Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PYH58_RS14065 Genome accession   NZ_CP118813
Coordinates   2796069..2797439 (-) Length   456 a.a.
NCBI ID   WP_025904845.1    Uniprot ID   A0A2T4RU06
Organism   Mammaliicoccus sciuri strain 7061     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2791069..2802439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYH58_RS14040 (PYH58_14045) cysE 2791462..2792103 (-) 642 WP_353462960.1 serine O-acetyltransferase -
  PYH58_RS14045 (PYH58_14050) gltX 2792358..2793812 (-) 1455 WP_353462961.1 glutamate--tRNA ligase -
  PYH58_RS14050 (PYH58_14055) ispF 2793828..2794307 (-) 480 WP_103360793.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PYH58_RS14055 (PYH58_14060) ispD 2794300..2794986 (-) 687 WP_262648867.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  PYH58_RS14060 (PYH58_14065) - 2794988..2796046 (-) 1059 WP_037589912.1 PIN/TRAM domain-containing protein -
  PYH58_RS14065 (PYH58_14070) radA 2796069..2797439 (-) 1371 WP_025904845.1 DNA repair protein RadA Machinery gene
  PYH58_RS14070 (PYH58_14075) - 2797595..2800048 (-) 2454 WP_353462962.1 ATP-dependent Clp protease ATP-binding subunit -
  PYH58_RS14075 (PYH58_14080) - 2800065..2801072 (-) 1008 WP_353462963.1 protein arginine kinase -
  PYH58_RS14080 (PYH58_14085) - 2801073..2801609 (-) 537 WP_353462964.1 excinuclease ABC subunit B -
  PYH58_RS14085 (PYH58_14090) - 2801624..2802085 (-) 462 WP_025904841.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50477.24 Da        Isoelectric Point: 7.7103

>NTDB_id=795510 PYH58_RS14065 WP_025904845.1 2796069..2797439(-) (radA) [Mammaliicoccus sciuri strain 7061]
MAKKKVIFECLACGYQSPKWMGKCPNCGAWNQMEESIEHKETKGPKNTFKSDVPAQKIEKLKDVKEEDTPRIKTKINEFN
RVLGGGVVEGSLVLIGGDPGIGKSTLLLQICAALSQQKNVLYVSGEESVRQTKLRAERLVEDSGELNVYAETNLEIIHSM
VQKEKPDVLVIDSIQTVFHPEVTSAPGSVSQVRECTQNLMHIAKQMNIATFIVGHVTKDGQIAGPRLLEHMVDTVLYFEG
DTHHAYRILRAVKNRFGSTNEMGIFEMKQTGLNEVKNPSEMFLEERTKHVSGSTIVATMEGTRPILVEVQALVTPTTFHN
PRRMATGIDHNRLSLLMAVLEKKENFLLQQQDAYIKIAGGVKLSEPAVDLSIVIAIASSFQDKPTKGDDCFVGEVGLTGE
VRRVARIDQRVQEAAKLGFKRIIIPKNNIKGLEFPEGTEVIGVSTTREALKIAFNA

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=795510 PYH58_RS14065 WP_025904845.1 2796069..2797439(-) (radA) [Mammaliicoccus sciuri strain 7061]
ATGGCCAAAAAGAAAGTAATATTTGAGTGTTTAGCCTGCGGATATCAATCTCCAAAATGGATGGGGAAATGCCCTAATTG
TGGTGCATGGAACCAAATGGAAGAATCAATAGAACATAAAGAAACTAAAGGTCCTAAAAATACATTCAAGTCAGATGTAC
CCGCACAAAAAATAGAAAAATTAAAAGACGTTAAAGAAGAAGATACACCTAGAATTAAGACTAAAATCAATGAATTTAAT
CGCGTATTAGGTGGCGGTGTTGTAGAAGGTTCGCTTGTACTCATTGGTGGGGACCCTGGTATTGGTAAATCCACATTATT
ATTACAAATTTGTGCGGCTTTATCTCAACAAAAAAATGTCTTATATGTTTCAGGTGAAGAATCTGTAAGACAAACAAAAT
TAAGAGCTGAAAGATTAGTTGAAGATTCAGGAGAATTAAATGTTTATGCTGAAACGAATTTAGAGATTATACATAGTATG
GTTCAAAAAGAAAAACCAGATGTATTAGTAATAGATTCCATTCAAACTGTCTTCCACCCTGAAGTCACTTCTGCGCCAGG
TTCTGTTTCTCAAGTTAGAGAATGTACACAAAATTTAATGCATATCGCAAAACAAATGAATATTGCTACATTTATTGTTG
GACACGTAACGAAAGATGGACAAATTGCTGGTCCACGTTTGTTAGAACATATGGTAGATACAGTATTATATTTTGAAGGC
GATACACATCATGCATACAGAATATTAAGAGCAGTCAAAAACCGTTTTGGTTCGACAAATGAAATGGGTATTTTTGAAAT
GAAGCAAACGGGTCTTAACGAAGTTAAAAATCCGTCTGAAATGTTCTTAGAAGAAAGAACGAAACATGTATCGGGTTCGA
CTATTGTGGCAACTATGGAAGGTACGAGACCTATTTTAGTTGAAGTACAAGCTTTAGTGACGCCAACGACTTTTCATAAT
CCACGAAGAATGGCAACAGGTATCGATCACAATAGATTAAGCTTATTGATGGCTGTATTAGAGAAGAAAGAGAATTTCTT
ATTACAACAACAAGACGCATATATCAAAATTGCCGGTGGTGTAAAATTAAGTGAACCCGCAGTAGATTTAAGTATTGTTA
TCGCGATAGCTTCAAGTTTCCAAGATAAACCTACTAAAGGTGATGATTGTTTCGTAGGAGAGGTTGGCTTAACAGGTGAA
GTAAGAAGGGTTGCCAGAATTGATCAACGTGTACAAGAAGCGGCTAAATTAGGATTTAAACGTATTATCATACCTAAAAA
TAATATTAAAGGTTTAGAATTCCCTGAAGGTACAGAAGTTATTGGCGTATCAACAACAAGAGAAGCATTGAAAATTGCTT
TTAATGCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(349-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T4RU06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.721

100

0.66
  radA Streptococcus pneumoniae Rx1

61.894

99.561

0.616
  radA Streptococcus pneumoniae D39

61.894

99.561

0.616
  radA Streptococcus pneumoniae R6

61.894

99.561

0.616
  radA Streptococcus pneumoniae TIGR4

61.894

99.561

0.616
  radA Streptococcus mitis NCTC 12261

61.894

99.561

0.616
  radA Streptococcus mitis SK321

61.894

99.561

0.616