Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PYW33_RS12905 Genome accession   NZ_CP118963
Coordinates   2671570..2672370 (-) Length   266 a.a.
NCBI ID   WP_004647462.1    Uniprot ID   -
Organism   Acinetobacter lwoffii strain DSM 2403     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2666570..2677370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW33_RS12885 - 2667615..2668313 (-) 699 WP_004647466.1 YfgM family protein -
  PYW33_RS12890 hisS 2668344..2669639 (-) 1296 WP_004647465.1 histidine--tRNA ligase -
  PYW33_RS12895 ispG 2669643..2670764 (-) 1122 WP_004647464.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  PYW33_RS12900 - 2670788..2671579 (-) 792 WP_004647463.1 helix-turn-helix domain-containing protein -
  PYW33_RS12905 pilF 2671570..2672370 (-) 801 WP_004647462.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  PYW33_RS12910 rlmN 2672385..2673620 (-) 1236 WP_004647461.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  PYW33_RS12915 ndk 2673743..2674174 (-) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  PYW33_RS12920 iscX 2674285..2674485 (-) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  PYW33_RS12925 - 2674573..2675124 (-) 552 WP_004278361.1 phosphatase PAP2 family protein -
  PYW33_RS12930 - 2675145..2676425 (-) 1281 WP_004647459.1 glycosyltransferase family 4 protein -
  PYW33_RS12935 - 2676694..2677110 (-) 417 WP_004647457.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30163.29 Da        Isoelectric Point: 9.2878

>NTDB_id=796929 PYW33_RS12905 WP_004647462.1 2671570..2672370(-) (pilF) [Acinetobacter lwoffii strain DSM 2403]
MRNLTSKFTLISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALESSPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDT
ANAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWIGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=796929 PYW33_RS12905 WP_004647462.1 2671570..2672370(-) (pilF) [Acinetobacter lwoffii strain DSM 2403]
TTGAGAAATCTAACATCAAAGTTTACTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATCTGAGAACAGGTGACCTGG
ATGCAGCTAAACGTGCGCTGGATCAGGCACTTGAAAGCAGTCCGCGTGATTCTCAAGCGAATATGATGATGGGCGTGCTA
CTGCAACAGGAAGGTAGTAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCGCAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCAATCGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAGCGATTCCGCGCACTGGAAAATATGGGGCGGATTTACCTGAAACTGGGTGATACA
GCAAATGCTGAAAAATCATTCAAACAGGCGCTACAAGCCAATCGCGATTCCTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCAGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGGCAGAAAAATCAGG
GTGCCCGTGCACTCTGGATTGGCATTCGCACTGCGCGTGCCGGAGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTCTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(42-81)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.846

97.744

0.771
  pilF Acinetobacter baylyi ADP1

70.301

100

0.703