Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PYH63_RS12300 Genome accession   NZ_CP118788
Coordinates   2542485..2543855 (-) Length   456 a.a.
NCBI ID   WP_353421391.1    Uniprot ID   -
Organism   Staphylococcus delphini strain 7070     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2537485..2548855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYH63_RS12275 (PYH63_12190) cysE 2537652..2538317 (-) 666 WP_353421941.1 serine O-acetyltransferase -
  PYH63_RS12280 (PYH63_12195) gltX 2538703..2540157 (-) 1455 WP_096647858.1 glutamate--tRNA ligase -
  PYH63_RS12285 (PYH63_12200) ispF 2540234..2540713 (-) 480 WP_262610668.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PYH63_RS12290 (PYH63_12205) ispD 2540706..2541404 (-) 699 WP_096597816.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  PYH63_RS12295 (PYH63_12210) - 2541405..2542466 (-) 1062 WP_019166688.1 PIN/TRAM domain-containing protein -
  PYH63_RS12300 (PYH63_12215) radA 2542485..2543855 (-) 1371 WP_353421391.1 DNA repair protein RadA Machinery gene
  PYH63_RS12305 (PYH63_12220) - 2544126..2546585 (-) 2460 WP_019166690.1 ATP-dependent Clp protease ATP-binding subunit -
  PYH63_RS12310 (PYH63_12225) - 2546601..2547605 (-) 1005 WP_019166691.1 protein arginine kinase -
  PYH63_RS12315 (PYH63_12230) - 2547595..2548125 (-) 531 WP_262610675.1 UvrB/UvrC motif-containing protein -
  PYH63_RS12320 (PYH63_12235) - 2548140..2548601 (-) 462 WP_014614779.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49992.47 Da        Isoelectric Point: 7.2142

>NTDB_id=795268 PYH63_RS12300 WP_353421391.1 2542485..2543855(-) (radA) [Staphylococcus delphini strain 7070]
MAKTKTIFECTACGYQSPKWMGKCPNCGAWNSMEESFEQKTASPKHGVRAQKTSSAKIQKLNDIKQEFAPRIQTNSGELN
RVLGGGIVEGSLVLIGGDPGIGKSTLLLQMCAALSKEKRVLYITGEESLNQTKIRADRLDEDASQLNVFAETDLMVIHEA
VKKVEPDLIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNVATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKSVLNPSEMFLEERTTNVAGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLGIVIATASSFKDQAVDGLDCYIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWQFPEGIEVIGVTSVNEALKHALKN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=795268 PYH63_RS12300 WP_353421391.1 2542485..2543855(-) (radA) [Staphylococcus delphini strain 7070]
GTGGCAAAAACGAAAACGATTTTTGAATGTACGGCCTGTGGTTACCAATCACCTAAATGGATGGGTAAATGTCCAAATTG
TGGTGCTTGGAATTCAATGGAGGAATCTTTTGAACAAAAAACTGCGAGTCCTAAACACGGTGTAAGGGCGCAAAAGACAA
GTTCGGCTAAAATACAAAAGCTCAATGACATTAAACAAGAGTTTGCGCCACGTATCCAAACGAATAGTGGTGAATTAAAC
CGTGTGCTCGGTGGCGGTATTGTAGAAGGGTCACTCGTGCTGATTGGTGGCGATCCAGGGATAGGGAAGTCGACACTGTT
ACTGCAAATGTGTGCGGCGTTATCTAAAGAAAAAAGAGTACTCTATATTACTGGTGAGGAATCACTCAATCAAACTAAAA
TTAGAGCGGATCGTTTAGATGAAGATGCGAGTCAACTGAACGTATTTGCGGAAACGGACTTAATGGTTATTCATGAAGCG
GTGAAAAAGGTCGAGCCTGATTTGATCGTCGTGGACTCGATTCAAACGATTTATCATCCTGAAATTAGTTCGGCGCCGGG
CTCTGTGTCGCAAGTTCGTGAAAGTACGCAAAGCTTGATGCATATCGCAAAGCAGATGAATGTTGCGACGTTTATTGTAG
GCCATGTGACGAAAGAAGGCCAAATTGCGGGGCCGCGTTTGCTGGAACATATGGTGGATACGGTGCTTTATTTTGAAGGT
GATGAACATCACGCGTATCGTATTTTAAGAGCGGTGAAAAACCGTTTTGGCTCTACGAATGAAATGGGCATTTTTGAAAT
GAAGCAGTCCGGTTTAAAAAGTGTGCTCAATCCGTCAGAAATGTTTTTAGAAGAACGGACGACCAATGTTGCAGGTTCGA
CAATTGTTGCAACGATGGAAGGAACACGACCATTATTGATTGAAGTACAAGCGCTTGTGACACCGACAACGTTCAATAAC
CCGAGACGTATGGCGACAGGTATTGACCATAATCGCCTCAGTTTATTGATGGCGGTGTTAGAGAAAAAAGAAGGGTATTT
ACTACAACAACAAGATGCTTACATTAAAGTAGCAGGGGGCGTCAAACTTTCTGAACCTGCTGTCGACTTAGGTATCGTCA
TTGCGACAGCTTCTAGTTTTAAAGACCAAGCAGTAGACGGCCTCGATTGCTATATTGGCGAGGTTGGATTGACGGGTGAG
GTGCGCCGTGTTTCACGTATTGAGCAACGTGTGCAAGAAGCGGCAAAACTGGGCTTTAAACGCGTAATTATTCCTAAAAA
TAATATTGGCGGATGGCAGTTCCCAGAAGGTATTGAAGTGATTGGCGTGACTTCTGTCAATGAAGCATTGAAACACGCAT
TAAAAAATTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(345-428)

AAA domain (AAA_25)

(72-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.789

100

0.658
  radA Streptococcus pneumoniae Rx1

58.242

99.781

0.581
  radA Streptococcus pneumoniae D39

58.242

99.781

0.581
  radA Streptococcus pneumoniae R6

58.242

99.781

0.581
  radA Streptococcus pneumoniae TIGR4

58.242

99.781

0.581
  radA Streptococcus mitis NCTC 12261

58.242

99.781

0.581
  radA Streptococcus mitis SK321

58.242

99.781

0.581