Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PWJ66_RS22180 Genome accession   NZ_CP118714
Coordinates   4464443..4465819 (-) Length   458 a.a.
NCBI ID   WP_008180607.1    Uniprot ID   -
Organism   Lysinibacillus fusiformis strain G5.M11b     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4459443..4470819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWJ66_RS22160 (PWJ66_22095) gltX 4460024..4461484 (-) 1461 WP_274993419.1 glutamate--tRNA ligase -
  PWJ66_RS22165 (PWJ66_22100) ispF 4461573..4462049 (-) 477 WP_036121644.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PWJ66_RS22170 (PWJ66_22105) ispD 4462434..4463129 (-) 696 WP_036121646.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  PWJ66_RS22175 (PWJ66_22110) - 4463141..4464226 (-) 1086 WP_036121647.1 PIN/TRAM domain-containing protein -
  PWJ66_RS22180 (PWJ66_22115) radA 4464443..4465819 (-) 1377 WP_008180607.1 DNA repair protein RadA Machinery gene
  PWJ66_RS22185 (PWJ66_22120) - 4465911..4468355 (-) 2445 WP_008180611.1 ATP-dependent Clp protease ATP-binding subunit -
  PWJ66_RS22190 (PWJ66_22125) - 4468345..4469454 (-) 1110 WP_036121652.1 protein arginine kinase -
  PWJ66_RS22195 (PWJ66_22130) - 4469451..4469993 (-) 543 WP_025116467.1 UvrB/UvrC motif-containing protein -
  PWJ66_RS22200 (PWJ66_22135) - 4470007..4470477 (-) 471 WP_150906497.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50197.03 Da        Isoelectric Point: 7.3825

>NTDB_id=794515 PWJ66_RS22180 WP_008180607.1 4464443..4465819(-) (radA) [Lysinibacillus fusiformis strain G5.M11b]
MAKKKTKFVCSGCGYESAKWMGRCPGCGEWNKMVEEVEVIAKGPRGAFQHSATVTQKAIPIIQVEASEESRVATEMGELN
RVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSALLSNKGHRVLYISGEESIRQTKLRAERLGVISQELYIYSETNLEFLNQ
TIEEVQPKFVIVDSIQTVFHPEVTSAPGSVSQVRECTAELMRIAKTKGIAIFLVGHVTKEGQIAGPRILEHMVDTVLYFE
GERHHNHRILRSQKNRFGSTNEIAIFEMLQGGLKEVLNPSELFLQERSQGAAGSTIVASMEGTRPILVEIQSLVTPTSFN
YPKRMATGVDQNRVQLLMAVLEKRMGLMLQAQDAYIKVAGGVKLDEPAIDLAVLTSIVSSFKDQAVRATDCFVGEVGLTG
EVRRVSRIEQRVIEAAKLGFKRVFIPTSNIGGWDFPQGIEIIGVETIKDALNACFREL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=794515 PWJ66_RS22180 WP_008180607.1 4464443..4465819(-) (radA) [Lysinibacillus fusiformis strain G5.M11b]
TTGGCAAAGAAAAAAACGAAATTTGTATGTTCAGGCTGTGGCTATGAATCAGCAAAATGGATGGGAAGATGTCCAGGGTG
TGGGGAATGGAATAAGATGGTGGAGGAAGTAGAAGTGATTGCGAAAGGACCACGTGGGGCTTTCCAGCATTCTGCCACTG
TTACTCAAAAGGCAATCCCTATCATTCAGGTGGAAGCTAGTGAAGAGTCCCGTGTAGCGACAGAAATGGGCGAGCTGAAC
CGTGTATTAGGCGGTGGTATTGTGCCTGGCTCTCTAGTATTAATAGGCGGTGATCCAGGGATTGGGAAATCCACTTTATT
ACTACAAGTTTCAGCCTTGCTTTCTAATAAAGGTCATCGTGTGCTGTATATTTCTGGTGAGGAATCTATCCGTCAAACGA
AGCTACGTGCGGAGCGTTTGGGGGTAATTTCGCAAGAGCTGTATATTTATTCTGAAACAAATCTTGAGTTTTTGAATCAA
ACGATTGAGGAAGTACAGCCGAAATTTGTCATTGTCGATTCCATACAAACCGTCTTTCATCCAGAGGTAACAAGTGCGCC
AGGTAGTGTCTCACAGGTCCGTGAGTGTACAGCTGAGCTTATGCGAATTGCTAAGACAAAAGGAATTGCGATCTTCTTAG
TTGGGCATGTTACGAAAGAGGGGCAAATAGCAGGGCCTCGTATTTTAGAGCATATGGTGGATACAGTCCTGTATTTTGAA
GGGGAAAGACATCATAATCATCGTATTTTACGAAGTCAAAAGAACCGCTTCGGTTCTACAAATGAAATTGCTATATTTGA
AATGCTACAGGGTGGGCTAAAAGAGGTTTTGAACCCATCTGAGCTGTTTTTACAGGAGCGTTCACAAGGAGCTGCGGGCT
CCACGATTGTTGCCTCAATGGAGGGAACAAGGCCAATACTCGTTGAAATCCAGTCACTCGTAACGCCTACCAGCTTTAAT
TACCCTAAGCGGATGGCAACAGGGGTTGATCAAAATAGAGTGCAATTACTGATGGCCGTACTTGAAAAGCGTATGGGTCT
CATGCTACAAGCGCAGGATGCATATATTAAAGTTGCTGGTGGTGTAAAATTGGACGAACCTGCCATCGATTTAGCTGTTC
TGACAAGCATCGTTTCTAGCTTTAAGGATCAGGCTGTGCGAGCAACGGATTGCTTTGTTGGTGAGGTAGGTCTGACAGGA
GAGGTGCGCCGAGTATCCCGTATTGAGCAACGTGTCATTGAAGCAGCGAAGCTTGGTTTTAAAAGAGTATTCATACCTAC
TTCCAATATAGGTGGATGGGATTTTCCACAAGGGATTGAAATTATCGGGGTAGAAACGATAAAGGATGCATTAAATGCCT
GCTTTAGAGAGTTATAG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(350-432)

AAA domain (AAA_25)

(66-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

70.925

99.127

0.703
  radA Streptococcus pneumoniae Rx1

63.158

99.563

0.629
  radA Streptococcus pneumoniae D39

63.158

99.563

0.629
  radA Streptococcus pneumoniae R6

63.158

99.563

0.629
  radA Streptococcus pneumoniae TIGR4

63.158

99.563

0.629
  radA Streptococcus mitis NCTC 12261

63.158

99.563

0.629
  radA Streptococcus mitis SK321

63.158

99.563

0.629