Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PWF74_RS01615 Genome accession   NZ_CP118627
Coordinates   315978..317783 (+) Length   601 a.a.
NCBI ID   WP_165705955.1    Uniprot ID   -
Organism   Lactococcus garvieae strain Pa-2     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 310978..322783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWF74_RS01595 (PWF74_01595) - 311135..311959 (-) 825 WP_014024379.1 Cof-type HAD-IIB family hydrolase -
  PWF74_RS01600 (PWF74_01600) - 312316..313737 (+) 1422 WP_014024380.1 NCS2 family permease -
  PWF74_RS01605 (PWF74_01605) - 313814..314914 (+) 1101 WP_165705957.1 YdcF family protein -
  PWF74_RS01610 (PWF74_01610) coiA 314990..315994 (+) 1005 WP_165705956.1 competence protein CoiA Machinery gene
  PWF74_RS01615 (PWF74_01615) pepF 315978..317783 (+) 1806 WP_165705955.1 oligoendopeptidase F Regulator
  PWF74_RS01620 (PWF74_01620) - 317783..318466 (+) 684 WP_165705954.1 O-methyltransferase -
  PWF74_RS01625 (PWF74_01625) - 318564..319472 (+) 909 WP_017370843.1 peptidyl-prolyl cis-trans isomerase -
  PWF74_RS01630 (PWF74_01630) alaS 319723..322341 (+) 2619 WP_165705953.1 alanine--tRNA ligase -

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 69839.49 Da        Isoelectric Point: 4.7057

>NTDB_id=794045 PWF74_RS01615 WP_165705955.1 315978..317783(+) (pepF) [Lactococcus garvieae strain Pa-2]
MAKKRDEIAENLTWDLSTVFENDAAWELELKRVSEKLETVNAYEGHLLDSAQSLLEITEMELEISRAVEKVYVYASMKHD
QDTQVSLYQEYEAKATNLYAKFGESFAFYEPEFLALTPERYESFRREEPKLERYSHMFERLFAKKEHILSPKEEKLLASA
SEIFGAAAETFEIFDNADIELPMIKDEKGQEVQLTHGNYISLMESKERKVRKAAYQALYSNYEQYQHTYAKTLQTNVKVH
NFKAKMRGYHSARQAALSSNFIPEEVYDKLLESVNKYLPLLHRYIKLREQILGLEDDLKMYDVYIPLSDLDYKFNYPEAV
EKAQEVLQILGPEYTHQVKKAFEERWIDVAENSGKRSGAYSGGCYDTNAFMLLNWQETLDDLFTLVHEMGHSMHSTFTRE
NQPYVYGDYPIFLAEIASTTNENILTESLLAASTDEKVRFAILNHWLDGFRGTVFRQSQFAEFEHEIHKADQEGKVLTSE
FMNQLYAELNEKYYGLPARENPEIQYEWARIPHFYYNYYVFQYATGFAAATYLAEAIVNGGTDAREKYLTYLKAGSSDYP
LAVIAQAGVDMEETTYLESAFAVFEKRLIELEKLVEKGAHL

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=794045 PWF74_RS01615 WP_165705955.1 315978..317783(+) (pepF) [Lactococcus garvieae strain Pa-2]
ATGGCAAAAAAACGTGATGAAATAGCTGAAAACTTAACTTGGGATTTGTCAACTGTATTTGAAAATGATGCCGCTTGGGA
ACTTGAACTTAAAAGAGTTTCGGAAAAATTAGAGACGGTAAACGCCTACGAAGGACATCTTCTAGATAGCGCTCAAAGCT
TATTAGAAATCACGGAAATGGAGCTAGAAATCTCGCGTGCTGTGGAAAAGGTTTATGTCTATGCCTCAATGAAGCATGAC
CAAGATACGCAAGTGAGCCTTTACCAAGAATACGAAGCCAAAGCCACAAATTTATATGCCAAGTTTGGTGAAAGCTTTGC
TTTTTATGAGCCGGAATTTTTAGCTTTAACGCCAGAACGTTATGAAAGTTTTAGAAGAGAAGAACCTAAACTGGAACGCT
ATTCCCATATGTTTGAACGCCTTTTTGCTAAAAAGGAGCATATTTTAAGTCCAAAAGAGGAAAAACTTTTAGCCAGTGCG
AGTGAGATTTTTGGTGCTGCGGCCGAAACCTTTGAAATTTTTGACAATGCTGATATTGAACTTCCGATGATTAAAGATGA
AAAAGGACAGGAAGTACAGCTGACACATGGCAATTATATCTCGCTCATGGAAAGTAAGGAGCGTAAGGTACGAAAGGCAG
CTTATCAAGCCCTTTATAGCAACTATGAACAGTATCAACACACTTATGCTAAAACGCTACAAACAAATGTAAAAGTCCAT
AATTTTAAGGCAAAGATGCGGGGATACCATTCAGCTCGTCAGGCAGCTTTATCAAGTAATTTTATTCCGGAAGAAGTCTA
CGATAAGCTCTTAGAGTCTGTCAATAAATATTTGCCTCTTTTACATCGCTATATTAAATTGCGTGAACAGATTTTAGGCT
TAGAAGACGATTTAAAGATGTATGACGTTTATATACCCTTATCGGACTTGGATTATAAGTTTAACTACCCCGAGGCAGTA
GAGAAAGCCCAAGAAGTTCTTCAAATTTTGGGGCCTGAATATACGCACCAAGTAAAAAAAGCTTTTGAGGAGCGCTGGAT
TGATGTGGCTGAAAATAGCGGTAAACGTTCGGGTGCTTACTCAGGTGGATGCTATGATACAAATGCTTTTATGCTGTTAA
ACTGGCAAGAAACTTTGGATGATTTGTTCACCTTAGTACATGAAATGGGGCACAGTATGCATTCCACATTTACACGGGAA
AATCAACCTTATGTTTATGGGGACTATCCAATCTTTCTTGCAGAAATTGCTTCAACAACCAATGAGAATATCTTGACTGA
AAGTTTACTTGCGGCCTCGACAGATGAAAAAGTGCGTTTTGCTATTTTAAACCACTGGCTTGATGGTTTCCGTGGCACTG
TTTTTCGTCAAAGTCAATTTGCTGAATTTGAACATGAAATACATAAAGCTGATCAAGAAGGAAAAGTTTTGACCAGTGAA
TTCATGAACCAGCTTTATGCAGAACTTAATGAAAAATACTATGGTTTACCCGCCCGGGAAAATCCTGAAATTCAGTATGA
GTGGGCCCGCATTCCGCACTTCTATTACAACTATTATGTCTTCCAATATGCTACTGGCTTTGCCGCAGCAACTTATCTTG
CTGAGGCTATCGTTAATGGTGGGACAGATGCGCGTGAAAAATACCTCACTTATCTCAAAGCCGGATCAAGTGATTATCCC
CTGGCTGTGATTGCTCAAGCAGGTGTGGATATGGAAGAAACAACATATTTAGAATCAGCTTTTGCTGTTTTTGAAAAACG
TTTAATTGAGCTAGAAAAATTAGTCGAAAAAGGAGCCCATTTATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(202-582)

Oligopeptidase F (Peptidase_M3_N)

(112-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

64.725

100

0.647