Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PWO00_RS23220 Genome accession   NZ_CP118718
Coordinates   4455360..4456412 (-) Length   350 a.a.
NCBI ID   WP_275036495.1    Uniprot ID   -
Organism   Priestia aryabhattai strain G5MAi6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4450360..4461412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWO00_RS23180 (PWO00_23175) - 4451376..4452200 (+) 825 WP_045295433.1 YqhG family protein -
  PWO00_RS23185 (PWO00_23180) - 4452224..4452409 (-) 186 WP_013059170.1 YqzE family protein -
  PWO00_RS23190 (PWO00_23185) comGG 4452453..4452827 (-) 375 WP_221814281.1 competence type IV pilus minor pilin ComGG -
  PWO00_RS23195 (PWO00_23190) comGF 4452817..4453254 (-) 438 WP_221814282.1 competence type IV pilus minor pilin ComGF -
  PWO00_RS23200 (PWO00_23195) - 4453251..4453589 (-) 339 WP_216143616.1 type II secretion system protein -
  PWO00_RS23205 (PWO00_23200) comGD 4453573..4454010 (-) 438 WP_221814283.1 competence type IV pilus minor pilin ComGD -
  PWO00_RS23210 (PWO00_23205) comGC 4453997..4454308 (-) 312 WP_193424339.1 competence type IV pilus major pilin ComGC -
  PWO00_RS23215 (PWO00_23210) comGB 4454323..4455369 (-) 1047 WP_221814284.1 competence type IV pilus assembly protein ComGB -
  PWO00_RS23220 (PWO00_23215) comGA 4455360..4456412 (-) 1053 WP_275036495.1 competence type IV pilus ATPase ComGA Machinery gene
  PWO00_RS23225 (PWO00_23220) - 4456682..4457065 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PWO00_RS23230 (PWO00_23225) - 4457327..4457563 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  PWO00_RS23235 (PWO00_23230) - 4457601..4458233 (-) 633 WP_216143614.1 MBL fold metallo-hydrolase -
  PWO00_RS23240 (PWO00_23235) - 4458443..4458619 (+) 177 WP_028411614.1 DUF2759 domain-containing protein -
  PWO00_RS23245 (PWO00_23240) - 4458655..4459557 (-) 903 WP_221814285.1 M14 family metallopeptidase -
  PWO00_RS23250 (PWO00_23245) - 4459669..4460637 (-) 969 WP_013059183.1 ROK family glucokinase -
  PWO00_RS23255 (PWO00_23250) - 4460640..4460846 (-) 207 WP_028411617.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39613.01 Da        Isoelectric Point: 9.0586

>NTDB_id=794559 PWO00_RS23220 WP_275036495.1 4455360..4456412(-) (comGA) [Priestia aryabhattai strain G5MAi6]
MTEIEQFATYVIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSVDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHLACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=794559 PWO00_RS23220 WP_275036495.1 4455360..4456412(-) (comGA) [Priestia aryabhattai strain G5MAi6]
TTGACCGAAATTGAACAGTTTGCTACTTACGTTATACGAGATGCATGTGAGTGCCGAAGTTCAGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAGGAACTTATTTTAAAAAGGGTCATTTCAAAAGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGCGGAATGGATATTGGCGAACGCAGAAAACCTCAAAATGGTGCGTTGACA
ACTTCCGTTGATCACTATCAAGTAAATTTGCGTTTATCTACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTACCTCATCAACAGTCTACATCTCTCAAACATTTATCTCTGTTTCCTGAACATACAAAAAAGCTTATTTCTCTCTTGA
AATTCTCCCATGGATTAATTATTTTTACCGGCCCTACAGGATCGGGTAAAACCACTACTCTATACACTCTTTTACATGAA
GTAAAGCATCTTTTTAACCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTGCA
GGTGAATGAACGCGCAGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACATTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAGTTTGGCGTTAGCTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCACAGCGTCTTGTAGAAATTCGCTGTCCGCTGTGTTCTGGAAAATGCCACCTGGCTTGTAAAAAAATGAGGA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCGGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATTGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586