Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PUW69_RS01340 Genome accession   NZ_CP118947
Coordinates   269946..271742 (-) Length   598 a.a.
NCBI ID   WP_005725465.1    Uniprot ID   A0A4V3BI87
Organism   Lactobacillus crispatus strain VSI04     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 264946..276742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW69_RS01290 (PUW69_01290) - 264965..265822 (+) 858 WP_005728089.1 IS982-like element ISLh1 family transposase -
  PUW69_RS01295 (PUW69_01295) - 265965..266111 (+) 147 WP_005727423.1 hypothetical protein -
  PUW69_RS01300 (PUW69_01300) - 266121..266267 (+) 147 WP_005723821.1 hypothetical protein -
  PUW69_RS01305 (PUW69_01305) - 266445..267623 (-) 1179 WP_005727388.1 IS256 family transposase -
  PUW69_RS01310 (PUW69_01310) - 267811..268071 (+) 261 WP_005727422.1 thioredoxin domain-containing protein -
  PUW69_RS01315 (PUW69_01315) - 268087..268383 (+) 297 WP_005725464.1 bacteriocin immunity protein -
  PUW69_RS01320 (PUW69_01320) - 268557..268775 (+) 219 WP_005726358.1 TIGR04139 family peptide modification target -
  PUW69_RS01325 (PUW69_01325) - 268779..269054 (+) 276 WP_005723823.1 bacteriocin immunity protein -
  PUW69_RS01330 (PUW69_01330) - 269168..269575 (-) 408 WP_005723824.1 type II toxin-antitoxin system death-on-curing family toxin -
  PUW69_RS01335 (PUW69_01335) - 269579..269794 (-) 216 WP_005723825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  PUW69_RS01340 (PUW69_01340) pepF 269946..271742 (-) 1797 WP_005725465.1 oligoendopeptidase F Regulator
  PUW69_RS01345 (PUW69_01345) - 271908..273143 (+) 1236 WP_005725466.1 LCP family protein -
  PUW69_RS01350 (PUW69_01350) - 273210..273899 (+) 690 WP_005725467.1 DUF554 domain-containing protein -
  PUW69_RS11755 - 273907..274200 (-) 294 WP_162262781.1 PBECR4 domain-containing protein -
  PUW69_RS01355 (PUW69_01355) - 274158..274463 (-) 306 WP_005727415.1 PBECR4 domain-containing protein -
  PUW69_RS01360 (PUW69_01360) - 274778..275326 (-) 549 WP_005727414.1 GNAT family protein -
  PUW69_RS01365 (PUW69_01365) - 275473..275850 (+) 378 WP_005718180.1 hypothetical protein -

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 68019.87 Da        Isoelectric Point: 4.6935

>NTDB_id=796616 PUW69_RS01340 WP_005725465.1 269946..271742(-) (pepF) [Lactobacillus crispatus strain VSI04]
MAIPTRNEVPEELKWDLTRIFKNDDEWEQAYAAAQEKVAKLAELKGTLAKSGKNLYESLTKILAVKRDVENIYVYATMSS
DVDTSNSHYLGYVSRVQSLANQFEAATSFINPEILSIPSDKLEQFKQAEPRLKDYAHYLETITNKRPHTLPAEQEKLIAD
AGDALSVSENTFNVLTNSDMEYGYVQDDDGNMEQLSDGLYSLLIQSQNRDVRKGAFDTLYATYGQFQNSLASTLSGVVKK
HNYNARVHKYNSAREAALADNGVPVAVYDTLIQEVDSHLDLLHRYVALRKKILGLKDLQMWDMYVPLTGRPSLSYNFEEA
KETAKKALAPLGEDYLKHVDYIFNNRVIDPIESKNKVTGAYSGGAYDTDPYELLNWEDNIDSLYTLVHETGHSVHSWYTR
NTQPYVYGDYPIFVAEIASTTNENILTEYFLDHITDPKTRAFILNYYLDSFKGTLFRQTQFAVFEQFLHEADAKGEPLTA
DTLDDVYGQINQHYYGDSVEPGGDIALEWSRIPHFYYNFYVYQYATGFAAATALANKVVHGTPAERDAYLGFLKAGSSDY
PTEIMKRAGVDMTKPDYLEDAFKTFEKRLNEFESLIEK

