Detailed information NTG0079774

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: L6477_RS25595
• Genome accession: NZ_AP022886
• Coordinates: 4919426..4920775 (-)
• Length: 449 a.a.
• NCBI ID: WP_061531556.1
• Uniprot ID: -
• Organism: Bacillus cereus strain MRY14-0074
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4914426..4925775

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6477_RS25575 (BCM0074_5062)	secA	4914748..4917255 (-)	2508	WP_000579383.1	preprotein translocase subunit SecA	-
L6477_RS25580 (BCM0074_5063)	raiA	4917532..4918074 (-)	543	WP_000671189.1	ribosome-associated translation inhibitor RaiA	-
L6477_RS25585 (BCM0074_5064)	cspC	4918398..4918595 (-)	198	WP_001990088.1	cold shock protein CspC	-
L6477_RS25590 (BCM0074_5065)	comFC	4918722..4919426 (-)	705	WP_236797362.1	comF operon protein ComFC	-
L6477_RS25595 (BCM0074_5066)	comFA	4919426..4920775 (-)	1350	WP_061531556.1	ATP-dependent helicase ComFA	Machinery gene
L6477_RS25600 (BCM0074_5067)	-	4920903..4922213 (-)	1311	WP_000734330.1	C40 family peptidase	-
L6477_RS25605 (BCM0074_5068)	-	4922467..4922781 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
L6477_RS25610 (BCM0074_5069)	-	4922955..4923797 (-)	843	WP_000844766.1	DegV family protein	-
L6477_RS25615 (BCM0074_5070)	-	4924037..4924672 (+)	636	WP_000926685.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51370.06 Da
• Isoelectric Point: 9.7210

>NTDB_id=79774 L6477_RS25595 WP_061531556.1 4919426..4920775(-) (comFA) [Bacillus cereus strain MRY14-0074]
MVAGKQLLLEELSSDLRRELSDLKKKGEIICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGTLSTGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKEERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KERAARIYLTATPDEKWKRNFRRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEMSLLLKGLDHRIDGVHAEDPMRKEKVAAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=79774 L6477_RS25595 WP_061531556.1 4919426..4920775(-) (comFA) [Bacillus cereus strain MRY14-0074]
ATGGTTGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAAGGG
AGAGATCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGAATTCATGAAAGAAAAGGAGAAAGAGAATTACATTCGTTACAGTGGAAAGGAACTTT
GTCTACTGGTCAGGAGTTGGCAGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCGTTTTTTATTTGGGCGGTAT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGAAGAAAGAGTTTGTATTGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCATTCC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAACAAGCGATG
AAAGAGAGAGCGGCGCGTATTTATTTAACTGCAACCCCGGATGAAAAGTGGAAGCGCAATTTCAGAAGGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGTAATTGGAAGAAAA
GCCTTCATCATAAAAAAATTCCTCGAGTGTTACTACAATGGTTGAAAATGTACGTAAACAAAAAATATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATGAGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAGGAAAAAGTGGCAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCACTACGGCAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

• Type III restriction enzyme, res subunit (ResIII) (107-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.914	93.096	0.483
comFA	Latilactobacillus sakei subsp. sakei 23K	40.5	89.087	0.361