Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PWW23_RS00250 Genome accession   NZ_CP118586
Coordinates   55121..56137 (-) Length   338 a.a.
NCBI ID   WP_029789968.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 50121..61137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW23_RS00225 aroK 50977..51495 (-) 519 WP_005451507.1 shikimate kinase AroK -
  PWW23_RS00230 pilQ 51685..53436 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ family protein Machinery gene
  PWW23_RS00235 pilP 53471..53986 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PWW23_RS00240 pilO 53976..54569 (-) 594 WP_274792385.1 type 4a pilus biogenesis protein PilO Machinery gene
  PWW23_RS00245 pilN 54562..55137 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PWW23_RS00250 pilM 55121..56137 (-) 1017 WP_029789968.1 type IV pilus assembly protein PilM Machinery gene
  PWW23_RS00255 - 56316..58886 (+) 2571 WP_274792384.1 PBP1A family penicillin-binding protein -
  PWW23_RS00260 oxyR 58953..59861 (-) 909 WP_274792383.1 DNA-binding transcriptional regulator OxyR -
  PWW23_RS00265 - 60036..60764 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38023.40 Da        Isoelectric Point: 6.4206

>NTDB_id=793690 PWW23_RS00250 WP_029789968.1 55121..56137(-) (pilM) [Vibrio harveyi strain fish6]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDGYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=793690 PWW23_RS00250 WP_029789968.1 55121..56137(-) (pilM) [Vibrio harveyi strain fish6]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGACAACGCT
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACG
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAACAAGACCCTCTTGCTGAAGACACGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-326)

Type IV pilus assembly protein PilM; (PilM_2)

(8-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.533

100

0.905
  pilM Vibrio cholerae strain A1552

48.83

100

0.494