Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PWG69_RS10145 Genome accession   NZ_CP118744
Coordinates   2004367..2004795 (+) Length   142 a.a.
NCBI ID   WP_025809943.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain RP01     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1993992..2010772 2004367..2004795 within 0


Gene organization within MGE regions


Location: 1993992..2010772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWG69_RS10080 (PWG69_10080) - 1993992..1994855 (+) 864 WP_023857602.1 manganese catalase family protein -
  PWG69_RS10085 (PWG69_10085) - 1994938..1995738 (+) 801 WP_020451579.1 DUF2935 domain-containing protein -
  PWG69_RS10090 (PWG69_10090) - 1995870..1996352 (+) 483 WP_025809932.1 GNAT family N-acetyltransferase -
  PWG69_RS10095 (PWG69_10095) - 1996369..1996671 (+) 303 WP_020451581.1 hypothetical protein -
  PWG69_RS10100 (PWG69_10100) - 1996995..1997600 (+) 606 WP_020451582.1 TetR/AcrR family transcriptional regulator -
  PWG69_RS10105 (PWG69_10105) - 1997840..1998295 (+) 456 WP_020451583.1 MarR family winged helix-turn-helix transcriptional regulator -
  PWG69_RS10110 (PWG69_10110) - 1998665..1999603 (+) 939 WP_025809935.1 ring-cleaving dioxygenase -
  PWG69_RS10115 (PWG69_10115) - 1999625..2000233 (+) 609 WP_025809937.1 flavin reductase family protein -
  PWG69_RS10120 (PWG69_10120) wrbA 2000435..2001049 (+) 615 WP_020451586.1 NAD(P)H:quinone oxidoreductase -
  PWG69_RS10125 (PWG69_10125) - 2001359..2001499 (+) 141 WP_023857597.1 hypothetical protein -
  PWG69_RS10130 (PWG69_10130) - 2001492..2002325 (+) 834 WP_020451587.1 prohibitin family protein -
  PWG69_RS10135 (PWG69_10135) - 2002414..2003175 (+) 762 WP_025809940.1 hypothetical protein -
  PWG69_RS10140 (PWG69_10140) - 2003235..2004074 (+) 840 WP_025809941.1 thymidylate synthase -
  PWG69_RS10145 (PWG69_10145) nucA/comI 2004367..2004795 (+) 429 WP_025809943.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  PWG69_RS10150 (PWG69_10150) lexA 2004994..2005617 (-) 624 WP_020451591.1 transcriptional repressor LexA -
  PWG69_RS10155 (PWG69_10155) yneA 2005769..2006080 (+) 312 WP_020451592.1 cell division suppressor protein YneA -
  PWG69_RS10160 (PWG69_10160) - 2006099..2006746 (+) 648 WP_020451593.1 YneB family resolvase-like protein -
  PWG69_RS10165 (PWG69_10165) - 2006812..2007045 (+) 234 WP_020451594.1 DUF896 domain-containing protein -
  PWG69_RS10170 (PWG69_10170) tkt 2007209..2009212 (+) 2004 WP_020451595.1 transketolase -
  PWG69_RS10175 (PWG69_10175) - 2009342..2010772 (+) 1431 WP_020451077.1 IS1182 family transposase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15404.40 Da        Isoelectric Point: 8.4717

>NTDB_id=794893 PWG69_RS10145 WP_025809943.1 2004367..2004795(+) (nucA/comI) [Bacillus paralicheniformis strain RP01]
MIKKWAIHLLFSALVLLGLSGGAAYSPQHAEGVARYDDVLYFPASRYPETGAHISDAIKAGHSDVCTIERSGADKRRQES
LKGIPTKPGFDRDEWPMAMCEEGGKGASVRYVSSSDNRGAGSWVGNRLSGYADGTRILFIVR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=794893 PWG69_RS10145 WP_025809943.1 2004367..2004795(+) (nucA/comI) [Bacillus paralicheniformis strain RP01]
ATGATCAAAAAATGGGCGATTCATCTGCTGTTTTCCGCATTGGTGCTGCTTGGGCTTTCGGGAGGGGCTGCATATTCTCC
TCAGCATGCCGAAGGCGTTGCAAGGTATGATGACGTATTGTATTTTCCGGCATCGCGCTATCCTGAAACAGGTGCTCATA
TAAGCGATGCGATCAAAGCGGGCCATTCAGATGTCTGCACAATTGAAAGATCTGGAGCGGATAAGCGCCGTCAGGAATCA
TTAAAGGGGATTCCGACTAAGCCGGGCTTTGACCGTGACGAATGGCCGATGGCCATGTGTGAAGAAGGGGGAAAAGGAGC
GTCGGTCAGATATGTCAGCTCATCGGATAACCGCGGAGCCGGCTCCTGGGTCGGGAACAGGCTGAGCGGTTACGCTGACG
GGACGAGAATTTTGTTTATCGTTCGATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.73

88.732

0.521