Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PYW32_RS00150 Genome accession   NZ_CP118957
Coordinates   30393..31763 (+) Length   456 a.a.
NCBI ID   WP_016176063.1    Uniprot ID   S0JGW6
Organism   Enterococcus saccharolyticus subsp. saccharolyticus strain DSM 20726     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 25393..36763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW32_RS00125 - 26084..26284 (-) 201 WP_245558504.1 hypothetical protein -
  PYW32_RS00130 - 26360..27604 (-) 1245 WP_016176059.1 glutamate-5-semialdehyde dehydrogenase -
  PYW32_RS00135 proB 27607..28407 (-) 801 WP_016176060.1 glutamate 5-kinase -
  PYW32_RS00140 - 28657..29637 (+) 981 WP_016176061.1 P1 family peptidase -
  PYW32_RS00145 - 29878..30381 (+) 504 WP_016176062.1 dUTP pyrophosphatase -
  PYW32_RS00150 radA 30393..31763 (+) 1371 WP_016176063.1 DNA repair protein RadA Machinery gene
  PYW32_RS00155 - 31843..32961 (+) 1119 WP_016176064.1 PIN/TRAM domain-containing protein -
  PYW32_RS00160 istB 33409..34188 (-) 780 WP_016176065.1 IS21-like element helper ATPase IstB -
  PYW32_RS00165 istA 34200..35420 (-) 1221 WP_016176066.1 IS21 family transposase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49913.56 Da        Isoelectric Point: 7.3022

>NTDB_id=796775 PYW32_RS00150 WP_016176063.1 30393..31763(+) (radA) [Enterococcus saccharolyticus subsp. saccharolyticus strain DSM 20726]
MAKKAKTQFECQHCGYISPRYLGKCPQCGSWNSMVEEKIQDTSDRRSRTTLTGQKMQPMKLADVVPKKEPRVQTQLGELN
RVLGGGVVPGSMVLLGGDPGIGKSTLLLQVSQQLAATGGKVLYVSGEESAEQIKMRAQRLGVVDNEFYLYAETDMHDISR
AIENLEPDYVIIDSIQTMTQPDISSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHQSFRILRAVKNRFGSTNEIGIFEMREGGLEEVANPSQVFLEERLAGATGSAIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTEATECFIGEIGLTG
EIRRVNAIEQRVKEVQKLGFKKVYVPKNNLVNWEPPKGIEVVGLVTLDEALKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=796775 PYW32_RS00150 WP_016176063.1 30393..31763(+) (radA) [Enterococcus saccharolyticus subsp. saccharolyticus strain DSM 20726]
ATGGCAAAAAAAGCAAAAACACAATTTGAATGCCAACACTGTGGGTATATTTCCCCTCGTTATTTAGGAAAATGTCCGCA
ATGTGGCAGTTGGAATTCAATGGTGGAAGAAAAAATTCAAGATACCTCGGATCGTAGGTCACGCACCACTTTGACAGGAC
AAAAGATGCAACCAATGAAATTAGCGGACGTTGTGCCCAAAAAAGAACCCCGTGTCCAAACGCAACTAGGCGAGTTAAAT
CGTGTGTTGGGTGGCGGAGTGGTACCTGGTTCAATGGTATTGCTTGGTGGTGACCCGGGAATTGGGAAATCGACGTTACT
GTTGCAAGTCTCACAACAATTGGCGGCAACCGGCGGCAAAGTCTTATATGTTTCCGGAGAAGAAAGTGCCGAGCAAATTA
AAATGCGGGCGCAACGTTTAGGCGTGGTGGATAATGAATTTTATTTATATGCAGAAACGGACATGCACGATATTAGTCGG
GCGATTGAAAACTTGGAACCCGATTATGTCATTATTGACTCGATTCAAACGATGACACAACCTGATATTAGCAGTGTCGC
AGGAAGTGTGAGTCAAGTTCGTGAAACCACAGCTGAATTGCTGAAAATTGCCAAAACCAATGGCATTGCGATTTTTATTG
TTGGTCATGTGACCAAAGAAGGTTCGATTGCTGGTCCACGAATGTTGGAGCACATGGTTGATACAGTGTTATACTTTGAA
GGAGAACAACATCAAAGTTTCCGAATTTTACGGGCGGTTAAAAATCGTTTTGGTTCAACCAACGAAATTGGTATTTTTGA
AATGCGTGAAGGTGGTTTGGAAGAAGTCGCTAATCCATCACAAGTTTTTCTGGAAGAACGTTTAGCAGGAGCGACGGGTT
CTGCGATTGTCGTAGCAATGGAAGGCAGTCGCCCGATTCTTGTCGAAATCCAAGCTTTAGTCACACCGACAATGTTTGGG
AATGCCAAACGGACAACGACAGGTCTTGATTTCAATCGTGTATCGTTGATTATGGCCGTTTTGGAGAAACGAGCAGGCTT
GTTATTGCAAAATCAAGATGCCTATTTAAAAGCGGCTGGTGGCGTGAAATTAAACGAACCTGCGATTGACTTGGCGATTG
CCGTTAGTATTGCTTCAAGTTACAAAGAAAAAGGCACCGAAGCGACAGAATGTTTTATCGGTGAGATTGGATTAACTGGT
GAAATTCGTCGCGTGAATGCGATTGAACAACGTGTCAAAGAAGTGCAAAAGTTAGGATTTAAAAAAGTCTATGTTCCTAA
AAATAACTTAGTCAATTGGGAACCACCCAAAGGAATTGAAGTTGTTGGTTTAGTAACATTAGATGAAGCATTAAAACGTG
TTTTTCGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S0JGW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

72.527

99.781

0.724
  radA Streptococcus mitis SK321

72.308

99.781

0.721
  radA Streptococcus pneumoniae Rx1

72.308

99.781

0.721
  radA Streptococcus pneumoniae D39

72.308

99.781

0.721
  radA Streptococcus pneumoniae R6

72.308

99.781

0.721
  radA Streptococcus pneumoniae TIGR4

72.308

99.781

0.721
  radA Bacillus subtilis subsp. subtilis str. 168

65.854

98.904

0.651