Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ORD17_RS07310 Genome accession   NZ_CP118747
Coordinates   1432452..1433873 (+) Length   473 a.a.
NCBI ID   WP_308387680.1    Uniprot ID   -
Organism   Acidithiobacillus sp. AMEEHan     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1427452..1438873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORD17_RS07270 - 1427794..1428456 (+) 663 WP_308387670.1 nucleotidyltransferase family protein -
  ORD17_RS07275 - 1428456..1428893 (+) 438 WP_308387671.1 hypothetical protein -
  ORD17_RS07280 - 1428922..1429185 (+) 264 WP_308387672.1 hypothetical protein -
  ORD17_RS07285 - 1429178..1430164 (+) 987 WP_308387673.1 KpsF/GutQ family sugar-phosphate isomerase -
  ORD17_RS07290 - 1430161..1430691 (+) 531 WP_308387674.1 HAD hydrolase family protein -
  ORD17_RS07295 lptC 1430688..1431233 (+) 546 WP_308387675.1 LPS export ABC transporter periplasmic protein LptC -
  ORD17_RS07300 lptA 1431223..1431726 (+) 504 WP_308387676.1 lipopolysaccharide transport periplasmic protein LptA -
  ORD17_RS07305 lptB 1431723..1432448 (+) 726 WP_308387678.1 LPS export ABC transporter ATP-binding protein -
  ORD17_RS07310 rpoN/rpoN1 1432452..1433873 (+) 1422 WP_308387680.1 RNA polymerase factor sigma-54 Machinery gene
  ORD17_RS07315 hpf 1433982..1434320 (+) 339 WP_308387682.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ORD17_RS07320 - 1434323..1434763 (+) 441 WP_308387683.1 PTS sugar transporter subunit IIA -
  ORD17_RS07325 hprK 1434818..1435735 (+) 918 WP_308387684.1 HPr(Ser) kinase/phosphatase -
  ORD17_RS07330 rapZ 1435732..1436631 (+) 900 WP_308387685.1 RNase adapter RapZ -
  ORD17_RS07335 - 1436628..1437011 (+) 384 WP_308387686.1 hypothetical protein -
  ORD17_RS07340 tal 1437075..1438163 (+) 1089 WP_308387687.1 transaldolase -

Sequence

Protein


Download         Length: 473 a.a.        Molecular weight: 52769.50 Da        Isoelectric Point: 4.4157

>NTDB_id=794965 ORD17_RS07310 WP_308387680.1 1432452..1433873(+) (rpoN/rpoN1) [Acidithiobacillus sp. AMEEHan]
MKQGLELKLGQHLAMTPQLQQAIRLLQLSTLDLQQEVQMMLDSNPLLDEEEGVELAASDASSPAPEERQLDLAAETSLPE
ELPVDSQWEDVFDMGSSASGSATANEEDLPDFEARNSREQSLQDYLRWQADLTFFQSDDRNLAELIIDAIDDTGYLGAEL
TELAETSGRPVAELEAILRQIQDFDPPGIAARSLAECLSLQLQQIEEEDDALLLARRIVDEQLELLAKHDYVRLAQALDA
REEDLRAAVALIAALNPKPGSEIGAGASEYVIPDVIVRWVGKRLRVELNAEAMPRLRINRHYADLSGGKDAAHRYIQDQL
NEARWFIKSLQSRQETILRVATAIVEQQKDFFLHGPEAMRPMVLRDIAEAVEMHESTISRVTNQKFMTTPRGLFEFKYFF
SSHVGTDQGGAASATAIRAVILKMVQAEDSRHPLSDAEIAKILAEQGIQIARRTVAKYREAANIPPAGQRRRL

Nucleotide


Download         Length: 1422 bp        

>NTDB_id=794965 ORD17_RS07310 WP_308387680.1 1432452..1433873(+) (rpoN/rpoN1) [Acidithiobacillus sp. AMEEHan]
ATGAAACAAGGACTCGAACTCAAACTCGGCCAACATCTGGCCATGACTCCACAATTGCAGCAGGCGATTCGCCTTCTGCA
ATTGTCGACCCTGGACCTGCAGCAGGAAGTGCAGATGATGCTCGACAGCAACCCGCTCCTCGATGAAGAGGAAGGCGTGG
AGCTGGCGGCGAGCGATGCTTCATCTCCAGCGCCGGAGGAGCGTCAGCTCGATCTCGCGGCGGAGACCAGCCTGCCCGAG
GAGCTGCCTGTCGACAGCCAGTGGGAAGATGTGTTCGATATGGGCAGCAGCGCCTCCGGAAGCGCGACGGCCAATGAGGA
GGATCTGCCCGATTTCGAGGCGCGCAACAGCCGCGAACAAAGCCTGCAGGACTACCTTCGCTGGCAGGCTGACCTCACCT
TTTTCCAGAGTGATGATCGCAATCTGGCCGAGTTGATCATCGATGCCATCGACGATACCGGCTATCTGGGGGCGGAATTG
ACTGAGTTGGCGGAGACCTCTGGTCGACCTGTGGCGGAGCTGGAAGCGATTCTGCGACAGATCCAGGATTTTGATCCGCC
AGGGATCGCTGCCCGCAGTCTGGCAGAGTGCCTCTCCTTGCAGTTGCAGCAGATCGAGGAAGAAGATGATGCCCTGCTGC
TCGCCCGCCGCATCGTCGATGAGCAGCTCGAGCTTTTGGCCAAGCATGACTATGTCCGTCTGGCGCAGGCTCTGGATGCG
CGCGAGGAGGATTTGCGCGCTGCCGTCGCCCTGATTGCTGCCCTCAATCCCAAACCCGGCAGCGAAATTGGTGCCGGAGC
CTCGGAATATGTCATTCCCGATGTCATCGTGCGTTGGGTAGGCAAACGGCTGCGAGTGGAACTGAACGCCGAGGCGATGC
CGCGGCTGCGCATCAATCGTCATTACGCTGACCTGTCCGGTGGCAAGGACGCCGCGCACCGATACATCCAGGATCAGCTC
AATGAGGCGCGCTGGTTCATCAAAAGTCTGCAAAGCCGGCAAGAAACCATCCTGAGGGTAGCTACGGCGATCGTTGAACA
GCAGAAGGACTTTTTCCTGCACGGACCGGAGGCAATGCGTCCCATGGTTCTGCGCGATATTGCCGAGGCTGTGGAGATGC
ATGAATCGACGATCTCCCGGGTGACCAATCAAAAGTTCATGACCACCCCGCGCGGCTTGTTCGAATTCAAATATTTTTTC
TCCAGTCATGTCGGTACGGATCAGGGTGGGGCGGCATCTGCCACCGCCATCCGCGCCGTCATTCTCAAAATGGTTCAGGC
GGAGGATAGTCGGCATCCTCTCAGCGATGCGGAAATCGCCAAGATACTTGCCGAGCAGGGCATCCAGATTGCCCGTCGTA
CGGTCGCCAAGTACCGCGAGGCCGCCAACATCCCGCCAGCCGGCCAGCGCCGCCGCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(112-302)

Sigma-54, DNA binding domain (Sigma54_DBD)

(314-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.703

100

0.495