Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   RFN60_RS06180 Genome accession   NZ_CP133653
Coordinates   1197742..1199571 (+) Length   609 a.a.
NCBI ID   WP_003245178.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP38     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1192742..1204571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN60_RS06145 (RFN60_06145) yjbC 1193414..1193992 (+) 579 WP_003244921.1 GNAT family N-acetyltransferase -
  RFN60_RS06150 (RFN60_06150) spx 1194173..1194568 (+) 396 WP_014476435.1 transcriptional regulator Spx -
  RFN60_RS06155 (RFN60_06155) yjbE 1194611..1195267 (-) 657 WP_015252355.1 TerC family protein -
  RFN60_RS06160 (RFN60_06160) - 1195437..1195577 (+) 141 WP_119122854.1 hypothetical protein -
  RFN60_RS06165 (RFN60_06165) mecA 1195544..1196200 (+) 657 WP_015252354.1 adaptor protein MecA Regulator
  RFN60_RS06170 (RFN60_06170) - 1196195..1196317 (-) 123 WP_003245684.1 hypothetical protein -
  RFN60_RS06175 (RFN60_06175) coiA 1196361..1197512 (+) 1152 WP_044052564.1 competence protein CoiA Machinery gene
  RFN60_RS06180 (RFN60_06180) pepF 1197742..1199571 (+) 1830 WP_003245178.1 oligoendopeptidase F Regulator
  RFN60_RS06185 (RFN60_06185) - 1199609..1199776 (-) 168 WP_003244944.1 hypothetical protein -
  RFN60_RS06190 (RFN60_06190) spxH 1200090..1200989 (-) 900 WP_003245184.1 protease adaptor protein SpxH -
  RFN60_RS06195 (RFN60_06195) bhbI 1200986..1201384 (-) 399 WP_003232928.1 group 2 truncated hemoglobin YjbI -
  RFN60_RS06200 (RFN60_06200) cwlQ 1201639..1202373 (-) 735 WP_309520547.1 bifunctional muramidase/murein lytic transglycosylase -
  RFN60_RS06205 (RFN60_06205) yjbK 1202388..1202960 (-) 573 WP_015252351.1 CYTH domain-containing protein -
  RFN60_RS06210 (RFN60_06210) - 1203085..1203453 (+) 369 WP_015252350.1 hypothetical protein -
  RFN60_RS06215 (RFN60_06215) yjbM 1203482..1204117 (+) 636 WP_003245294.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 70085.95 Da        Isoelectric Point: 5.1562

