Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PYW32_RS10430 Genome accession   NZ_CP118957
Coordinates   2032286..2034103 (-) Length   605 a.a.
NCBI ID   WP_016174352.1    Uniprot ID   -
Organism   Enterococcus saccharolyticus subsp. saccharolyticus strain DSM 20726     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2027286..2039103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW32_RS10410 - 2027681..2028166 (+) 486 WP_016174356.1 GNAT family N-acetyltransferase -
  PYW32_RS10415 - 2028241..2029599 (-) 1359 WP_016174355.1 TrkH family potassium uptake protein -
  PYW32_RS10420 - 2029781..2030926 (+) 1146 WP_016174354.1 cation:proton antiporter -
  PYW32_RS10425 - 2031094..2032242 (+) 1149 WP_016174353.1 cation:proton antiporter -
  PYW32_RS10430 pepF 2032286..2034103 (-) 1818 WP_016174352.1 oligoendopeptidase F Regulator
  PYW32_RS10435 - 2034166..2035137 (-) 972 WP_016174351.1 competence protein CoiA -
  PYW32_RS10440 - 2035259..2035972 (-) 714 WP_016174350.1 adaptor protein MecA -
  PYW32_RS10445 spxA 2036126..2036524 (-) 399 WP_016174349.1 transcriptional regulator SpxA -
  PYW32_RS10450 - 2037136..2038851 (-) 1716 WP_016174348.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 605 a.a.        Molecular weight: 68881.05 Da        Isoelectric Point: 4.5625

>NTDB_id=796808 PYW32_RS10430 WP_016174352.1 2032286..2034103(-) (pepF) [Enterococcus saccharolyticus subsp. saccharolyticus strain DSM 20726]
MSETKQLPTREEVAEASTWDLTKIFVDDAAFEVAFKELQAELKQASSYKGTLNKGGAAFLAALEYVLDVSRKLETLYVYS
HLKNDQDTANTTYQALYARASSLLAQTSEAISWLEPELLTLSDETIWSYFDEEPKLEGYRHFVKQTVDNRAHVLPAEQEA
LLAAAGEIFGSPSNTFSVLNNADLVFPTIEGEDGEKIQLSHGIYGQLLESTDRRVREEAFKGLYSVYEQFRNTFASTLSA
NVKAHNYKAKVRKFASAREAALSSNHIPESVYDTLVDVVNQNLPLLHRYMELRKRLLNLDDLHMYDVYTPLLGEAPISYT
YDQAVEKAIQALEPMGEEYLTVVKEAFNNRWIDVVENKGKRSGAYSSGAYDTLPYILMNWHDTLDQLFTLVHEMGHSVHS
YFTRSNQPYVYGDYSIFLAEIASTTNENILTEHLLETETDPRVRAYVLNHYLDGFKGTVFRQTQFAEFEHFMHVEDAKGT
PLTSEFLSESYGALNKKYYGEALADDPEIRLEWSRIPHFYYNYYVFQYSTGFSAASALAGKILSEGPAALERYLNYLKAG
NSDYPIEVMKKAGVDMTQADYIEDAMKMFEQRLNELEALVEELEN

Nucleotide


Download         Length: 1818 bp        

>NTDB_id=796808 PYW32_RS10430 WP_016174352.1 2032286..2034103(-) (pepF) [Enterococcus saccharolyticus subsp. saccharolyticus strain DSM 20726]
ATGTCAGAAACAAAACAATTACCAACACGTGAAGAAGTCGCAGAAGCGTCAACATGGGATTTAACGAAAATTTTTGTGGA
TGATGCAGCTTTCGAAGTTGCATTTAAAGAATTACAAGCAGAATTAAAACAAGCATCATCTTACAAAGGAACCTTGAACA
AGGGTGGTGCTGCCTTTTTAGCAGCATTAGAATATGTCTTAGATGTCTCACGTAAATTAGAAACATTGTATGTTTACAGT
CATTTAAAAAATGATCAAGATACGGCAAATACAACATACCAAGCGCTTTATGCTCGTGCAAGTAGTTTATTAGCACAAAC
GAGTGAAGCTATCTCTTGGTTAGAACCAGAATTGTTGACACTAAGTGATGAAACAATTTGGAGCTATTTTGACGAAGAAC
CAAAATTAGAAGGGTATCGTCATTTTGTGAAACAAACGGTAGACAATCGTGCACATGTGTTACCGGCGGAACAAGAAGCA
TTGCTAGCTGCAGCAGGAGAAATTTTTGGCTCACCAAGCAATACTTTCTCAGTCTTAAATAATGCCGATTTAGTTTTCCC
AACCATTGAAGGCGAAGACGGAGAAAAAATTCAATTATCACATGGTATTTATGGTCAATTATTAGAAAGTACTGACCGTC
GTGTACGTGAAGAAGCATTTAAAGGTTTATACAGTGTTTACGAGCAATTCCGGAACACCTTTGCTTCTACTTTAAGTGCG
AATGTGAAAGCACACAATTACAAAGCAAAAGTACGCAAATTTGCTTCTGCACGTGAAGCGGCATTAAGTAGTAACCATAT
TCCTGAAAGTGTTTATGATACATTAGTGGATGTAGTGAATCAAAATCTACCATTGTTACATCGTTACATGGAACTGCGCA
AACGTTTGTTAAATCTCGATGATTTGCACATGTACGATGTATATACGCCTTTATTAGGTGAAGCACCAATTAGTTATACG
TATGACCAAGCGGTAGAAAAAGCGATTCAAGCGCTAGAGCCAATGGGTGAAGAGTATCTAACTGTTGTGAAAGAAGCCTT
TAATAATCGTTGGATTGATGTGGTTGAGAATAAAGGAAAACGTAGTGGTGCGTATTCTTCAGGAGCATATGATACCTTGC
CATATATTTTAATGAATTGGCATGATACTTTGGATCAATTATTTACGTTGGTTCATGAAATGGGACATAGTGTTCATAGT
TACTTTACTCGTAGCAATCAACCTTATGTGTATGGAGATTATTCGATTTTCTTAGCAGAAATTGCTTCAACTACCAATGA
AAATATTTTAACTGAACATTTGTTAGAAACTGAAACTGATCCACGTGTTCGCGCATATGTGTTAAATCATTATTTAGATG
GTTTTAAAGGAACAGTCTTCCGTCAAACACAATTTGCGGAATTTGAACATTTTATGCATGTTGAAGATGCAAAAGGTACA
CCATTAACAAGCGAATTTTTAAGTGAAAGTTATGGCGCTTTAAATAAAAAATATTATGGCGAAGCACTAGCTGATGATCC
GGAAATTCGTTTAGAATGGTCACGTATTCCTCATTTCTATTACAATTATTATGTTTTCCAATATTCAACAGGATTTTCTG
CTGCATCTGCTTTAGCCGGAAAAATTCTTTCAGAAGGACCAGCTGCATTAGAGCGTTACTTAAACTATCTAAAAGCTGGA
AATAGCGATTATCCAATCGAAGTCATGAAAAAAGCCGGTGTTGATATGACACAAGCAGACTATATTGAAGACGCGATGAA
GATGTTTGAACAACGTTTGAATGAATTAGAAGCATTAGTCGAAGAATTAGAGAACTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(207-587)

Oligopeptidase F (Peptidase_M3_N)

(117-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

55.649

98.017

0.545