Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PWF74_RS10440 Genome accession   NZ_CP118627
Coordinates   2073343..2074704 (+) Length   453 a.a.
NCBI ID   WP_080567145.1    Uniprot ID   -
Organism   Lactococcus garvieae strain Pa-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2068343..2079704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWF74_RS10395 (PWF74_10395) - 2068800..2069522 (-) 723 WP_141921056.1 metal ABC transporter ATP-binding protein -
  PWF74_RS10400 (PWF74_10400) - 2069544..2069987 (-) 444 WP_014024192.1 zinc-dependent MarR family transcriptional regulator -
  PWF74_RS10405 (PWF74_10405) infA 2070189..2070407 (+) 219 WP_003133772.1 translation initiation factor IF-1 -
  PWF74_RS10410 (PWF74_10410) rpmJ 2070461..2070577 (+) 117 WP_014024193.1 50S ribosomal protein L36 -
  PWF74_RS10415 (PWF74_10415) rpsM 2070595..2070960 (+) 366 WP_003133773.1 30S ribosomal protein S13 -
  PWF74_RS10420 (PWF74_10420) rpsK 2070978..2071361 (+) 384 WP_003133774.1 30S ribosomal protein S11 -
  PWF74_RS10425 (PWF74_10425) - 2071413..2072351 (+) 939 WP_003133775.1 DNA-directed RNA polymerase subunit alpha -
  PWF74_RS10430 (PWF74_10430) rplQ 2072367..2072747 (+) 381 WP_003133776.1 50S ribosomal protein L17 -
  PWF74_RS10440 (PWF74_10440) radA 2073343..2074704 (+) 1362 WP_080567145.1 DNA repair protein RadA Machinery gene
  PWF74_RS10445 (PWF74_10445) - 2074838..2075914 (+) 1077 WP_165705519.1 PIN/TRAM domain-containing protein -
  PWF74_RS10450 (PWF74_10450) - 2075937..2076566 (+) 630 WP_165705520.1 pyridoxamine 5'-phosphate oxidase family protein -
  PWF74_RS10455 (PWF74_10455) ald 2076754..2077875 (+) 1122 WP_003133782.1 alanine dehydrogenase -
  PWF74_RS10460 (PWF74_10460) - 2077910..2078404 (-) 495 WP_014024197.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49768.92 Da        Isoelectric Point: 4.8676

>NTDB_id=794073 PWF74_RS10440 WP_080567145.1 2073343..2074704(+) (radA) [Lactococcus garvieae strain Pa-2]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGNGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARECFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVDIPENLEVVGVTTLSECLKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=794073 PWF74_RS10440 WP_080567145.1 2073343..2074704(+) (radA) [Lactococcus garvieae strain Pa-2]
ATAGCTAAAAAAAAATCAACATTTCTTTGCCAGGAATGTGGCTACAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGTGCTTGGGGTTCCTTTGTTGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGAGTGAGTCTGACAGGCGAGCACT
CTAAACCTATGAAGTTAGATCAGGTTGAACTTTTCGATACACCGCGTGTGGAAACCGATCTGGATGAGTTTAACCGCGTC
CTTGGAGGCGGTGTCGTTCCAGGAAGTCTTGTCCTTATTGGGGGGGATCCAGGGATTGGTAAATCTACTCTACTGCTTCA
AGTATCTACGCAACTTGCTTCAAGAGGGCGTGTACTTTATGTCAGTGGCGAGGAGTCGGCTCAACAAATTAAGCTGCGTG
CCGAGCGTTTGGGCGACATCGATACAGATTTTTACCTTTACGCCGAAACCAATATGCAAAGCATCCGAAATGAAGTTGAA
CGTCTACAGCCAGACTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATCCAGAGTACTCAGGGTTCGGT
TAGTCAGGTGCGCGAGGTGACAGGAGAATTAATGCAGTTGGCCAAAACCAACGATATTGCGACCTTTATTGTCGGACATG
TCACTAAGGAAGGGCAACTTGCGGGACCACGTATGTTGGAACATATGGTTGATACGGTCTTGTATTTTGAGGGAGAACGT
CAAAATACATTCCGAATTTTACGCGCGGTCAAAAACCGTTTTGGTTCAACAAATGAAATTGGGATTTTCGAGATGCAGGG
CAATGGTTTAGTGGAAGTGACCAACCCGAGTGAAGTTTTTCTTGAGGAGCGTTTGGAGGGCTCTACAGGCTCTGCTATCG
TCTGTGCGTTAGAAGGTACGCGTCCGATATTGGTTGAGATTCAAGCTTTGACAACGCCAACCATGTTTGGGAATGCCAAA
CGTACAACCTCTGGTTTGGACTTTAACCGTGTGAGCTTAATCATGGCAGTACTCGAAAAGCGTGCCGGTTTTCTTTTGCA
ACAACAAGATGCCTACTTAAAATCCGCAGGTGGTGTCAAGTTGGATGAACCAGCCATTGACTTAGCAGTCGCTGTAGCTA
TTGCTTCTAGCTACAAGGAAGAGCCGACAGATGCACGCGAATGCTTTATTGGCGAAATTGGCTTGACGGGTGAAATTCGT
CGTGTTACGCGGATGGAGCAACGTCTCAATGAAGCAAGCAAATTAGGGTTCCAAAAAGTTTACGCGCCTAAGAATAGCTT
GGCAGGCGTAGACATTCCAGAAAATTTGGAAGTGGTCGGTGTGACGACTTTATCAGAATGTTTGAAAAAAGTATTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.353

100

0.804
  radA Streptococcus pneumoniae D39

80.353

100

0.804
  radA Streptococcus pneumoniae R6

80.353

100

0.804
  radA Streptococcus pneumoniae TIGR4

80.353

100

0.804
  radA Streptococcus mitis NCTC 12261

80.353

100

0.804
  radA Streptococcus mitis SK321

80.353

100

0.804
  radA Bacillus subtilis subsp. subtilis str. 168

61.438

100

0.623