Detailed information NTG0798545

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: RB265_RS16370
• Genome accession: NZ_CP133128
• Coordinates: 3167828..3168811 (-)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli O157:H7 strain PNUSAE146743
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 3162828..3173811

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RB265_RS16345 (RB265_16345)	bcsF	3162989..3163180 (+)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
RB265_RS16350 (RB265_16350)	bcsG	3163177..3164856 (+)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
RB265_RS16355 (RB265_16355)	ldrD	3164943..3165050 (-)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
RB265_RS16360 (RB265_16360)	yhjV	3165526..3166797 (+)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
RB265_RS16365 (RB265_16365)	dppF	3166827..3167831 (-)	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
RB265_RS16370 (RB265_16370)	amiE	3167828..3168811 (-)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
RB265_RS16375 (RB265_16375)	dppC	3168822..3169724 (-)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
RB265_RS16380 (RB265_16380)	dppB	3169734..3170753 (-)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
RB265_RS16385 (RB265_16385)	dppA	3170904..3172511 (-)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=798545 RB265_RS16370 WP_001196486.1 3167828..3168811(-) (amiE) [Escherichia coli O157:H7 strain PNUSAE146743]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=798545 RB265_RS16370 WP_001196486.1 3167828..3168811(-) (amiE) [Escherichia coli O157:H7 strain PNUSAE146743]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404