Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PYH61_RS12335 Genome accession   NZ_CP118776
Coordinates   2451229..2452599 (-) Length   456 a.a.
NCBI ID   WP_016999403.1    Uniprot ID   A0AAP1RP12
Organism   Mammaliicoccus lentus strain 7074     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2446229..2457599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYH61_RS12310 (PYH61_12310) cysE 2446623..2447264 (-) 642 WP_103268660.1 serine O-acetyltransferase -
  PYH61_RS12315 (PYH61_12315) gltX 2447515..2448972 (-) 1458 WP_147640581.1 glutamate--tRNA ligase -
  PYH61_RS12320 (PYH61_12320) ispF 2448989..2449468 (-) 480 WP_016999406.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PYH61_RS12325 (PYH61_12325) ispD 2449461..2450147 (-) 687 WP_103268658.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  PYH61_RS12330 (PYH61_12330) - 2450149..2451207 (-) 1059 WP_103268657.1 PIN/TRAM domain-containing protein -
  PYH61_RS12335 (PYH61_12335) radA 2451229..2452599 (-) 1371 WP_016999403.1 DNA repair protein RadA Machinery gene
  PYH61_RS12340 (PYH61_12340) - 2452755..2455220 (-) 2466 WP_016999402.1 ATP-dependent Clp protease ATP-binding subunit -
  PYH61_RS12345 (PYH61_12345) - 2455240..2456247 (-) 1008 WP_016999401.1 protein arginine kinase -
  PYH61_RS12350 (PYH61_12350) - 2456249..2456785 (-) 537 WP_016999400.1 UvrB/UvrC motif-containing protein -
  PYH61_RS12355 (PYH61_12355) - 2456800..2457261 (-) 462 WP_016999399.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50489.29 Da        Isoelectric Point: 7.7094

>NTDB_id=795175 PYH61_RS12335 WP_016999403.1 2451229..2452599(-) (radA) [Mammaliicoccus lentus strain 7074]
MAKKKVIFECLACGYQSPKWMGKCPNCGAWNQMEETIEHKETKGPKNTFKSDVPAQKIEKLKDIKDEDTPRILTKINEFN
RVLGGGVVEGSLVLIGGDPGIGKSTLLLQICAALSQQKNVLYVSGEESVRQTKLRAERLVEDSGELNVYAETNLEIIHSM
VQKEKPDVLVIDSIQTIYHPEVTSAPGSVSQVRECTQNLMHIAKQMNIATFIVGHVTKDGQIAGPRLLEHMVDTVLYFEG
DTHHAYRILRAVKNRFGSTNEMGIFEMKQTGLNEVKNPSEMFLEERTKHVAGSTIVATMEGTRPILVEVQALVTPTTFHN
PRRMATGIDHNRLSLLMAVLEKKENFLLQQQDAYIKIAGGVKLSEPAVDLSIIIAIASSFQDKPTKGDDCFVGEVGLTGE
VRRVARIDQRVQEAAKLGFKRIIVPKNNLKGLEFPEGTQVIGVSTTREALKIAFNA

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=795175 PYH61_RS12335 WP_016999403.1 2451229..2452599(-) (radA) [Mammaliicoccus lentus strain 7074]
ATGGCGAAAAAGAAAGTAATATTTGAATGTTTAGCCTGTGGGTACCAATCGCCAAAATGGATGGGCAAATGTCCTAATTG
TGGCGCATGGAATCAAATGGAAGAAACAATAGAACATAAAGAAACAAAAGGGCCTAAAAATACATTTAAATCAGATGTAC
CAGCACAAAAAATAGAAAAATTAAAAGACATTAAAGATGAAGACACACCTAGAATTTTAACGAAAATTAATGAATTTAAC
CGCGTATTAGGCGGCGGAGTAGTAGAAGGGTCGCTTGTATTAATAGGTGGAGATCCGGGTATCGGTAAATCGACACTATT
ATTACAAATTTGTGCCGCTCTATCACAACAGAAAAACGTACTTTACGTCTCTGGTGAGGAATCGGTAAGACAAACTAAAT
TAAGAGCAGAAAGACTCGTTGAAGATTCAGGCGAACTAAATGTATACGCTGAAACAAACTTAGAAATTATTCATAGTATG
GTTCAAAAAGAAAAGCCAGATGTATTAGTAATAGATTCAATACAAACTATATACCATCCAGAAGTTACGTCGGCTCCTGG
TTCAGTATCTCAAGTGAGAGAATGTACACAGAATTTAATGCATATTGCTAAGCAAATGAATATAGCAACATTCATCGTTG
GACATGTTACGAAAGATGGACAAATTGCAGGTCCTAGATTGCTAGAACACATGGTGGATACCGTATTATATTTTGAAGGG
GACACACATCACGCTTATCGAATATTAAGAGCAGTTAAAAACCGTTTTGGTTCAACAAATGAAATGGGTATATTTGAAAT
GAAACAAACTGGCTTAAACGAAGTGAAAAATCCTTCGGAAATGTTCCTAGAAGAAAGAACAAAACATGTGGCTGGCTCGA
CTATTGTTGCAACTATGGAAGGAACAAGACCGATATTAGTTGAAGTACAGGCATTAGTAACGCCAACAACTTTCCATAAT
CCAAGAAGGATGGCTACTGGTATAGACCATAATAGATTGAGCTTGTTGATGGCAGTACTTGAAAAGAAAGAAAATTTCTT
ACTACAGCAACAAGATGCATATATTAAAATCGCTGGTGGTGTTAAATTAAGTGAGCCAGCAGTTGACTTAAGTATTATAA
TAGCAATAGCATCTAGTTTTCAGGATAAACCAACAAAAGGAGACGATTGTTTTGTTGGTGAAGTCGGCTTAACTGGAGAA
GTAAGAAGGGTTGCTAGAATTGACCAACGTGTTCAGGAAGCGGCTAAACTAGGTTTTAAACGCATCATCGTACCGAAAAA
TAATTTAAAAGGTTTAGAGTTCCCAGAAGGCACACAAGTTATTGGTGTTTCTACAACAAGAGAAGCATTGAAAATTGCCT
TTAATGCATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(349-428)

AAA domain (AAA_25)

(64-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.502

100

0.658
  radA Streptococcus pneumoniae Rx1

62.335

99.561

0.621
  radA Streptococcus pneumoniae D39

62.335

99.561

0.621
  radA Streptococcus pneumoniae R6

62.335

99.561

0.621
  radA Streptococcus pneumoniae TIGR4

62.335

99.561

0.621
  radA Streptococcus mitis NCTC 12261

62.335

99.561

0.621
  radA Streptococcus mitis SK321

62.335

99.561

0.621