Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   L6481_RS19150 Genome accession   NZ_AP022877
Coordinates   3751589..3752023 (-) Length   144 a.a.
NCBI ID   WP_061531485.1    Uniprot ID   -
Organism   Bacillus cereus strain MRY14-0060     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3746589..3757023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6481_RS19135 (BCM0060_3781) - 3747353..3747910 (+) 558 WP_000062080.1 PadR family transcriptional regulator -
  L6481_RS19140 (BCM0060_3782) metE 3748838..3751126 (+) 2289 WP_001007632.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  L6481_RS19145 (BCM0060_3783) comJ 3751163..3751552 (-) 390 WP_001995060.1 competence protein ComJ -
  L6481_RS19150 (BCM0060_3784) nucA/comI 3751589..3752023 (-) 435 WP_061531485.1 DNA-entry nuclease Machinery gene
  L6481_RS19155 (BCM0060_3785) - 3752260..3753009 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  L6481_RS19160 (BCM0060_3786) kinB 3753063..3754337 (-) 1275 WP_236806600.1 sporulation sensor histidine kinase KinB -
  L6481_RS19165 (BCM0060_3787) - 3754648..3754848 (+) 201 WP_000929284.1 hypothetical protein -
  L6481_RS19170 (BCM0060_3788) - 3754971..3756134 (+) 1164 WP_000434590.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16040.40 Da        Isoelectric Point: 8.4331

>NTDB_id=79712 L6481_RS19150 WP_061531485.1 3751589..3752023(-) (nucA/comI) [Bacillus cereus strain MRY14-0060]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAVDRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=79712 L6481_RS19150 WP_061531485.1 3751589..3752023(-) (nucA/comI) [Bacillus cereus strain MRY14-0060]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGTGGATAGAAGAAAG
TTATCGTTAGCTCCGTACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(50-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment