Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RFN60_RS12785 Genome accession   NZ_CP133653
Coordinates   2492375..2492785 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain CP38     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2487375..2497785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN60_RS12755 (RFN60_12755) - 2487462..2487602 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  RFN60_RS12760 (RFN60_12760) yqeF 2487908..2488639 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  RFN60_RS12765 (RFN60_12765) cwlH 2488891..2489643 (-) 753 WP_015251672.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  RFN60_RS12770 (RFN60_12770) yqeD 2489830..2490456 (+) 627 WP_015251671.1 TVP38/TMEM64 family protein -
  RFN60_RS12775 (RFN60_12775) gnd 2490475..2491368 (-) 894 WP_015251670.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  RFN60_RS12780 (RFN60_12780) yqeB 2491620..2492342 (+) 723 WP_010886572.1 hypothetical protein -
  RFN60_RS12785 (RFN60_12785) nucA/comI 2492375..2492785 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  RFN60_RS12790 (RFN60_12790) - 2492981..2493331 (+) 351 Protein_2473 sigma-70 family RNA polymerase sigma factor -
  RFN60_RS12795 (RFN60_12795) spoIVCA 2493359..2494819 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  RFN60_RS12800 (RFN60_12800) - 2494777..2494955 (-) 179 Protein_2475 hypothetical protein -
  RFN60_RS12805 (RFN60_12805) arsC 2495310..2495729 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  RFN60_RS12810 (RFN60_12810) acr3 2495741..2496781 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  RFN60_RS12815 (RFN60_12815) arsK 2496804..2497244 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  RFN60_RS12820 (RFN60_12820) arsR 2497303..2497620 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=799846 RFN60_RS12785 WP_009967785.1 2492375..2492785(-) (nucA/comI) [Bacillus subtilis strain CP38]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=799846 RFN60_RS12785 WP_009967785.1 2492375..2492785(-) (nucA/comI) [Bacillus subtilis strain CP38]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCTGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGCTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGAGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTACGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529