Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PX653_RS24695 Genome accession   NZ_CP119083
Coordinates   5537802..5539271 (+) Length   489 a.a.
NCBI ID   WP_277415293.1    Uniprot ID   -
Organism   Pseudoduganella chitinolytica strain ACM 3522     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5532802..5544271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PX653_RS24670 (PX653_24670) - 5534278..5535276 (+) 999 WP_277415289.1 SIS domain-containing protein -
  PX653_RS24675 (PX653_24675) - 5535276..5535815 (+) 540 WP_277415290.1 HAD family hydrolase -
  PX653_RS24680 (PX653_24680) lptC 5535828..5536430 (+) 603 WP_277415291.1 LPS export ABC transporter periplasmic protein LptC -
  PX653_RS24685 (PX653_24685) lptA 5536440..5537000 (+) 561 WP_277415292.1 lipopolysaccharide transport periplasmic protein LptA -
  PX653_RS24690 (PX653_24690) lptB 5537017..5537796 (+) 780 WP_277418653.1 LPS export ABC transporter ATP-binding protein -
  PX653_RS24695 (PX653_24695) rpoN/rpoN1 5537802..5539271 (+) 1470 WP_277415293.1 RNA polymerase factor sigma-54 Machinery gene
  PX653_RS24700 (PX653_24700) hpf 5539356..5539706 (+) 351 WP_277415294.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PX653_RS24705 (PX653_24705) - 5541024..5542103 (+) 1080 WP_277415295.1 fatty acid desaturase -
  PX653_RS24710 (PX653_24710) - 5542188..5543192 (+) 1005 WP_277415296.1 aromatic ring-hydroxylating dioxygenase subunit alpha -
  PX653_RS24715 (PX653_24715) - 5543234..5544055 (+) 822 WP_277415297.1 hypothetical protein -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 54351.95 Da        Isoelectric Point: 5.1204

>NTDB_id=797601 PX653_RS24695 WP_277415293.1 5537802..5539271(+) (rpoN/rpoN1) [Pseudoduganella chitinolytica strain ACM 3522]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLTDNPLLERLDDPLDRSLRLLADGAIGQQSAPEAPAEGPP
PQADAPAPAEAEPYEGGEGDSGPEGDMDWSDAGRNKAPEDDDGRPQLEAGHRSLREHLMEQMRVTVQQPRDRALVELIVD
ALDENGYLEESLEEIHGRLPEELEVEMEELRTALALLQSFDPVGVGARNASECLALQIRKMPGVPLVTRRMALCIVEKHL
AWFAQRDFNKLKKALDCDDEDLREAQAVIRQCNPHPGAAFASDVSDYVVPDVIVKKGRNGWVVTLNNDVMPRLRVNALYA
SLLKQGKGESQMGAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTISRVTTQ
KYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQFTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYREALKI
PPVSLRKSL

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=797601 PX653_RS24695 WP_277415293.1 5537802..5539271(+) (rpoN/rpoN1) [Pseudoduganella chitinolytica strain ACM 3522]
ATGAAACAGTCTCTGCAACTGCGAACGTCGCAGCACCTCGCGCTGACCCCGCAGCTGCAACAGTCGATCCGGCTGCTGCA
GCTCTCCACGCTGGAGCTGCATCAGGAACTGGAACAGCTGCTGACGGATAACCCGCTGCTGGAACGGCTCGACGATCCGC
TCGACCGCTCGCTGCGGCTGCTGGCCGATGGCGCCATCGGCCAGCAAAGCGCGCCCGAGGCACCGGCCGAAGGGCCGCCG
CCGCAGGCGGATGCGCCGGCCCCCGCGGAAGCCGAGCCGTATGAAGGGGGCGAGGGCGACAGTGGCCCGGAAGGCGACAT
GGACTGGAGCGACGCCGGCCGCAACAAGGCGCCGGAAGACGACGACGGCCGGCCCCAGCTGGAAGCGGGCCACCGTTCGC
TGCGCGAACACCTGATGGAACAGATGCGGGTGACGGTGCAGCAGCCGCGCGACCGCGCCCTGGTGGAGCTGATCGTCGAT
GCGCTGGACGAGAACGGCTACCTGGAAGAAAGCCTGGAAGAGATCCACGGCCGCCTGCCGGAAGAGCTGGAAGTGGAAAT
GGAAGAGCTGCGTACGGCGCTGGCGCTGCTGCAGAGCTTCGATCCGGTCGGCGTGGGCGCGCGCAATGCATCCGAATGCC
TGGCGCTGCAGATCCGCAAGATGCCCGGCGTGCCCCTGGTGACGCGGCGCATGGCGCTGTGCATCGTCGAGAAGCACCTG
GCGTGGTTTGCCCAGCGCGACTTCAACAAGCTCAAGAAGGCTCTGGATTGCGACGACGAAGACCTGCGCGAGGCGCAGGC
GGTGATTCGCCAGTGCAATCCGCACCCGGGCGCCGCCTTTGCGTCCGACGTGTCAGACTACGTCGTACCGGATGTGATTG
TGAAGAAGGGGCGCAATGGCTGGGTCGTTACGTTGAACAACGACGTGATGCCGCGGCTGCGGGTGAACGCGTTGTATGCG
AGCCTGCTCAAGCAGGGCAAGGGCGAGTCGCAGATGGGCGCGCAACTGCAGGAAGCGAAGTGGCTGATCAAGAACATGCG
CCAGCGCTTCGACACGATCCTCCGTGTTGCCCAGGCCATCGTCGAGCGCCAGAAGAACTTTTTCTCGCACGGTGCGGTGG
CAATGCGGCCCCTTGTGTTGCGTGAAATAGCTGATACACTGGGGCTACACGAGAGCACGATCTCTCGGGTGACAACTCAA
AAATATATGCTGACCCCGCATGGCATGTTTGAGTTGAAGTACTTTTTTGGCAGCCACGTCGCAACCGAAGCTGGGGGAGA
AGCTTCCTCGACTGCGATCCGTGCGCTGATCGTGCAATTCACAGGAGCCGAAGACCCCAAGAACCCTTTATCCGACAGTA
AGATTGCGGACATGCTGGGAGAACAGGGCATGGTGATTGCGCGACGGACTGTTGCCAAGTATCGCGAAGCCTTGAAAATC
CCTCCCGTCAGCCTCCGCAAGTCCTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(333-487)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-319)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.87

100

0.63