Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PWP91_RS12335 Genome accession   NZ_CP118738
Coordinates   2463737..2465098 (-) Length   453 a.a.
NCBI ID   WP_274998606.1    Uniprot ID   -
Organism   Lactococcus lactis strain P-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2458737..2470098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWP91_RS12300 (PWP91_12300) - 2459282..2459776 (+) 495 WP_012898594.1 GNAT family N-acetyltransferase -
  PWP91_RS12305 (PWP91_12305) - 2459869..2460192 (-) 324 WP_017865144.1 DUF1048 domain-containing protein -
  PWP91_RS12310 (PWP91_12310) - 2460179..2460526 (-) 348 WP_058204407.1 DUF1048 domain-containing protein -
  PWP91_RS12315 (PWP91_12315) - 2460513..2460875 (-) 363 WP_003129889.1 PadR family transcriptional regulator -
  PWP91_RS12320 (PWP91_12320) - 2460997..2461626 (-) 630 WP_023188601.1 hypothetical protein -
  PWP91_RS12325 (PWP91_12325) - 2461691..2462776 (-) 1086 WP_012898597.1 PIN/TRAM domain-containing protein -
  PWP91_RS12330 (PWP91_12330) - 2463007..2463693 (+) 687 WP_338084801.1 YoaK family protein -
  PWP91_RS12335 (PWP91_12335) radA 2463737..2465098 (-) 1362 WP_274998606.1 DNA repair protein RadA Machinery gene
  PWP91_RS12340 (PWP91_12340) - 2465260..2465487 (-) 228 WP_058204409.1 heavy-metal-associated domain-containing protein -
  PWP91_RS12350 (PWP91_12350) rplQ 2466205..2466585 (-) 381 WP_003130558.1 50S ribosomal protein L17 -
  PWP91_RS12355 (PWP91_12355) - 2466604..2467542 (-) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  PWP91_RS12360 (PWP91_12360) rpsK 2467590..2467973 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  PWP91_RS12365 (PWP91_12365) rpsM 2467994..2468359 (-) 366 WP_003130555.1 30S ribosomal protein S13 -
  PWP91_RS12370 (PWP91_12370) rpmJ 2468377..2468493 (-) 117 WP_001808836.1 50S ribosomal protein L36 -
  PWP91_RS12375 (PWP91_12375) infA 2468547..2468765 (-) 219 WP_003130554.1 translation initiation factor IF-1 -
  PWP91_RS12380 (PWP91_12380) - 2469062..2469709 (-) 648 WP_274998607.1 adenylate kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49878.27 Da        Isoelectric Point: 5.0754

>NTDB_id=794823 PWP91_RS12335 WP_274998606.1 2463737..2465098(-) (radA) [Lactococcus lactis strain P-1]
MAKIKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=794823 PWP91_RS12335 WP_274998606.1 2463737..2465098(-) (radA) [Lactococcus lactis strain P-1]
ATGGCGAAGATAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACGGACCTAGATGAATTTAATCGTGTA
TTAGGAGGGGGCGTCGTTCCTGGTTCTTTGGTTCTGATTGGGGGAGATCCTGGGATAGGTAAATCAACTTTACTTTTACA
GGTTTCCACTCAATTGGCTTCTAGAGGGCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACTTTTATTGTTGGTCATG
TGACTAAAGAAGGACAGCTGGCTGGGCCGAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
TCATGGCTTGGTCGAAGTGACTAACCCCTCAGAGGTCTTTTTAGAAGAACGCTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCACTTGAAGGAACACGGCCTATACTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAAAAGAGAACGGGGCTTTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGTTTTAAAAAAGTATATGCTCCAAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTGACAACTTTAACTGAATGTTTAAAACTGGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.015

100

0.81
  radA Streptococcus pneumoniae D39

81.015

100

0.81
  radA Streptococcus pneumoniae R6

81.015

100

0.81
  radA Streptococcus pneumoniae TIGR4

81.015

100

0.81
  radA Streptococcus mitis SK321

81.015

100

0.81
  radA Streptococcus mitis NCTC 12261

80.795

100

0.808
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62