Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JTV28_RS12075 Genome accession   NZ_AP022873
Coordinates   2343325..2344239 (+) Length   304 a.a.
NCBI ID   WP_203472577.1    Uniprot ID   A0A7G1H5Q5
Organism   Dissulfurispira thermophila strain T55J     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2338325..2349239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JTV28_RS12055 (JZK55_23700) mreC 2338638..2339414 (-) 777 WP_203472574.1 rod shape-determining protein MreC -
  JTV28_RS12060 (JZK55_23710) - 2339414..2340445 (-) 1032 WP_277950191.1 rod shape-determining protein -
  JTV28_RS12065 (JZK55_23720) - 2340455..2340886 (-) 432 WP_203472575.1 RDD family protein -
  JTV28_RS12070 (JZK55_23730) - 2341451..2343190 (+) 1740 WP_203472576.1 hypothetical protein -
  JTV28_RS12075 (JZK55_23740) pilA 2343325..2344239 (+) 915 WP_203472577.1 signal recognition particle-docking protein FtsY Machinery gene
  JTV28_RS12085 (JZK55_23750) - 2344406..2345620 (-) 1215 WP_203472578.1 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II -
  JTV28_RS12090 (JZK55_23760) - 2345691..2345873 (-) 183 WP_203472579.1 hypothetical protein -
  JTV28_RS12095 (JZK55_23770) - 2345958..2346818 (+) 861 WP_203472580.1 GAF domain-containing protein -
  JTV28_RS12100 (JZK55_23780) - 2346818..2348551 (+) 1734 WP_207105961.1 GspE/PulE family protein -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 32758.89 Da        Isoelectric Point: 5.0061

>NTDB_id=79632 JTV28_RS12075 WP_203472577.1 2343325..2344239(+) (pilA) [Dissulfurispira thermophila strain T55J]
MGFLDKLKSSLTKTKKNIVERVESILLGRSIDESVIDDIEEVLITSDVGTEATNEIITLLREKIKVGEIKNSEDVRSYLK
TEMALLLGKPQPLIPFGEKPFVILAVGVNGVGKTTTIGKLASRFISEGNSVILAAADTFRAAAIEQLEIWAKRSGADIIK
HQSGSDPAAVAFDAVEAAKARGVDIVIIDTAGRLHTKSQLMDELKKINRVIKKSIPDAPQEILLIVDATTGQNALRQAQI
FNEAIGITGIALTKLDGTAKGGIIFAIKKELGIPVRLIGVGEGVDDLKDFDPEDFVAALFDYNQ

Nucleotide


Download         Length: 915 bp        

>NTDB_id=79632 JTV28_RS12075 WP_203472577.1 2343325..2344239(+) (pilA) [Dissulfurispira thermophila strain T55J]
ATGGGATTCTTAGACAAACTAAAATCAAGTCTTACAAAGACAAAAAAGAATATTGTTGAAAGGGTTGAGTCAATACTTCT
TGGCAGAAGTATTGACGAATCAGTTATTGATGACATTGAAGAAGTTCTCATAACATCAGATGTTGGAACCGAGGCAACAA
ACGAAATAATCACTCTTCTCAGAGAAAAAATAAAAGTAGGCGAGATAAAGAATTCGGAAGATGTAAGGTCTTATTTGAAG
ACTGAGATGGCTTTATTGCTTGGTAAACCACAGCCTCTTATCCCATTTGGAGAAAAGCCTTTTGTGATTTTAGCTGTTGG
CGTTAATGGTGTAGGCAAGACCACAACTATTGGCAAGCTTGCGTCAAGATTCATATCAGAAGGAAACTCTGTAATCCTTG
CTGCAGCAGATACATTCAGGGCTGCTGCAATTGAGCAACTCGAGATATGGGCAAAGCGATCAGGAGCAGATATAATAAAG
CATCAGAGCGGCTCTGACCCTGCTGCTGTTGCCTTTGATGCAGTAGAGGCTGCGAAAGCAAGGGGAGTCGATATAGTGAT
TATTGATACAGCGGGAAGACTCCATACAAAGAGCCAATTAATGGATGAACTTAAAAAGATCAACCGTGTTATAAAAAAAT
CCATACCTGATGCACCACAAGAGATTTTACTTATAGTAGATGCCACCACAGGGCAGAACGCATTAAGACAGGCACAAATA
TTCAACGAGGCAATAGGAATAACGGGCATTGCCCTTACAAAACTCGACGGCACTGCAAAAGGTGGGATTATCTTTGCAAT
AAAAAAGGAGCTTGGCATACCTGTGAGGCTTATAGGTGTTGGTGAAGGAGTTGATGATCTAAAGGACTTTGACCCAGAGG
ACTTTGTTGCAGCACTGTTTGACTATAATCAGTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(100-300)

SRP54-type protein, helical bundle domain (SRP54_N)

(21-83)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G1H5Q5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.355

100

0.484

Multiple sequence alignment