Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   L6481_RS06035 Genome accession   NZ_AP022877
Coordinates   1195427..1197253 (+) Length   608 a.a.
NCBI ID   WP_000003393.1    Uniprot ID   A0A0G8DIA9
Organism   Bacillus cereus strain MRY14-0060     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1190427..1202253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6481_RS06015 (BCM0060_1098) - 1190749..1191423 (-) 675 WP_000362618.1 TerC family protein -
  L6481_RS06020 (BCM0060_1099) mecA 1191750..1192433 (+) 684 WP_000350710.1 adaptor protein MecA Regulator
  L6481_RS06025 (BCM0060_1100) - 1192506..1194050 (+) 1545 WP_000799201.1 cardiolipin synthase -
  L6481_RS06030 (BCM0060_1101) - 1194132..1195376 (+) 1245 WP_042513094.1 competence protein CoiA family protein -
  L6481_RS06035 (BCM0060_1102) pepF 1195427..1197253 (+) 1827 WP_000003393.1 oligoendopeptidase F Regulator
  L6481_RS06040 (BCM0060_1103) - 1197374..1197547 (-) 174 WP_002051510.1 hypothetical protein -
  L6481_RS06045 (BCM0060_1104) - 1197777..1198670 (-) 894 WP_000360638.1 ClpXP adapter SpxH family protein -
  L6481_RS06050 (BCM0060_1105) - 1198670..1199068 (-) 399 WP_000043381.1 hypothetical protein -
  L6481_RS06055 (BCM0060_1106) - 1199249..1199827 (-) 579 WP_000191105.1 CYTH domain-containing protein -
  L6481_RS06060 (BCM0060_1107) - 1199974..1200345 (+) 372 WP_001180009.1 hypothetical protein -
  L6481_RS06065 (BCM0060_1108) - 1200376..1201014 (+) 639 WP_001081482.1 GTP pyrophosphokinase family protein -
  L6481_RS06070 (BCM0060_1109) - 1201033..1201830 (+) 798 WP_000673194.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70334.62 Da        Isoelectric Point: 4.6458

>NTDB_id=79699 L6481_RS06035 WP_000003393.1 1195427..1197253(+) (pepF) [Bacillus cereus strain MRY14-0060]
MSEQNTAKTLPDRNEIEESSTWRLEDIFQTDTEWEKEFQAIKELLPKLTEFKGKLGDSANNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSASSNTFGMLNNADLKFPSIKGEDGEEVEITHGRYIQFLESDDRRVREDAFKAVYETYGKYKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLIESVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDMLLKSLHVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=79699 L6481_RS06035 WP_000003393.1 1195427..1197253(+) (pepF) [Bacillus cereus strain MRY14-0060]
ATGTCTGAACAAAATACAGCAAAAACATTACCAGATCGTAACGAGATTGAAGAATCAAGTACGTGGAGATTGGAAGATAT
TTTCCAAACGGATACAGAGTGGGAAAAAGAATTCCAAGCTATTAAAGAGTTATTACCGAAGTTAACTGAATTTAAAGGGA
AACTTGGTGACTCTGCGAACAATTTACTTGAAGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGTTATAT
ACATATGCACATATGCGTTACGATCAAGATACAACAAACTCTGTGTATCAAGCGTTAAATGACCGCGCGACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCATATATTGTGCCTGAAATTTTATCGATTTCAGAAGATACACTGCAAACATTCTTAA
AAGAAAATAGAGACTTAAGTGTATATGAACATGCATTAGAAGAAATTACGCGCCAACGTCCGCACGTATTATCTGAAGCG
GAAGAGGCTTTATTAGCAGAAGCATCTGAAGTAATGAGTGCGTCAAGTAATACATTTGGTATGTTGAATAATGCGGATTT
GAAATTCCCATCTATTAAAGGTGAGGATGGAGAAGAAGTAGAGATTACACATGGTCGTTACATTCAGTTTTTAGAAAGTG
ATGATCGTCGTGTGCGCGAAGATGCATTTAAAGCTGTATATGAAACGTACGGAAAATATAAGAATACATTTGCAAGTACG
TTAAGCGGAGCTGTGAAACGTAATAATTTTAATGCACGCGTTCGTAAATACGACTCTGCACGTCAAGCGGCATTAAGTAA
TAATAATATTCCTGAAGCAGTGTACGATCAACTTATTGAATCTGTAAATGATAATTTACACTTGTTACATCGTTACATTG
ATATTCGTAAGCGTGCACTAGGGCTTGATGAGCTTCATATGTATGATTTATATACACCGCTTGTACCAGAAGTGAAAATG
AACGTGAAGTATGAAGAAGCGCAAGACATGTTATTAAAATCTTTACATGTACTTGGTGATGAGTATGTTGATATTTTAAA
AGAAGCATATGAAAATCGTTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGAGCATATTCATCTGGTGCATATG
GAACAAATCCTTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAATTTGGTCATTCT
GTGCATAGTTACTATACAAGAAAAACGCAGCCGCACGTATACGGTGACTATTCAATATTTGTTGCGGAAGTAGCATCAAC
CTGTAATGAGGCGTTGCTAAACGATTACTTATTAAAAACGACAGAAGATAAGAAAGAACGTCTATACTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACGATGTTCGCAGAGTTTGAGCATATTATTCATAAGAAAGTACAA
GAGGGACATGCGGTTACGCCAGACATGTTAACGGAGATCTACTATGATTTAAATAAGAAATATTTCGGTGATGCTTTAGT
AATTGATGAAGAAATTGGTTTAGAGTGGTCACGTATTCCGCACTTCTACTACAACTATTACGTATACCAATACGCAACAG
GATTTAGTGCAGCAACTGCTCTATCTAAACAAATTTTAGAAGAAGGGCAACCAGCAGTAGAACGCTATATTAATGAGTTC
TTAAAGGCAGGAAGTTCGGATTATCCAATTGAAGTGCTGAAAAAAGCTGGAGTAGATATGGCATCTCCAGAACCGGTAAA
AGAAGCATTGCAAGTGTTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAGAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8DIA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.084

97.533

0.488

Multiple sequence alignment