Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PW274_RS00120 Genome accession   NZ_CP118775
Coordinates   23018..24634 (+) Length   538 a.a.
NCBI ID   WP_016454603.1    Uniprot ID   A0AAX3SLT0
Organism   Delftia tsuruhatensis strain ULwDis3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18018..29634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PW274_RS00095 (PW274_00095) argS 18195..19892 (+) 1698 WP_016450693.1 arginine--tRNA ligase -
  PW274_RS00100 (PW274_00100) - 19913..20602 (+) 690 WP_043790827.1 SPOR domain-containing protein -
  PW274_RS00105 (PW274_00105) - 20684..21334 (+) 651 WP_016450695.1 thiol:disulfide interchange protein DsbA/DsbL -
  PW274_RS00110 (PW274_00110) lptA 21507..22169 (+) 663 WP_016454602.1 lipopolysaccharide transport periplasmic protein LptA -
  PW274_RS00115 (PW274_00115) lptB 22166..22963 (+) 798 WP_016450697.1 LPS export ABC transporter ATP-binding protein -
  PW274_RS00120 (PW274_00120) rpoN/rpoN1 23018..24634 (+) 1617 WP_016454603.1 RNA polymerase factor sigma-54 Machinery gene
  PW274_RS00125 (PW274_00125) rtcR 24656..26281 (-) 1626 WP_126312923.1 RNA repair transcriptional activator RtcR -
  PW274_RS00130 (PW274_00130) - 26979..28118 (+) 1140 WP_013804750.1 slipin family protein -
  PW274_RS00135 (PW274_00135) - 28175..28672 (+) 498 WP_016454605.1 hypothetical protein -

Sequence

Protein


Download         Length: 538 a.a.        Molecular weight: 59267.33 Da        Isoelectric Point: 4.5784

>NTDB_id=795128 PW274_RS00120 WP_016454603.1 23018..24634(+) (rpoN/rpoN1) [Delftia tsuruhatensis strain ULwDis3]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLGDNPFLELANDDGDHEAASSAPEPAAPGADAADSFQESR
ADQPSAAVEWEAPSDTSSGDAVETSGTAESLSWEGDGQTNSGNDDRDWGSDAPSSRTRGQGDDDETDATELARSAESLTD
HLHRQALSLRLGEVDRAALRFLIENLNDDGYLEDPLDELAQGLAGDDLEECEELVHRFTVALRLLQSLEPVGVGARDLAE
CLTLQLRDQLADAEREGDGSAEDEDRQAVLRTALDICAQPMDLLARRDLRKLASLCGAREERLREAMVLIARLEPRPGRR
FADVQRQIVVPDVIVRPVRMGAGRMEFSVQLNPDVMPKLRVHDIYASALRSHRGSEGHAALQQRLQEARWFIKNIQQRFD
TILRVSQAIVERQKNFFVHGELAMRPLVLRDIADALGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTEAGGNASST
AVRALIKQFVAAEDPRKPLSDNQLADMLKEQGIECARRTVAKYRELLKIPPTTLRKAL

Nucleotide


Download         Length: 1617 bp        

>NTDB_id=795128 PW274_RS00120 WP_016454603.1 23018..24634(+) (rpoN/rpoN1) [Delftia tsuruhatensis strain ULwDis3]
TTGAAGCCCGGACTGTCGCTGCGCGTCTCCCAGCATCTTTCGCTCACCCCGCAACTGCAGCAGTCGATACAGCTGCTGCA
GCTGTCCACGCTGGAGCTGGCGCAGGAAGTGGAGCAGATGCTGGGCGACAACCCCTTCCTGGAGCTGGCGAACGACGACG
GCGACCACGAAGCGGCAAGCAGCGCCCCGGAGCCTGCGGCGCCCGGCGCCGACGCCGCGGACTCCTTCCAGGAAAGCCGT
GCGGACCAGCCATCGGCTGCGGTGGAATGGGAGGCGCCGTCGGACACATCCTCCGGCGATGCCGTCGAGACCTCCGGCAC
GGCCGAGTCCCTGAGCTGGGAAGGCGATGGCCAGACCAACTCGGGCAACGACGACCGCGACTGGGGCAGCGATGCGCCTT
CCAGCCGCACGCGCGGCCAGGGTGACGATGACGAGACCGATGCCACCGAGCTGGCACGCAGCGCAGAAAGCCTGACCGAC
CACCTGCATCGCCAGGCCCTGAGCCTGCGCCTGGGCGAGGTGGATCGCGCCGCGCTGCGCTTTCTGATCGAGAACCTCAA
CGACGACGGCTACCTGGAGGACCCGCTCGACGAGCTGGCACAGGGCCTGGCGGGCGACGACCTGGAGGAGTGCGAGGAAC
TGGTGCACCGCTTCACCGTGGCGCTGCGCCTGTTGCAAAGCCTGGAGCCGGTGGGTGTGGGCGCGCGCGACCTGGCCGAA
TGCCTGACGCTGCAGCTGCGCGACCAACTGGCCGATGCCGAGCGCGAAGGCGACGGCAGTGCCGAGGACGAGGATCGGCA
GGCCGTGCTGCGCACCGCGCTGGACATCTGCGCCCAGCCCATGGACCTGCTGGCGCGGCGTGACCTGCGCAAGCTCGCCA
GCCTGTGCGGCGCGCGCGAGGAGCGGCTGCGCGAGGCGATGGTCCTGATCGCCCGGCTGGAGCCGCGCCCGGGCCGGCGC
TTCGCCGACGTGCAGCGCCAGATCGTCGTGCCCGATGTCATCGTGCGCCCGGTGCGCATGGGTGCGGGACGCATGGAGTT
CAGCGTGCAACTCAACCCCGATGTCATGCCCAAGCTGCGCGTGCACGACATCTATGCCAGCGCGCTGCGCAGCCACCGGG
GCAGCGAAGGCCATGCGGCGTTGCAGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGAC
ACCATCCTGCGCGTGTCGCAGGCCATCGTGGAGCGCCAGAAGAACTTCTTCGTGCATGGCGAGCTGGCCATGCGCCCGCT
GGTGCTGCGCGACATTGCCGATGCGCTGGGCCTGCATGAATCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCA
CGCCGCAGGGCACCTACGAACTCAAGTATTTCTTCGGCTCGGGCCTGGGCACCGAGGCGGGCGGCAACGCGTCGAGCACG
GCCGTGCGCGCGCTCATCAAGCAGTTCGTCGCAGCCGAGGACCCCAGGAAGCCGCTGTCGGACAACCAGCTCGCCGACAT
GCTCAAGGAGCAGGGCATCGAATGCGCGCGGCGCACGGTGGCCAAGTACCGCGAGCTGCTCAAGATCCCGCCGACCACGC
TGCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-365)

Sigma-54, DNA binding domain (Sigma54_DBD)

(380-536)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.111

100

0.513