Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PY772_RS21180 Genome accession   NZ_CP118939
Coordinates   4499419..4500858 (-) Length   479 a.a.
NCBI ID   WP_041211850.1    Uniprot ID   A0A2L0TRY9
Organism   Aeromonas caviae strain K524     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4494419..4505858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY772_RS21150 (PY772_21150) - 4494698..4495438 (+) 741 WP_223916237.1 KDGP aldolase family protein -
  PY772_RS21155 (PY772_21155) - 4495542..4497446 (+) 1905 WP_104452180.1 HTH domain-containing protein -
  PY772_RS21160 (PY772_21160) npr 4497462..4497734 (-) 273 WP_010675868.1 PTS phosphocarrier protein NPr -
  PY772_RS21165 (PY772_21165) rapZ 4497755..4498621 (-) 867 WP_104452179.1 RNase adapter RapZ -
  PY772_RS21170 (PY772_21170) ptsN 4498662..4499108 (-) 447 WP_223916235.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PY772_RS21175 (PY772_21175) hpf 4499111..4499398 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  PY772_RS21180 (PY772_21180) rpoN/rpoN1 4499419..4500858 (-) 1440 WP_041211850.1 RNA polymerase factor sigma-54 Machinery gene
  PY772_RS21185 (PY772_21185) lptB 4500924..4501649 (-) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  PY772_RS21190 (PY772_21190) lptA 4501662..4502180 (-) 519 WP_010675873.1 lipopolysaccharide transport periplasmic protein LptA -
  PY772_RS21195 (PY772_21195) lptC 4502167..4502727 (-) 561 WP_039040473.1 LPS export ABC transporter periplasmic protein LptC -
  PY772_RS21200 (PY772_21200) kdsC 4502724..4503278 (-) 555 WP_042015859.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PY772_RS21205 (PY772_21205) - 4503278..4504273 (-) 996 WP_029314845.1 KpsF/GutQ family sugar-phosphate isomerase -
  PY772_RS21210 (PY772_21210) mlaF 4504480..4505280 (+) 801 WP_029314846.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53731.54 Da        Isoelectric Point: 4.3728

>NTDB_id=796576 PY772_RS21180 WP_041211850.1 4499419..4500858(-) (rpoN/rpoN1) [Aeromonas caviae strain K524]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSQAETQDASQLDSSDAMAQESMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTATLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLDEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=796576 PY772_RS21180 WP_041211850.1 4499419..4500858(-) (rpoN/rpoN1) [Aeromonas caviae strain K524]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGAGG
CGCAGGAAACCAGTCAGGCAGAGACACAGGACGCCAGCCAACTCGACTCCAGCGATGCCATGGCCCAGGAGAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGACGAGGTCTACTCGGCCGGTACAGCTCAGAGTGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAGACCACCGAGAACCTGCAAGACTACCTGCTGTGGCAGATGCGGCTCACCCCGTTCA
GTGATGTGGATGCTGCCATCGCCCTTGCCATCATCGATGCCATCGACGAATCCGGGTATCTGACTGCCACCCTCGAGGAT
ATTCAGGCTGCAGTCAGCAGCGAGGAGGTAGAAGTCGAACTGGATGAGATCGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGTATCGCCGCCCGTTCGGTGCAAGAGTGTCTGCTGATCCAGCTCGGCCAGTATTCGCCGGACCTGCCCT
GGCTCGACGAAGCCAGAGAGGTGATCCGCGAACACATGGACCTGCTCGGCAATCGCGACTACCGCACCCTGGCTCGCAAG
ACCCGCCTCAAGGAGAGCGATCTCAAGGAGGTGCTGGATCTCATCCAGCAACTGGAGCCCAGACCCGGCAACGGCATCAT
CCAGAGTGAATCCCAGTACGTGATCCCGGATGTGTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTTGAACTCAATCCGG
ATTCTGCTCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCGCGCAATGCCCGCAGCAGCACGGATACCCAGTTC
ATCCGTTCTCATTTGCAGGAGGCCAAGTGGTTCATCAAGAGCCTGGAGAGTCGTAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAGTACGGCGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCGG
AAGCTGTGGAGATGCATGAGTCGACCATTTCACGGGTCACGACCCAGAAGTACATGCATACGCCCCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAGGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAAAACCCCGCCAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TRY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.472

100

0.482