Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PYW44_RS11725 Genome accession   NZ_CP118982
Coordinates   2400362..2401738 (-) Length   458 a.a.
NCBI ID   WP_002508585.1    Uniprot ID   A0A1E5THJ8
Organism   Staphylococcus equorum strain DSM 20674     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2395362..2406738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW44_RS11710 cysE 2396336..2396977 (-) 642 WP_031266086.1 serine O-acetyltransferase -
  PYW44_RS11715 gltX 2397324..2398781 (-) 1458 WP_069802619.1 glutamate--tRNA ligase -
  PYW44_RS11720 - 2399230..2400288 (-) 1059 WP_021339719.1 PIN/TRAM domain-containing protein -
  PYW44_RS11725 radA 2400362..2401738 (-) 1377 WP_002508585.1 DNA repair protein RadA Machinery gene
  PYW44_RS11730 - 2402083..2404545 (-) 2463 WP_002511351.1 ATP-dependent Clp protease ATP-binding subunit -
  PYW44_RS11735 - 2404557..2405567 (-) 1011 WP_021339720.1 protein arginine kinase -
  PYW44_RS11740 - 2405560..2406111 (-) 552 WP_002511349.1 UvrB/UvrC motif-containing protein -
  PYW44_RS11745 - 2406128..2406589 (-) 462 WP_021339721.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50617.15 Da        Isoelectric Point: 7.2148

>NTDB_id=797158 PYW44_RS11725 WP_002508585.1 2400362..2401738(-) (radA) [Staphylococcus equorum strain DSM 20674]
MAKKKVVFECMACGYQSAKWMGKCPNCGGWNQMEEIVEQKAASPKHGVRSRDRDNVSKVQKLNEIKQETTPRVKTKSDEF
NRVLGGGIVNGSLVLIGGDPGIGKSTLLLQICAALSQSHNVLYITGEESLNQTKLRAERLDEDSSELNVFAETDLEVIHE
TVKQLKPDLLVVDSIQTIFHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFE
GDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVKNPSEMFLEERSTNVPGSTIVSTMEGTRPLLIEVQALVTPTTFN
NPRRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLGIIVATASSFKDLTVDGLDCFIGEVGLTG
EVRRVSRIEQRVQEAEKLGFKRIIIPESNIGGWQFPSDIKVIGVKSVHEALKYALTKQ

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=797158 PYW44_RS11725 WP_002508585.1 2400362..2401738(-) (radA) [Staphylococcus equorum strain DSM 20674]
GTGGCCAAGAAGAAAGTTGTTTTTGAATGTATGGCATGTGGTTATCAGTCCGCAAAATGGATGGGCAAATGTCCAAATTG
TGGTGGCTGGAACCAAATGGAAGAAATTGTTGAGCAAAAGGCGGCAAGTCCAAAACATGGCGTGCGCAGTCGTGATCGCG
ACAACGTTTCAAAAGTACAGAAGTTAAATGAAATTAAACAAGAAACGACACCACGTGTTAAAACCAAGTCAGACGAATTT
AATCGTGTGCTCGGCGGAGGTATCGTTAATGGTTCGCTCGTATTGATTGGTGGAGACCCTGGTATAGGGAAATCAACATT
ACTATTACAAATATGTGCGGCACTATCACAATCGCATAACGTCTTATACATAACAGGTGAGGAATCATTAAATCAAACTA
AGCTAAGAGCTGAAAGATTGGATGAAGATTCAAGCGAACTTAATGTATTTGCAGAAACTGATTTAGAAGTCATTCACGAA
ACAGTAAAACAATTAAAACCGGATTTACTTGTAGTTGACTCAATCCAAACGATATTCCATCCGGAAATTAGTTCAGCACC
AGGCTCAGTGTCTCAAGTAAGAGAAAGTACACAAAGCTTAATGAACATTGCTAAACAAATGAATATTGCAACATTTATTG
TGGGACATGTAACAAAAGAAGGTCAAATTGCTGGTCCACGTTTATTAGAACACATGGTAGATACAGTACTTTATTTCGAA
GGTGACGAACACCATGCATATCGTATTTTGAGAGCGGTGAAAAACCGTTTTGGTTCTACAAATGAAATGGGTATTTTTGA
AATGAAACAAAGCGGATTGAAAGGTGTTAAAAACCCATCCGAGATGTTTTTAGAAGAACGTTCGACAAATGTGCCTGGCT
CGACTATCGTCTCTACAATGGAAGGCACGAGACCCTTACTCATAGAAGTTCAAGCTCTTGTTACGCCAACGACATTTAAT
AATCCTAGACGGATGGCAACGGGTATTGATCACAATCGTTTGAGTCTGTTGATGGCAGTCTTGGAGAAAAAAGAAAATTA
TTTACTGCAACAACAAGATGCGTATATTAAAGTTGCAGGTGGTGTAAGATTAACAGAACCTGCTGTTGATTTAGGTATCA
TTGTGGCAACTGCTTCAAGCTTTAAAGATCTAACAGTAGATGGTTTAGATTGCTTTATAGGAGAAGTTGGACTGACTGGA
GAAGTGAGACGCGTATCTCGTATAGAGCAACGTGTACAGGAAGCAGAGAAACTCGGATTTAAACGTATTATTATTCCAGA
ATCCAATATTGGCGGCTGGCAATTCCCTAGTGATATTAAGGTCATTGGCGTGAAAAGTGTTCACGAAGCCTTAAAATACG
CTTTGACAAAACAATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(67-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E5THJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

64.912

99.563

0.646
  radA Streptococcus pneumoniae Rx1

59.292

98.69

0.585
  radA Streptococcus pneumoniae D39

59.292

98.69

0.585
  radA Streptococcus pneumoniae R6

59.292

98.69

0.585
  radA Streptococcus pneumoniae TIGR4

59.292

98.69

0.585
  radA Streptococcus mitis SK321

59.292

98.69

0.585
  radA Streptococcus mitis NCTC 12261

59.292

98.69

0.585