Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RBG92_RS13095 Genome accession   NZ_CP133453
Coordinates   2643193..2643870 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain CREC8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2638193..2648870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBG92_RS13060 (RBG92_13060) gspG 2638673..2639110 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  RBG92_RS13065 (RBG92_13065) gspH 2639118..2639627 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  RBG92_RS13070 (RBG92_13070) gspI 2639624..2640001 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RBG92_RS13075 (RBG92_13075) gspJ 2639994..2640581 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  RBG92_RS13080 (RBG92_13080) gspK 2640574..2641557 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  RBG92_RS13085 (RBG92_13085) gspL 2641572..2642735 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  RBG92_RS13090 (RBG92_13090) gspM 2642732..2643193 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  RBG92_RS13095 (RBG92_13095) pilD 2643193..2643870 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  RBG92_RS13100 (RBG92_13100) bfr 2643899..2644375 (-) 477 WP_000675504.1 bacterioferritin -
  RBG92_RS13105 (RBG92_13105) bfd 2644447..2644641 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RBG92_RS13110 (RBG92_13110) chiA 2644810..2647503 (-) 2694 WP_106466481.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=799229 RBG92_RS13095 WP_000178161.1 2643193..2643870(+) (pilD) [Escherichia coli strain CREC8]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=799229 RBG92_RS13095 WP_000178161.1 2643193..2643870(+) (pilD) [Escherichia coli strain CREC8]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378