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=796616 PUW69_RS01340 WP_005725465.1 269946..271742(-) (pepF) [Lactobacillus crispatus strain VSI04]
ATGGCAATTCCAACTAGAAACGAAGTCCCTGAAGAGCTTAAATGGGACTTAACCCGTATTTTTAAAAATGACGATGAATG
GGAGCAAGCTTATGCAGCTGCACAAGAAAAAGTGGCTAAACTTGCTGAATTAAAAGGTACACTAGCTAAATCAGGCAAGA
ACCTGTACGAGAGCTTAACCAAAATCTTAGCTGTTAAACGTGATGTGGAAAATATCTACGTTTACGCTACCATGTCCAGC
GATGTTGATACTTCTAATTCGCACTATCTCGGCTACGTCAGCCGGGTACAAAGTTTGGCTAACCAGTTTGAGGCTGCAAC
AAGTTTTATTAATCCTGAAATCTTGAGTATCCCTAGTGATAAGCTTGAGCAATTTAAGCAAGCTGAACCTAGATTAAAGG
ATTACGCACACTATCTTGAAACAATTACTAACAAGCGTCCTCATACATTACCTGCTGAGCAAGAAAAATTGATCGCCGAT
GCTGGGGATGCTTTAAGTGTTTCTGAAAATACCTTTAACGTTTTGACCAACTCCGACATGGAATACGGTTACGTTCAAGA
CGATGACGGCAATATGGAACAATTATCAGATGGCCTTTATTCTTTGCTGATCCAATCCCAAAATCGTGATGTCAGAAAAG
GTGCTTTTGATACACTTTATGCCACTTATGGACAGTTCCAAAATTCCTTGGCTTCAACTTTGTCTGGCGTTGTAAAAAAG
CACAACTATAACGCACGTGTGCACAAGTATAATTCAGCTCGTGAAGCTGCTTTAGCCGATAATGGCGTACCCGTAGCAGT
TTATGACACTTTAATCCAGGAAGTTGATTCACACCTAGACTTACTTCACCGTTATGTTGCGCTACGTAAAAAGATCTTAG
GCCTGAAGGATCTGCAAATGTGGGACATGTATGTGCCACTAACCGGAAGACCTTCTCTTTCATACAATTTCGAAGAAGCA
AAAGAGACAGCAAAAAAGGCTTTGGCTCCACTTGGTGAAGACTACTTGAAGCATGTTGACTATATTTTTAATAATCGTGT
CATCGACCCAATAGAATCTAAAAACAAGGTAACTGGTGCCTACTCCGGTGGTGCTTATGATACCGATCCTTATGAGTTGC
TCAACTGGGAAGATAACATCGATTCACTTTATACTTTAGTCCACGAAACTGGTCACTCCGTTCACTCCTGGTACACTCGC
AATACTCAACCTTACGTTTACGGCGACTATCCAATCTTTGTGGCTGAAATTGCTTCAACTACTAATGAAAATATTTTGAC
CGAATATTTCTTAGACCACATTACCGATCCTAAGACGCGGGCCTTTATCTTAAATTACTACCTTGATTCATTTAAAGGCA
CTTTATTCCGTCAAACTCAATTTGCCGTGTTTGAACAATTCTTGCACGAAGCCGATGCTAAGGGAGAACCACTGACCGCT
GACACTTTAGACGACGTTTACGGTCAAATTAATCAGCATTATTACGGCGATAGCGTTGAACCAGGCGGCGATATTGCCCT
TGAATGGTCACGAATTCCACACTTCTACTACAATTTCTATGTGTACCAATATGCGACTGGCTTTGCGGCTGCAACGGCAT
TAGCAAACAAGGTGGTTCATGGAACGCCTGCTGAGAGGGATGCCTACCTTGGTTTCCTTAAAGCTGGTTCAAGTGATTAC
CCAACTGAGATCATGAAGCGTGCTGGCGTAGACATGACTAAGCCTGACTACTTAGAAGATGCCTTCAAGACTTTTGAAAA
ACGGTTGAACGAATTTGAAAGCTTGATTGAGAAATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(113-181)

Peptidase family M3 (Peptidase_M3)

(203-583)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V3BI87

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.602

99.164

0.512