Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RA165_RS18335 Genome accession   NZ_CP132550
Coordinates   3455832..3456509 (+) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain XL1-Blue     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3450832..3461509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA165_RS18300 gspG 3451312..3451749 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  RA165_RS18305 gspH 3451757..3452266 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  RA165_RS18310 gspI 3452263..3452640 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RA165_RS18315 gspJ 3452633..3453220 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  RA165_RS18320 gspK 3453213..3454196 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  RA165_RS18325 gspL 3454211..3455374 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  RA165_RS18330 gspM 3455371..3455832 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  RA165_RS18335 pilD 3455832..3456509 (+) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  RA165_RS18340 bfr 3456538..3457014 (-) 477 WP_000675504.1 bacterioferritin -
  RA165_RS18345 bfd 3457086..3457280 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RA165_RS18350 chiA 3457449..3460142 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=796727 RA165_RS18335 WP_000178154.1 3455832..3456509(+) (pilD) [Escherichia coli strain XL1-Blue]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=796727 RA165_RS18335 WP_000178154.1 3455832..3456509(+) (pilD) [Escherichia coli strain XL1-Blue]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378