Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PX335_RS02585 Genome accession   NZ_CP118933
Coordinates   542023..542823 (+) Length   266 a.a.
NCBI ID   WP_199977444.1    Uniprot ID   -
Organism   Acinetobacter pittii strain ML4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 537023..547823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PX335_RS02560 (PX335_02560) - 537939..539228 (+) 1290 WP_075383343.1 glycosyltransferase family 1 protein -
  PX335_RS02565 (PX335_02565) - 539253..539804 (+) 552 WP_005066176.1 phosphatase PAP2 family protein -
  PX335_RS02570 (PX335_02570) iscX 539904..540104 (+) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  PX335_RS02575 (PX335_02575) ndk 540218..540649 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  PX335_RS02580 (PX335_02580) rlmN 540773..542008 (+) 1236 WP_017385733.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  PX335_RS02585 (PX335_02585) pilF 542023..542823 (+) 801 WP_199977444.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  PX335_RS02590 (PX335_02590) - 542814..543605 (+) 792 WP_017385735.1 RodZ domain-containing protein -
  PX335_RS02595 (PX335_02595) ispG 543627..544742 (+) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  PX335_RS02600 (PX335_02600) hisS 544739..546031 (+) 1293 WP_017385736.1 histidine--tRNA ligase -
  PX335_RS02605 (PX335_02605) - 546060..546758 (+) 699 WP_017385737.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30086.32 Da        Isoelectric Point: 9.0417

>NTDB_id=796497 PX335_RS02585 WP_199977444.1 542023..542823(+) (pilF) [Acinetobacter pittii strain ML4]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=796497 PX335_RS02585 WP_199977444.1 542023..542823(+) (pilF) [Acinetobacter pittii strain ML4]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTCAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

94.361

100

0.944
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744