Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PYH60_RS12340 Genome accession   NZ_CP118800
Coordinates   2453329..2454699 (-) Length   456 a.a.
NCBI ID   WP_282821607.1    Uniprot ID   -
Organism   Mammaliicoccus lentus strain 7066     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2448329..2459699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYH60_RS12315 (PYH60_12315) cysE 2448723..2449364 (-) 642 WP_103268660.1 serine O-acetyltransferase -
  PYH60_RS12320 (PYH60_12320) gltX 2449615..2451072 (-) 1458 WP_147640581.1 glutamate--tRNA ligase -
  PYH60_RS12325 (PYH60_12325) ispF 2451089..2451568 (-) 480 WP_016999406.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PYH60_RS12330 (PYH60_12330) ispD 2451561..2452247 (-) 687 WP_103268658.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  PYH60_RS12335 (PYH60_12335) - 2452249..2453307 (-) 1059 WP_103268657.1 PIN/TRAM domain-containing protein -
  PYH60_RS12340 (PYH60_12340) radA 2453329..2454699 (-) 1371 WP_282821607.1 DNA repair protein RadA Machinery gene
  PYH60_RS12345 (PYH60_12345) - 2454855..2457320 (-) 2466 WP_016999402.1 ATP-dependent Clp protease ATP-binding subunit -
  PYH60_RS12350 (PYH60_12350) - 2457340..2458347 (-) 1008 WP_016999401.1 protein arginine kinase -
  PYH60_RS12355 (PYH60_12355) - 2458349..2458885 (-) 537 WP_016999400.1 UvrB/UvrC motif-containing protein -
  PYH60_RS12360 (PYH60_12360) - 2458900..2459361 (-) 462 WP_016999399.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50475.27 Da        Isoelectric Point: 7.7094

>NTDB_id=795365 PYH60_RS12340 WP_282821607.1 2453329..2454699(-) (radA) [Mammaliicoccus lentus strain 7066]
MAKKKVIFECLACGYQSPKWMGKCPNCGAWNQMEETIEHKDTKGPKNTFKSDVPAQKIEKLKDIKDEDTPRILTKINEFN
RVLGGGVVEGSLVLIGGDPGIGKSTLLLQICAALSQQKNVLYVSGEESVRQTKLRAERLVEDSGELNVYAETNLEIIHSM
VQKEKPDVLVIDSIQTIYHPEVTSAPGSVSQVRECTQNLMHIAKQMNIATFIVGHVTKDGQIAGPRLLEHMVDTVLYFEG
DTHHAYRILRAVKNRFGSTNEMGIFEMKQTGLNEVKNPSEMFLEERTKHVAGSTIVATMEGTRPILVEVQALVTPTTFHN
PRRMATGIDHNRLSLLMAVLEKKENFLLQQQDAYIKIAGGVKLSEPAVDLSIIIAIASSFQDKPTKGDDCFVGEVGLTGE
VRRVARIDQRVQEAAKLGFKRIIVPKNNLKGLEFPEGTQVIGVSTTREALKIAFNA

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=795365 PYH60_RS12340 WP_282821607.1 2453329..2454699(-) (radA) [Mammaliicoccus lentus strain 7066]
ATGGCGAAAAAGAAAGTAATATTTGAATGTTTAGCCTGTGGGTACCAATCGCCAAAATGGATGGGCAAATGTCCTAATTG
TGGCGCATGGAATCAAATGGAAGAAACAATAGAACATAAAGATACAAAAGGGCCTAAAAATACATTTAAATCAGATGTAC
CAGCACAAAAAATAGAAAAATTAAAAGACATTAAAGATGAAGACACACCTAGAATTTTAACGAAAATTAATGAATTTAAC
CGCGTATTAGGCGGCGGAGTAGTAGAAGGGTCGCTTGTATTAATAGGTGGAGATCCGGGTATCGGTAAATCGACACTATT
ATTACAAATTTGTGCCGCTCTATCACAACAGAAAAACGTACTTTACGTCTCTGGTGAGGAATCGGTAAGACAAACTAAAT
TAAGAGCAGAAAGACTCGTTGAAGATTCAGGCGAACTAAATGTATACGCTGAAACAAACTTAGAAATTATTCATAGTATG
GTTCAAAAAGAAAAGCCAGATGTATTAGTAATAGATTCAATACAAACTATATACCATCCAGAAGTTACGTCGGCTCCTGG
TTCAGTATCTCAAGTGAGAGAATGTACACAGAATTTAATGCATATTGCTAAGCAAATGAATATAGCAACATTCATCGTTG
GACATGTTACGAAAGATGGACAAATTGCAGGTCCTAGATTGCTAGAACACATGGTGGATACCGTATTATATTTTGAAGGG
GACACACATCACGCTTATCGAATATTAAGAGCAGTTAAAAACCGTTTTGGTTCAACAAATGAAATGGGTATATTTGAAAT
GAAACAAACTGGCTTAAACGAAGTGAAAAATCCTTCGGAAATGTTCCTAGAAGAAAGAACAAAACATGTGGCTGGCTCGA
CTATTGTTGCAACTATGGAAGGAACAAGACCGATATTAGTTGAAGTACAGGCATTAGTAACGCCAACAACTTTCCATAAT
CCAAGAAGGATGGCTACTGGTATAGACCATAATAGATTGAGCTTGTTGATGGCAGTACTTGAAAAGAAAGAAAATTTCTT
ACTACAGCAACAAGATGCATATATTAAAATCGCTGGTGGTGTTAAATTAAGTGAGCCAGCAGTTGACTTAAGTATTATAA
TAGCAATAGCATCTAGTTTTCAGGATAAACCAACAAAAGGAGACGATTGTTTTGTTGGTGAAGTCGGCTTAACTGGAGAA
GTAAGAAGGGTTGCTAGAATTGACCAACGTGTTCAGGAAGCGGCTAAACTAGGTTTTAAACGCATCATCGTACCGAAAAA
TAATTTAAAAGGTTTAGAGTTCCCAGAAGGCACACAAGTTATTGGTGTTTCTACAACAAGAGAAGCATTGAAAATTGCCT
TTAATGCATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(349-428)

AAA domain (AAA_25)

(64-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.502

100

0.658
  radA Streptococcus pneumoniae Rx1

62.115

99.561

0.618
  radA Streptococcus pneumoniae D39

62.115

99.561

0.618
  radA Streptococcus pneumoniae R6

62.115

99.561

0.618
  radA Streptococcus pneumoniae TIGR4

62.115

99.561

0.618
  radA Streptococcus mitis NCTC 12261

62.115

99.561

0.618
  radA Streptococcus mitis SK321

62.115

99.561

0.618