Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RA292_RS12375 Genome accession   NZ_CP132912
Coordinates   2425888..2426298 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. natto strain BN-P15-11-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2420888..2431298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA292_RS12345 - 2420975..2421115 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  RA292_RS12350 yqeF 2421421..2422152 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  RA292_RS12355 cwlH 2422404..2423156 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  RA292_RS12360 yqeD 2423343..2423969 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  RA292_RS12365 gnd 2423988..2424881 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  RA292_RS12370 yqeB 2425133..2425855 (+) 723 WP_010886572.1 hypothetical protein -
  RA292_RS12375 nucA/comI 2425888..2426298 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  RA292_RS12380 - 2426494..2426844 (+) 351 Protein_2414 sigma-70 family RNA polymerase sigma factor -
  RA292_RS12385 spoIVCA 2426872..2428332 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  RA292_RS12390 - 2428290..2428468 (-) 179 Protein_2416 hypothetical protein -
  RA292_RS12395 arsC 2428823..2429242 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  RA292_RS12400 acr3 2429254..2430294 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  RA292_RS12405 arsK 2430317..2430757 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  RA292_RS12410 arsR 2430818..2431135 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=797226 RA292_RS12375 WP_009967785.1 2425888..2426298(-) (nucA/comI) [Bacillus subtilis subsp. natto strain BN-P15-11-1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=797226 RA292_RS12375 WP_009967785.1 2425888..2426298(-) (nucA/comI) [Bacillus subtilis subsp. natto strain BN-P15-11-1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529