Detailed information NTG0798428

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: RB262_RS01115
• Genome accession: NZ_CP133126
• Coordinates: 223612..224595 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli O157:H7 strain PNUSAE146131
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 218612..229595

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RB262_RS01100 (RB262_01100)	dppA	219912..221519 (+)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-
RB262_RS01105 (RB262_01105)	dppB	221670..222689 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
RB262_RS01110 (RB262_01110)	dppC	222699..223601 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
RB262_RS01115 (RB262_01115)	amiE	223612..224595 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
RB262_RS01120 (RB262_01120)	dppF	224592..225596 (+)	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
RB262_RS01125 (RB262_01125)	yhjV	225626..226897 (-)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
RB262_RS01130 (RB262_01130)	ldrD	227373..227480 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
RB262_RS01135 (RB262_01135)	bcsG	227567..229246 (-)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
RB262_RS01140 (RB262_01140)	bcsF	229243..229434 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=798428 RB262_RS01115 WP_001196486.1 223612..224595(+) (amiE) [Escherichia coli O157:H7 strain PNUSAE146131]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=798428 RB262_RS01115 WP_001196486.1 223612..224595(+) (amiE) [Escherichia coli O157:H7 strain PNUSAE146131]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404