>NTDB_id=799797 RFN60_RS06180 WP_003245178.1 1197742..1199571(+) (pepF) [Bacillus subtilis strain CP38]
MAEEKKANQLPDRSEVKAEDTWRLEDIFPSDEAWNKEFQAVKELIPNLSKYKGKLADSADHLYEALTYQDKVMERLGRLY
TYAHMRSDQDTGNSFYQGLNDKAGNLYTQAASATAYLVPEILSIEEDKLQQFILEKEELKLYSHAIEEITKERPHVLSEK
EEALLAEASEVLGSSSNTFSVLNNADITFPSIKDEDGNEKQITHGNFINFLESENREVRKNAFDAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLVKTINKHLPLLHRYIALRKKVLELDEVHIYDLYTPLVKDAGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRNGAYSSGAYGTNPYILMNWHNNVNNLFTLVHEFGHS
VHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNNLEDEKQRLYILNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLTNVYYDLNKKYFGDGMVIDKEIGLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVDRYIDFL
KAGSSQYPIDVLKKAGVDMTSPEPIEAACKMFEEKLDEMEELLMKVKQS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=799797 RFN60_RS06180 WP_003245178.1 1197742..1199571(+) (pepF) [Bacillus subtilis strain CP38]
ATGGCTGAGGAAAAAAAAGCAAACCAACTGCCTGACAGAAGTGAGGTAAAGGCAGAAGACACATGGAGACTTGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCTGTAAAAGAATTAATTCCGAATTTATCTAAGTATAAAGGAA
AGCTGGCAGATTCAGCTGATCATTTATACGAGGCTCTTACGTATCAAGATAAAGTGATGGAGCGGCTGGGTAGGCTGTAC
ACATATGCGCATATGCGCTCTGACCAGGATACTGGGAACTCCTTTTACCAGGGGCTGAATGACAAGGCGGGAAACCTGTA
TACACAGGCCGCAAGCGCGACAGCTTATCTGGTTCCGGAGATTTTATCCATAGAAGAAGACAAACTGCAGCAGTTCATTC
TTGAAAAAGAAGAATTAAAGCTGTACTCTCATGCGATTGAGGAGATTACAAAGGAACGTCCGCATGTGCTGAGCGAGAAG
GAAGAGGCGCTGCTTGCTGAAGCCTCCGAGGTACTGGGGTCATCTTCAAATACATTCAGCGTGTTAAATAACGCAGATAT
TACGTTTCCGTCCATTAAAGACGAAGATGGGAATGAGAAACAGATCACTCACGGCAACTTTATTAATTTCTTGGAAAGTG
AAAACCGTGAAGTCCGCAAAAATGCGTTTGACGCAGTGTATAAAACGTACGGACAATATAAAAATACAATGGCCACGACG
CTTAGCGGCACTGTGAAAAAGGACAACTTCTACGCGAGAGTGAAAAAGTACAAGTCCGCGCGTGAGGCTGCGCTTTCTAA
CAACAGTATTCCTGAGGAAGTATACGATAACCTTGTTAAGACGATTAATAAGCATTTGCCGCTCCTGCACCGCTATATTG
CGCTAAGAAAGAAAGTGCTTGAACTTGATGAAGTGCATATTTATGACCTGTATACACCGCTTGTGAAAGATGCGGGGATG
AAGGTGACATACGAGGAAGCCAAAGATTACATGCTGAAAGGCCTCGCACCATTAGGGGAAGAATATGCCTCTATCCTAAA
AGAAGGCCTGGAAAACCGCTGGGTTGACGTTTACGAAAATAAAGGCAAACGCAATGGGGCTTATTCATCAGGAGCTTACG
GCACGAATCCGTATATTTTGATGAACTGGCATAATAACGTCAATAATCTCTTTACGCTTGTGCACGAGTTTGGACATTCC
GTACACAGCTACTATACGAGAAAGCACCAGCCTTATCCATACGGCAATTACAGCATCTTTGTCGCGGAAGTTGCCTCTAC
GACAAATGAAGCGCTCCTTGGCGAATATTTGCTGAATAATTTAGAGGATGAAAAACAGCGCTTATATATTCTCAACCATA
TGCTTGAGGGCTTCAGGGGAACGGTCTTCAGACAAACGATGTTCGCTGAATTTGAACATCTGATTCATACAAAGGCGCAA
GAAGGCGAGCCGCTTACACCTGAGCTTCTGACAAATGTCTATTACGACCTGAATAAAAAGTATTTTGGAGACGGCATGGT
GATTGATAAGGAAATCGGCCTTGAATGGTCGCGTATTCCGCACTTCTATTACAATTACTATGTGTATCAGTATGCGACAG
GGTACAGCGCTGCCCAAGCATTAAGCAGCCAGATTTTGAAGGAAGGAAAGCCGGCGGTTGACCGTTATATTGACTTCCTG
AAAGCGGGAAGCTCACAATACCCGATTGATGTCCTGAAAAAAGCGGGTGTTGATATGACGTCTCCAGAGCCAATCGAAGC
CGCGTGCAAAATGTTTGAAGAAAAACTTGATGAAATGGAAGAGCTGCTGATGAAAGTCAAGCAGTCTTAA

Domains

Predicted by InterProScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

48.822

97.537

0.476