Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PYW30_RS00935 Genome accession   NZ_CP118950
Coordinates   175038..176399 (+) Length   453 a.a.
NCBI ID   WP_074750756.1    Uniprot ID   -
Organism   Lactococcus garvieae subsp. garvieae strain DSM 20684     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 170038..181399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW30_RS00895 - 170307..171566 (+) 1260 WP_042219922.1 IS110 family transposase -
  PYW30_RS00900 infA 171887..172105 (+) 219 WP_003133772.1 translation initiation factor IF-1 -
  PYW30_RS00905 rpmJ 172159..172275 (+) 117 WP_014024193.1 50S ribosomal protein L36 -
  PYW30_RS00910 rpsM 172293..172658 (+) 366 WP_003133773.1 30S ribosomal protein S13 -
  PYW30_RS00915 rpsK 172676..173059 (+) 384 WP_003133774.1 30S ribosomal protein S11 -
  PYW30_RS00920 - 173111..174049 (+) 939 WP_003133775.1 DNA-directed RNA polymerase subunit alpha -
  PYW30_RS00925 rplQ 174065..174445 (+) 381 WP_003133776.1 50S ribosomal protein L17 -
  PYW30_RS00935 radA 175038..176399 (+) 1362 WP_074750756.1 DNA repair protein RadA Machinery gene
  PYW30_RS00940 - 176533..177609 (+) 1077 WP_003133779.1 PIN/TRAM domain-containing protein -
  PYW30_RS00945 - 177632..178261 (+) 630 WP_042217676.1 pyridoxamine 5'-phosphate oxidase family protein -
  PYW30_RS00950 ald 178449..179570 (+) 1122 WP_042217673.1 alanine dehydrogenase -
  PYW30_RS00955 - 179604..180098 (-) 495 WP_014024197.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49767.98 Da        Isoelectric Point: 4.9969

>NTDB_id=796669 PYW30_RS00935 WP_074750756.1 175038..176399(+) (radA) [Lactococcus garvieae subsp. garvieae strain DSM 20684]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGNGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARECFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVDIPENLKVVGVTTLSECLKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=796669 PYW30_RS00935 WP_074750756.1 175038..176399(+) (radA) [Lactococcus garvieae subsp. garvieae strain DSM 20684]
ATAGCTAAAAAAAAATCAACATTTCTTTGCCAAGAATGTGGCTACAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGTGCTTGGGGTTCCTTTGTTGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGAGTGAGTCTGACAGGCGAGCACT
CTAAACCCATGAAGTTAGATCAGGTTGAACTTTTCGATACACCGCGTGTGGAAACCGATCTGGATGAGTTTAACCGCGTC
CTTGGAGGCGGTGTCGTTCCAGGAAGTCTTGTCCTTATTGGGGGGGATCCAGGGATTGGTAAATCTACTCTACTGCTTCA
AGTATCTACGCAACTTGCTTCAAGAGGGCGTGTACTTTATGTCAGTGGGGAGGAGTCGGCTCAACAAATTAAGCTGCGTG
CCGAGCGTTTGGGCGACATCGATACAGATTTTTACCTTTACGCCGAAACCAATATGCAAAGCATCCGAAATGAAGTTGAA
CGTCTACAGCCAGACTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATCCAGAGTACTCAGGGTTCGGT
TAGTCAGGTGCGCGAGGTGACAGGAGAATTAATGCAGTTGGCCAAAACCAACGATATTGCGACCTTTATTGTCGGACATG
TCACTAAGGAAGGGCAACTTGCGGGGCCGCGTATGTTGGAACATATGGTTGATACGGTCTTGTATTTTGAGGGAGAACGT
CAAAATACATTCCGAATTTTACGCGCGGTCAAAAACCGTTTTGGTTCAACAAATGAAATTGGGATTTTCGAGATGCAGGG
CAATGGTTTAGTGGAAGTGACTAACCCGAGTGAAGTTTTCCTCGAAGAGCGTTTGGAAGGCTCTACGGGCTCTGCTATCG
TCTGTGCGTTAGAAGGTACGCGTCCGATATTGGTTGAGATTCAAGCTTTGACAACGCCAACCATGTTTGGAAATGCCAAA
CGTACCACCTCTGGTTTGGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTCGAAAAGCGTGCCGGTTTTCTTTTGCA
ACAACAAGATGCCTACTTAAAATCCGCAGGTGGTGTCAAGTTGGATGAACCAGCCATTGACTTAGCAGTCGCTGTAGCTA
TTGCTTCTAGCTACAAGGAAGAGCCGACAGATGCACGCGAATGTTTTATTGGCGAAATTGGCCTGACGGGTGAAATTCGT
CGTGTTACGCGGATGGAGCAACGTCTCAATGAAGCAAGCAAACTAGGTTTTCAAAAAGTTTACGCGCCTAAGAATAGCTT
GGCAGGCGTAGATATTCCAGAAAATTTGAAAGTGGTCGGTGTGACGACTTTATCAGAATGTTTGAAAAAAGTATTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.353

100

0.804
  radA Streptococcus pneumoniae D39

80.353

100

0.804
  radA Streptococcus pneumoniae R6

80.353

100

0.804
  radA Streptococcus pneumoniae TIGR4

80.353

100

0.804
  radA Streptococcus mitis NCTC 12261

80.353

100

0.804
  radA Streptococcus mitis SK321

80.353

100

0.804
  radA Bacillus subtilis subsp. subtilis str. 168

61.22

100

0.62