Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PWW23_RS15880 Genome accession   NZ_CP118586
Coordinates   3391323..3392546 (+) Length   407 a.a.
NCBI ID   WP_005446256.1    Uniprot ID   K5TX94
Organism   Vibrio harveyi strain fish6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3386323..3397546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW23_RS15860 ampD 3387341..3387892 (-) 552 WP_154203416.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWW23_RS15865 nadC 3387985..3388872 (+) 888 WP_199449848.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWW23_RS15870 - 3389137..3389604 (+) 468 WP_050933475.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PWW23_RS15875 pilB 3389604..3391289 (+) 1686 WP_005446257.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWW23_RS15880 pilC 3391323..3392546 (+) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PWW23_RS15885 pilD 3392619..3393488 (+) 870 WP_045488712.1 A24 family peptidase Machinery gene
  PWW23_RS15890 coaE 3393489..3394103 (+) 615 WP_050922069.1 dephospho-CoA kinase -
  PWW23_RS15895 zapD 3394131..3394871 (+) 741 WP_038897986.1 cell division protein ZapD -
  PWW23_RS15900 yacG 3394928..3395128 (+) 201 WP_026000212.1 DNA gyrase inhibitor YacG -
  PWW23_RS15905 rplS 3395694..3396047 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  PWW23_RS15910 trmD 3396089..3396832 (-) 744 WP_005446247.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  PWW23_RS15915 rimM 3396860..3397408 (-) 549 WP_005446245.1 ribosome maturation factor RimM -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44945.20 Da        Isoelectric Point: 10.3829

>NTDB_id=793715 PWW23_RS15880 WP_005446256.1 3391323..3392546(+) (pilC) [Vibrio harveyi strain fish6]
MKTIAPQLKNYRWKGINSSGKKTSGNVLAMTEVEVRERLDAQHIKIKKLKKGSISFITKISHRVKGKDITVFTRQIATML
MTGVPIVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASTHFDPLYTDLIATGEQSGNLSQVFERLATYREK
SEQLRAKVIKALIYPAMVVLVALGVSYLMLTKVIPEFEKMFTGFGAELPAFTQMVLNLSAWTQNWGPFIGLSFVSLLISG
KIMSQRSDSFRLLTARSSLRVPILGPVLSKAAIAKFSRTLATSFSAGIPILTALKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYIAMRNCNVFPELVLQMVMIGEESGRLDDMLNKIAAIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=793715 PWW23_RS15880 WP_005446256.1 3391323..3392546(+) (pilC) [Vibrio harveyi strain fish6]
ATGAAAACCATCGCTCCACAACTTAAGAACTACCGTTGGAAAGGCATTAATAGCTCGGGTAAAAAAACCTCTGGTAATGT
GCTCGCAATGACAGAAGTCGAGGTAAGAGAGCGCCTTGATGCTCAGCACATCAAGATAAAAAAACTCAAAAAAGGCAGTA
TTTCTTTTATCACCAAGATAAGTCATCGAGTAAAAGGCAAAGACATTACGGTGTTTACTCGTCAAATCGCCACCATGTTG
ATGACTGGCGTCCCTATTGTTCAGGCACTCAAGTTAGTTTCAGAGAACCATAAAAAAGCTGAAATGAAGTCGATTCTAAT
GAGTGTGACGCGAGCGGTAGAAGCAGGGACACCGATGTCGAAAGCCATGCGTACCGCTAGCACGCACTTTGATCCACTTT
ACACCGACTTAATAGCAACAGGTGAGCAGTCCGGTAACTTATCCCAAGTCTTTGAGCGTCTGGCAACCTACCGAGAAAAA
AGCGAGCAGTTGCGTGCAAAGGTTATTAAAGCGCTCATTTACCCGGCGATGGTGGTATTAGTAGCACTTGGGGTTTCTTA
TTTAATGCTGACAAAGGTTATTCCTGAGTTTGAAAAAATGTTTACAGGTTTTGGTGCCGAATTACCCGCCTTCACCCAGA
TGGTACTCAACCTTTCTGCTTGGACCCAAAATTGGGGTCCCTTCATTGGGCTGAGCTTTGTGAGCTTACTCATTTCTGGC
AAAATCATGTCACAACGCTCCGACTCATTTCGTCTTTTGACTGCACGGTCGAGTTTAAGAGTACCTATTTTAGGACCAGT
TTTATCCAAAGCTGCCATTGCGAAGTTTAGCCGTACTTTGGCGACCAGTTTTAGCGCAGGTATTCCTATCCTAACCGCCT
TGAAAACTACCTCAAAAACCTCTGGTAACATGCACTATCAATTGGCAATCGAAGAGGTATATCGAGATACTGCCGCTGGT
ATGCCAATGTACATTGCCATGCGTAACTGTAACGTCTTCCCAGAATTGGTATTGCAAATGGTCATGATTGGTGAAGAGTC
AGGTCGCTTAGATGACATGCTCAACAAGATAGCCGCTATTTATGAGTTCGAAGTCGATAACACCGTCGACAACCTAAGTA
AGATTTTGGAACCGTTGATCATTGTTTTTCTGGGTGTCGTTGTCGGTGGTTTGGTGACAGCAATGTACTTACCAATCTTT
AACTTGATGAGCGTATTGGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K5TX94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

98.28

100

0.983
  pilC Vibrio cholerae strain A1552

74

98.28

0.727
  pilC Acinetobacter baylyi ADP1

42.462

97.789

0.415
  pilC Pseudomonas stutzeri DSM 10701

41.414

97.297

0.403
  pilC Acinetobacter baumannii D1279779

40.447

99.017

0.4
  pilG Neisseria gonorrhoeae MS11

39.558

100

0.396
  pilC Legionella pneumophila strain ERS1305867

39.163

99.754

0.391
  pilG Neisseria meningitidis 44/76-A

38.725

100

0.388