYLAKGIEFDAV
LVYDASEEHYHTEHDRRLLYTACTRAMHMLAVFYTGEASPFVTAVPPRLYQNAE

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=799872 RFN60_RS16945 WP_309520502.1 3271395..3273719(-) (helD) [Bacillus subtilis strain CP38]
ATGAATCAGCAGGATAAGGAATGGAAGGAAGAGCAGTCGAGAATAGATGAGGTGCTGCAGGAGCTCGAAAAAAAAGAGCG
GTTTCTGGAAACGTCTGCGGGCGGGTTGAAGCATGATATCATCGGATTAAGAAAAAGCTTTTGGGAGGATGTTAAGGTTA
ATTTTGATGATGCCCATGAAGCGATTGAAACGATGGCAAGCATTAAGCAGCAGGCAGAGCTGCTGTCTGATCGGGAACAG
AATCATCGGCGCATGGATCAGCAGCTCAAACGAATTCATCAATTAAAAAAATCGCCTTATTTCGGCCGGATTGATTTTAT
TGAAAACGGCGAGGAGCAGGCAGAGCGAATTTATATCGGTTTAGCTTCCTGTTTGGATGAGAAGGAGGAACACTTTCTGA
TTTATGACTGGAGGGCGCCGATATCAAGTTTGTACTACAATTATTCTCCAGGAAAAGCGGAGTACGAGGTGCCCGGTGAA
ACGATAGAAGGGGAAATGGTGCTGAAACGCCAGTTTATGATCAAAAACGGCACTCTCAAGGCGATGTTTAATACCGATAT
GACGATTGGCGATGAAATGCTGCAAGAGGTATTAAGCCATCACTCCGATACCCAAATGAAAAACATCGTGTCTACGATTC
AGAAAGAACAAAATCAGATTATCCGTAATGAAAAAAGCAAGATCCTGATCGTGCAGGGAGCGGCTGGCAGCGGGAAAACA
TCGGCTGCGCTTCAGCGTGTCGCTTATTTGTTATACAGGCACCGCGGCGTCATTGATGCCGGACAAATCGTTTTGTTTTC
TCCCAACTTCTTATTTAACAGCTATGTCTCCTCTGTTTTGCCGGAGTTAGGAGAAGAGAATATGGAGCAGGCGACGTTTC
AGGAATATATCGAGCATCGTCTTGGCCGCAAGTTCAAATGTGAAAGCGCCTTTGATCAGCTCGAATATTGCCTGACCGAA
ACTAAGGAAGGCGATTTTCCAACAAGGCTCGCGGGAATCACATGGAAGGCGGGTTTATCCTTTCAGCAATTCATTAATGA
ATACGTCACACGGCTTTCCTCTGAAGGGATGATTTTTAAAAATATCATATTCCGCGGACAAAAGCTGATCACAAAGGAGC
AAATTCAATCCTATTTTTACTCATTGGATCAAAATCACTCCATTCCGAATCGGATGGAGCAAACCGCGAAGTGGCTGCTG
AGTGAGCTTAACAAGCTTGAGAAAAAGGAACGGCGAAAGGATTGGGTTGTTCATGAAGCTGAGCTGCTTGACAAAGAGGA
CTACTTGGACGTGTACAAAAAGCTGCAGGAAAGGAAACGGTTCAGCGAGAGTACGTTTAACGATTATCAAAGAGAGCAGC
AGCTGCTTGCCGCCATCATTGTGAAAAAAGCTTTCAAACCGCTCAAACAGGCGGTCAGACTCCTTGCATTTCTTGATGTG
AAACAGCTGTACCTGCAATTGTTTTCTGGCTGGGGAGGAAAATTTCAGGATGAGAAGAAGGCCGCGATAGGTGAACTGAC
CCGTTCGGCATTTACGGATAATAAATTGCTCTATGAGGACGCGGCACCGTTTCTTTATATGCAGGATCTGATTGAGGGCA
GAAAGAAAAATACGAAGATCAAACATCTATTTATTGACGAAGCCCAGGACTATTCACCGTTTCAGATGGCTTACATGAGA
AGCATTTTTCCGGCTGCCAGCATGACAGTGCTCGGTGATATCAATCAGTCCATCTACGCGCATACCATACACGGGGATCA
GCGTATGGATGCGTGCTTTGAGGATGAGCCTGCTGAGTATGTTCGTTTAAAGAGAACATACAGATCAACAAGGCAGATTG
TCGAGTTTACCAAAGCGATGCTTCAAGATGGAGCGGATATTGAGCCGTTTAATAGAAGCGGGGAAATGCCGCTCGTTGTC
AAAACTGAAGGACATGAGAGCCTTTGCCAAAAGCTCGCGCAAGAAATTGGCCGGCTCAAGAAAAAAGGGCACGAAACAAT
AGCCGTCATCTGCAAGACTGCGCAGCAATGCATACAGGCGCACGCCCACATGAGTGAATATACAGATGTTCGCCTGATTC
ATAAAGAAAATCAACCATTTCAAAAGGGTGTTTGCGTGATTCCGGTGTATTTAGCAAAGGGAATTGAATTTGACGCAGTG
CTTGTCTATGATGCATCTGAAGAACACTATCATACAGAGCACGACCGCAGGCTTTTATATACTGCGTGCACCCGTGCGAT
GCACATGCTGGCGGTATTTTATACAGGTGAAGCCAGTCCGTTTGTAACCGCTGTGCCGCCTCGCTTATATCAGAATGCTG
AATGA

Domains

Predicted by InterProScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(704-751)

AAA domain (AAA_19)

(217-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  helD Bacillus subtilis subsp. subtilis str. 168

98.45

100

0.985