Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PY247_RS02330 Genome accession   NZ_CP119223
Coordinates   529307..530113 (-) Length   268 a.a.
NCBI ID   WP_004652529.1    Uniprot ID   A0A2N0WIQ9
Organism   Acinetobacter proteolyticus strain AP54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 524307..535113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY247_RS02310 (PY247_02310) - 525365..526063 (-) 699 WP_275573590.1 tetratricopeptide repeat protein -
  PY247_RS02315 (PY247_02315) hisS 526093..527387 (-) 1295 Protein_456 histidine--tRNA ligase -
  PY247_RS02320 (PY247_02320) ispG 527384..528499 (-) 1116 WP_004652525.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  PY247_RS02325 (PY247_02325) - 528522..529316 (-) 795 WP_275573591.1 helix-turn-helix domain-containing protein -
  PY247_RS02330 (PY247_02330) pilF 529307..530113 (-) 807 WP_004652529.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  PY247_RS02335 (PY247_02335) rlmN 530131..531365 (-) 1235 Protein_460 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  PY247_RS02340 (PY247_02340) ndk 531487..531918 (-) 432 WP_004652531.1 nucleoside-diphosphate kinase -
  PY247_RS02345 (PY247_02345) iscX 532026..532226 (-) 201 WP_004652532.1 Fe-S cluster assembly protein IscX -
  PY247_RS02350 (PY247_02350) - 532323..532874 (-) 552 WP_159724080.1 phosphatase PAP2 family protein -
  PY247_RS02355 (PY247_02355) - 532913..534184 (-) 1272 WP_275574132.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 268 a.a.        Molecular weight: 30458.43 Da        Isoelectric Point: 9.7018

>NTDB_id=798204 PY247_RS02330 WP_004652529.1 529307..530113(-) (pilF) [Acinetobacter proteolyticus strain AP54]
MNTTRPKTIIATAVVFTLLGLTACQSTSSTLKKDPEKAVQVRTQLAAEYIRNRDLDAAKRSLDQALKVDSRDANANMMMG
ILLQQEGSKTNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQQQRYNDAIEQFTRAGATLGYEQRFQSLENLGRIYLKLG
DVANAEKSFKQALQANRNATISMLELAEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGLRIARANADQLGMQVLV
NQLRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 807 bp        

>NTDB_id=798204 PY247_RS02330 WP_004652529.1 529307..530113(-) (pilF) [Acinetobacter proteolyticus strain AP54]
TTGAATACCACTCGACCAAAAACGATTATTGCGACAGCTGTTGTGTTTACTTTACTTGGCCTGACAGCATGTCAATCAAC
ATCTTCAACATTAAAAAAAGATCCAGAAAAAGCAGTGCAAGTCCGTACCCAACTTGCAGCAGAGTATATTCGTAATCGTG
ATTTAGACGCAGCCAAGCGTTCGCTTGACCAAGCCTTAAAAGTTGATTCACGGGATGCCAATGCCAACATGATGATGGGC
ATCTTACTGCAACAAGAGGGCAGCAAGACCAATATGGAAAAAGCTGAAGCTTATTTTAAGCGGGCAATTTCCTCAGAACC
TGATAATGCTCAAGCGCGTAATAATTACGGTACATATTTGTATCAACAGCAACGCTATAATGACGCTATTGAGCAATTTA
CTCGTGCGGGTGCAACCTTAGGCTATGAACAACGGTTTCAGTCGTTGGAAAACCTAGGACGAATCTATTTGAAATTGGGC
GATGTTGCGAATGCTGAAAAATCATTCAAACAGGCGCTACAAGCGAATCGAAACGCAACTATTTCAATGTTAGAGTTGGC
GGAAATTTTCTATTTGCAACAAAATAATAGAGATGCGTCGCAACTATACGAACAATATGTTCGTAACGTGGGACAGAAAA
ACCAAGGTGCTCGGGCGCTTTGGATTGGTCTACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTGTTAGTC
AATCAACTGCGCGCACTCTTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATG
GAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(45-80)

Tetratricopeptide repeat (TPR_16)

(151-209)

Tetratricopeptide repeat (TPR_16)

(91-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N0WIQ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.851

100

0.799
  pilF Acinetobacter baylyi ADP1

68.702

97.761

0.672