Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   L6481_RS20335 Genome accession   NZ_AP022877
Coordinates   3950955..3951998 (-) Length   347 a.a.
NCBI ID   WP_001013200.1    Uniprot ID   A0AA96SWA8
Organism   Bacillus cereus strain MRY14-0060     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3945955..3956998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6481_RS20290 (BCM0060_4008) - 3946382..3946582 (-) 201 WP_000106084.1 YqzE family protein -
  L6481_RS20295 (BCM0060_4009) aroK 3946621..3947118 (-) 498 WP_000836630.1 shikimate kinase AroK -
  L6481_RS20300 (BCM0060_4010) - 3947239..3947889 (-) 651 WP_000183851.1 2OG-Fe(II) oxygenase -
  L6481_RS20305 (BCM0060_4011) comGG 3948065..3948436 (-) 372 WP_001231191.1 competence type IV pilus minor pilin ComGG -
  L6481_RS20310 (BCM0060_4012) comGF 3948433..3948903 (-) 471 WP_000923089.1 competence type IV pilus minor pilin ComGF -
  L6481_RS20315 (BCM0060_4013) comGE 3948873..3949175 (-) 303 WP_000829458.1 competence type IV pilus minor pilin ComGE -
  L6481_RS20320 (BCM0060_4014) comGD 3949168..3949623 (-) 456 WP_000810393.1 comG operon protein ComGD -
  L6481_RS20325 (BCM0060_4015) comGC 3949620..3949919 (-) 300 WP_001178696.1 comG operon protein ComGC -
  L6481_RS20330 (BCM0060_4016) comGB 3949931..3950962 (-) 1032 WP_000471911.1 comG operon protein ComGB -
  L6481_RS20335 (BCM0060_4017) comGA 3950955..3951998 (-) 1044 WP_001013200.1 competence protein ComGA Machinery gene
  L6481_RS20340 (BCM0060_4018) - 3952204..3952899 (+) 696 WP_000434117.1 helix-turn-helix domain-containing protein -
  L6481_RS20345 (BCM0060_4019) - 3953025..3953267 (+) 243 WP_000440711.1 DUF2626 domain-containing protein -
  L6481_RS20350 (BCM0060_4020) - 3953375..3954769 (+) 1395 WP_001094329.1 L-cystine transporter -
  L6481_RS20355 (BCM0060_4021) - 3954986..3955201 (-) 216 WP_001008320.1 DUF3912 family protein -
  L6481_RS20360 (BCM0060_4022) - 3955454..3955765 (+) 312 WP_001093243.1 hypothetical protein -
  L6481_RS20365 (BCM0060_4023) - 3955802..3956290 (-) 489 WP_000764279.1 hypothetical protein -
  L6481_RS20370 (BCM0060_4024) - 3956370..3956744 (-) 375 WP_001012350.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39412.97 Da        Isoelectric Point: 9.2147

>NTDB_id=79715 L6481_RS20335 WP_001013200.1 3950955..3951998(-) (comGA) [Bacillus cereus strain MRY14-0060]
MNGIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKYCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=79715 L6481_RS20335 WP_001013200.1 3950955..3951998(-) (comGA) [Bacillus cereus strain MRY14-0060]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTGGTTCAGCTGCGTATAGGAAAAGATTTAATGACGAAATATTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCTATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTGCGTCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
GTTATTCTCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAATGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366

Multiple sequence alignment