Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CupriaWKF_RS01970 Genome accession   NZ_CP119572
Coordinates   407274..408752 (-) Length   492 a.a.
NCBI ID   WP_276099373.1    Uniprot ID   -
Organism   Cupriavidus sp. WKF15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 402274..413752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CupriaWKF_RS01935 (CupriaWKF_01935) - 402648..403310 (-) 663 WP_276099367.1 LON peptidase substrate-binding domain-containing protein -
  CupriaWKF_RS01940 (CupriaWKF_01940) rapZ 403425..404309 (-) 885 WP_276099368.1 RNase adapter RapZ -
  CupriaWKF_RS01945 (CupriaWKF_01945) - 404417..404788 (-) 372 WP_276099369.1 PsiF family protein -
  CupriaWKF_RS01950 (CupriaWKF_01950) - 404787..404945 (+) 159 WP_276099370.1 hypothetical protein -
  CupriaWKF_RS01955 (CupriaWKF_01955) hprK 404976..405947 (-) 972 WP_011296542.1 HPr(Ser) kinase/phosphatase -
  CupriaWKF_RS01960 (CupriaWKF_01960) - 406022..406477 (-) 456 WP_276099371.1 PTS sugar transporter subunit IIA -
  CupriaWKF_RS01965 (CupriaWKF_01965) raiA 406871..407224 (-) 354 WP_276099372.1 ribosome-associated translation inhibitor RaiA -
  CupriaWKF_RS01970 (CupriaWKF_01970) rpoN/rpoN1 407274..408752 (-) 1479 WP_276099373.1 RNA polymerase factor sigma-54 Machinery gene
  CupriaWKF_RS01975 (CupriaWKF_01975) lptB 408853..409638 (-) 786 WP_276100634.1 LPS export ABC transporter ATP-binding protein -
  CupriaWKF_RS01980 (CupriaWKF_01980) lptA 409683..410324 (-) 642 WP_276099374.1 lipopolysaccharide transport periplasmic protein LptA -
  CupriaWKF_RS01985 (CupriaWKF_01985) lptC 410412..411038 (-) 627 WP_276099375.1 LPS export ABC transporter periplasmic protein LptC -
  CupriaWKF_RS01990 (CupriaWKF_01990) - 411064..412047 (-) 984 WP_276099376.1 KpsF/GutQ family sugar-phosphate isomerase -
  CupriaWKF_RS01995 (CupriaWKF_01995) - 412046..412174 (+) 129 WP_276099377.1 hypothetical protein -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 54467.74 Da        Isoelectric Point: 4.9129

>NTDB_id=799690 CupriaWKF_RS01970 WP_276099373.1 407274..408752(-) (rpoN/rpoN1) [Cupriavidus sp. WKF15]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
GNGEANANGAGADEEGYSDSGGDDYGSDWSLDDFARRPQGDDDEKTPMQLRDAEPTLREHLMEQLAPLKISTRDKGLAIF
LIESLDDDGYLSASLEEICAELPEELEFEVEEVQAILALLQSFDPPGVGARNAAECLTLQLRRLQHPQRELALSIVNHHL
ELLAVRDYTRLKKALQVDEAALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKGGGGWIAQLNPDVMPRLRINDMYA
QILRGAKGESGAAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRV
TTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRSPLSDSRIAELLGEQGFVVARRTVAKYREA
LKIPAVNLRKTL

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=799690 CupriaWKF_RS01970 WP_276099373.1 407274..408752(-) (rpoN/rpoN1) [Cupriavidus sp. WKF15]
ATGAAACCGTCGCTACAGCTCCGCCTCTCCCAGCATCTTGCCCTCACGCCGCAATTGCAGCAGTCGATCCGGCTGCTGCA
GCTTTCCACGCTGGAACTGCAGCAGGAAGTCGAGCAGGCCCTGACCGAAAACCCGCTGCTCGAGCGCGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCTGCGGACGGCTCGGTCAATCTGCAGAGCGCACCGGCACCCGCACCAGCGGAACCGCAG
GGCAATGGCGAAGCAAACGCCAACGGCGCGGGCGCAGATGAAGAAGGCTACAGTGACAGCGGTGGCGACGATTACGGCAG
CGACTGGAGCCTGGACGATTTCGCGCGGCGCCCGCAGGGCGATGACGACGAGAAGACGCCCATGCAGTTGCGCGATGCCG
AGCCCACCCTGCGCGAACACCTGATGGAACAGCTCGCGCCGCTGAAGATCTCTACACGTGACAAGGGGCTCGCGATCTTC
CTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTAGAGGAGATCTGCGCCGAGCTGCCCGAGGAACTCGA
ATTCGAGGTCGAGGAAGTGCAGGCCATACTCGCGCTGCTGCAAAGCTTCGACCCGCCCGGCGTAGGCGCGCGCAATGCCG
CCGAATGCCTGACCCTGCAGCTGCGCCGGCTGCAGCACCCGCAGCGCGAGCTGGCATTGTCGATCGTCAACCATCACCTG
GAACTGCTTGCCGTGCGCGACTACACGCGGTTGAAAAAAGCACTGCAGGTGGACGAAGCGGCGCTCAAGGCCGCCCACGA
ATTGATCCGCTCGCTCGCGCCATATCCGGGCCACGCGTACAGCCGCCCCGAAGCCGACTTCGTGGTGCCGGACGTGTTCG
TGCGCAAGGGCGGCGGCGGATGGATCGCGCAGCTCAACCCGGACGTGATGCCGCGCCTGCGCATCAACGATATGTATGCG
CAGATCCTGCGCGGAGCCAAGGGAGAGTCCGGCGCGGCCGGCCTGCAGCAGAAGCTGCAGGAAGCGCGCTGGCTGATCAA
GAATATCCAGCAAAGGTTCGACACGATCCTGCGAGTTTCGCAGGCAATTGTCGAACGTCAGAAGAACTTTTTCACGCATG
GCGAAATCGCGATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGATTACACGAGTCAACCATATCCCGGGTG
ACGACTAATAAATACATGGCAACGCCAATGGGTACTTTCGAACTGAAGTACTTTTTCGGCAGCCACGTGTCCACCGAAAC
TGGTGGCGCGGCTTCATCAACGGCCATCCGCGCCTTGATCAAGCAACTTATAGGAGCCGAAGACCCGAGGAGTCCTCTTT
CCGACAGCCGAATTGCCGAATTGCTGGGTGAACAAGGCTTCGTCGTCGCGCGCCGTACCGTCGCCAAGTATCGCGAAGCC
CTGAAGATCCCTGCTGTCAATCTCCGCAAGACTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-490)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-319)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

80.4

100

0.817