Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P0M30_RS00625 Genome accession   NZ_CP119425
Coordinates   128197..129570 (+) Length   457 a.a.
NCBI ID   WP_057194461.1    Uniprot ID   A0AAJ6A204
Organism   Limosilactobacillus fermentum strain KUFM408     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 123197..134570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P0M30_RS00595 (P0M30_00595) - 123793..124407 (-) 615 WP_259027332.1 sugar O-acetyltransferase -
  P0M30_RS00600 (P0M30_00600) rpiA 124435..125121 (-) 687 WP_259027799.1 ribose-5-phosphate isomerase RpiA -
  P0M30_RS00605 (P0M30_00605) tnpA 125156..125611 (-) 456 Protein_106 IS200/IS605 family transposase -
  P0M30_RS00610 (P0M30_00610) - 125680..126930 (+) 1251 WP_259027333.1 transposase -
  P0M30_RS00615 (P0M30_00615) - 127133..127444 (-) 312 WP_049182693.1 GNAT family N-acetyltransferase -
  P0M30_RS00620 (P0M30_00620) - 127645..128184 (+) 540 WP_057194477.1 dUTP diphosphatase -
  P0M30_RS00625 (P0M30_00625) radA 128197..129570 (+) 1374 WP_057194461.1 DNA repair protein RadA Machinery gene
  P0M30_RS00630 (P0M30_00630) - 129602..130735 (+) 1134 WP_259027334.1 PIN/TRAM domain-containing protein -
  P0M30_RS00635 (P0M30_00635) gltX 130806..132302 (+) 1497 WP_104877840.1 glutamate--tRNA ligase -
  P0M30_RS00640 (P0M30_00640) - 132370..132939 (+) 570 WP_127429171.1 TIGR01440 family protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49415.82 Da        Isoelectric Point: 8.7358

>NTDB_id=799207 P0M30_RS00625 WP_057194461.1 128197..129570(+) (radA) [Limosilactobacillus fermentum strain KUFM408]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTMLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLDVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGVLAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTDAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPPQDIEVVGVTTLGEALKISLG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=799207 P0M30_RS00625 WP_057194461.1 128197..129570(+) (radA) [Limosilactobacillus fermentum strain KUFM408]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTATAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTGCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGGAAGTCCACCAT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTACGGGCCCAACGCTTAGACGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGT
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACGATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGTCTTAGCCGGTCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGGGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGCGGTTTGACCGAGGTCGCAAACCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAACGCCCAAAGAACGGCGACGGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTTGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCGACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAGCGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGCCGCAAGACATCGAAGTCGTGGGGGTAACGACCCTTGGCGAAGCACTTAAAA
TTTCCCTTGGATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(64-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

67.991

99.125

0.674
  radA Streptococcus mitis NCTC 12261

67.77

99.125

0.672
  radA Streptococcus pneumoniae Rx1

67.77

99.125

0.672
  radA Streptococcus pneumoniae D39

67.77

99.125

0.672
  radA Streptococcus pneumoniae R6

67.77

99.125

0.672
  radA Streptococcus pneumoniae TIGR4

67.77

99.125

0.672
  radA Bacillus subtilis subsp. subtilis str. 168

63.676

100

0.637