Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PWO55_RS01200 Genome accession   NZ_CP118770
Coordinates   223156..223593 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain IMD4036     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 218156..228593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWO55_RS01160 (PWO55_01160) - 218736..218900 (-) 165 WP_370648487.1 molybdopterin dinucleotide binding domain-containing protein -
  PWO55_RS01165 (PWO55_01165) - 219014..220216 (+) 1203 WP_025853783.1 GTP-binding protein -
  PWO55_RS01170 (PWO55_01170) - 220281..221417 (+) 1137 WP_048368274.1 zinc-dependent alcohol dehydrogenase -
  PWO55_RS01175 (PWO55_01175) - 221432..221866 (+) 435 WP_021494203.1 RDD family protein -
  PWO55_RS01180 (PWO55_01180) - 221983..222258 (+) 276 WP_369719109.1 YckD family protein -
  PWO55_RS01185 - 222362..222445 (+) 84 WP_223151977.1 family 1 glycosylhydrolase -
  PWO55_RS01190 (PWO55_01185) - 222477..222695 (+) 219 Protein_219 family 1 glycosylhydrolase -
  PWO55_RS01195 (PWO55_01190) nin/comJ 222736..223134 (-) 399 WP_043866822.1 competence protein ComJ Regulator
  PWO55_RS01200 (PWO55_01195) nucA/comI 223156..223593 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  PWO55_RS01205 (PWO55_01200) hxlB 223952..224509 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  PWO55_RS01210 (PWO55_01205) hxlA 224506..225141 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  PWO55_RS01215 (PWO55_01210) - 225373..225735 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=795019 PWO55_RS01200 WP_003156588.1 223156..223593(-) (nucA/comI) [Bacillus velezensis strain IMD4036]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=795019 PWO55_RS01200 WP_003156588.1 223156..223593(-) (nucA/comI) [Bacillus velezensis strain IMD4036]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCAATAAAAGAAGGGCATTCCAGCATTTGCACCATTGATAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641